FlyBase Allele Report: Dmel\bel[GD1324]

General Information

Symbol

Dmel\bel^GD1324

Species

D. melanogaster

Name

FlyBase ID

FBal0209138

Feature type

allele

Associated gene

Dmel\bel

Associated Insertion(s)

Carried in Construct

P{GD1324}

Also Known As

UAS-bel RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD1324}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD1324

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Sertoli cell-only syndrome

CEA

(Kotov et al., 2016)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates fragile X-associated tremor/ataxia syndrome

modeled by Zzzz\CGG^90.UAS.EGFP

(Linsalata et al., 2019)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

atrophy | adult stage, with Scer\GAL4^nanos.PG

(Kotov et al., 2016)

decreased cell number | adult stage, with Scer\GAL4^nanos.PG

(Kotov et al., 2016)

increased cell death | adult stage, with Scer\GAL4^nanos.PG

(Kotov et al., 2016)

increased cell number | adult stage, with Scer\GAL4^NP1624

(Kotov et al., 2016)

lethal, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

lethal, with Scer\GAL4^Act5C.PU

(Dietzl et al., 2007)

lethal, with Scer\GAL4^Bx-MS1096

(Dietzl et al., 2007)

lethal, with Scer\GAL4^GMR.PF, Scer\GAL4^ey.PH

(Dietzl et al., 2007)

lethal, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009, Dietzl et al., 2007)

lethal - all die during larval stage, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

male semi-sterile, with Scer\GAL4^nanos.PG

(Kotov et al., 2016)

some die during embryonic stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

Phenotype Manifest In

male germline cell | adult stage, with Scer\GAL4^nanos.PG

(Kotov et al., 2016)

male germline cell | adult stage, with Scer\GAL4^NP1624

(Kotov et al., 2016)

male germline cell | adult stage | increased number, with Scer\GAL4^NP1624

(Kotov et al., 2016)

male germline stem cell | adult stage, with Scer\GAL4^nanos.PG

(Kotov et al., 2016)

spermatocyte fusome, with Scer\GAL4^nanos.PG

(Kotov et al., 2016)

spermatocyte fusome | adult stage, with Scer\GAL4^NP1624

(Kotov et al., 2016)

testis | adult stage, with Scer\GAL4^nanos.PG

(Kotov et al., 2016)

Detailed Description

Statement

Reference

Expression of bel^GD1324 driven by Scer\GAL4^nos.PG leads to an enlarged hub area, massive loss of germline stem cells (GSCs) and germ cells of successive stages of spermatogenesis in the testes, along with fusome loss or disruption in around 50% of testes; however, maintenance of early somatic and mature cyst cells is similar to controls. Expression of bel^GD1324 driven by Scer\GAL4^nos.PG leads to increased apoptosis in germline cells (but not somatic cyst cells) in the testis compared to controls. Scer\GAL4^nos.PG>bel^GD1324 testes are strongly reduced in size and males are semi-sterile.

Expression of bel^GD1324 driven by Scer\GAL4^tj-NP1624 leads to a complete absence of germ cells in only 5% of testes, with more than half showing an overpopulation of small germ cells (tumor); fusomes and spectrosomes are lost in around 11% of testes.

(Kotov et al., 2016)

Expression under the control of Scer\GAL4^Mef2.PR results in late larval lethality.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in lethality before the pupal stage.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

1324

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^nanos.PG, bel^GD1324 has male semi-sterile phenotype, suppressible | partially by CycB^UAS.cWa, Scer\GAL4^nanos.PG

(Kotov et al., 2016)

Enhancer of

Statement

Reference

bel^GD1324, Scer\GAL4^GMR.PU is an enhancer of visible | adult stage phenotype of Hsap\FUS^UAS.Tag:HA, Scer\GAL4^GMR.PU

(Fortuna et al., 2021)

Phenotype Manifest In

Enhancer of

Statement

Reference

bel^GD1324, Scer\GAL4^GMR.PU is an enhancer of eye phenotype of Hsap\FUS^UAS.Tag:HA, Scer\GAL4^GMR.PU

(Fortuna et al., 2021)

Other

Statement

Reference

CycB^UAS.cWa, Scer\GAL4^nanos.PG, bel^GD1324 has male germline cell | adult stage phenotype

(Kotov et al., 2016)

CycB^UAS.cWa, Scer\GAL4^nanos.PG, bel^GD1324 has testis | adult stage phenotype

(Kotov et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of CycB^Scer\UAS.cWa and bel^GD1324 driven by Scer\GAL4^nos.PG leads to two distinct phenotypes in testes: most (around 75%) are reduced in size and do not contain germ cells, while the remainder have normal testis architecture and germ cell content; male infertility is significantly partially suppressed by co-expression of CycB^Scer\UAS.cWa in Scer\GAL4^nos.PG>bel^GD1324 males.

(Kotov et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v6299

w¹¹¹⁸; P{GD1324}v6299/TM3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

1324

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

bel^GD1324

Name Synonyms

Secondary FlyBase IDs

References (13)

Report Sections

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