UASt regulatory sequences drive expression of an inverted repeat.
Expression of bowlGD1389 under the control of Scer\GAL4klu-G410 results in a reduction in the extent of the prescutum and eliminates lateral microchaetae in the ventral prescutum.
Adults expressing bowlGD1389 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.
Expression of bowlGD1389 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) results in viable flies or partial lethality, depending on the particular P{GD1389} insertion line used.
Expression under the control of Scer\GAL4pnr-MD237 results in extra bristles on the notum in 0% or 10-20% of the Scer\GAL4pnr-MD237 expression domain, depending on the insertion line used.
Expression under the control of Scer\GAL4pnr-MD237 results in planar polarity defects in the orientation of bristles of the notum in 20-30% of the Scer\GAL4pnr-MD237 expression domain.
Expression of bowlGD1389 under the control of Scer\GAL4pnr-MD237 leads to a mild thorax closure phenotype.
Expression of bowlGD1389 under the control of Scer\GAL4ey.PH and Scer\GAL4GMR.PF leads to a mild rough eye phenotype.
Dcr-2UAS.cDa, Scer\GAL4GMR.PF, Scer\GAL4ey.PH, bowlGD1389 has lethal phenotype
Scer\GAL4GMR.PF, Scer\GAL4ey.PH, bowlGD1389 has eye phenotype, enhanceable by Dcr-2UAS.cDa, Scer\GAL4GMR.PF, Scer\GAL4ey.PH
Scer\GAL4pnr-MD237, bowlGD1389 has thorax phenotype, enhanceable by Dcr-2UAS.cDa, Scer\GAL4pnr-MD237
Coexpression of Dcr-2Scer\UAS.cDa with bowlGD1389, under the control of Scer\GAL4ey.PH and Scer\GAL4GMR.PF enhances the bowlGD1389 rough eye phenotype and results in lethality.
Coexpression of Dcr-2Scer\UAS.cDa with bowlGD1389, under the control of Scer\GAL4pnr-MD237, enhances the bowlGD1389 thorax closure phenotype.