UASt regulatory sequences drive expression of an inverted repeat.
The midguts of 10-14 day old flies expressing rkGD15 under the control of Scer\GAL4how-24B (in combination with a Dicer-2 transgene to enhance RNAi efficiency) display intestinal stem cell hyperproliferation and epithelial multilayering, but these flies do not display wing inflation defects, as compared with controls.
Flies expressing rkGD15 under the control of Scer\GAL4Act5C.PI, but not Scer\GAL4Mef2.PU, (in combination with a Dicer-2 transgene to enhance RNAi efficiency) display wing inflation defects.
Adults expressing rkGD15 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.
Scer\GAL4how-24B, rkGD15 has increased cell number phenotype, suppressible by Scer\GAL4how-24B/vnKK108432
Scer\GAL4how-24B, rkGD15 has increased occurrence of cell division | adult stage phenotype, suppressible by Scer\GAL4how-24B/vnKK108432
Scer\GAL4how-24B, rkGD15 has intestinal stem cell phenotype, suppressible by Scer\GAL4how-24B/vnKK108432
Scer\GAL4how-24B, rkGD15 has adult midgut phenotype, suppressible by Scer\GAL4how-24B/vnKK108432
Co-expression of vnKK108432 suppresses the intestinal stem cell hyperproliferation seen in flies expressing rkGD15 under the control of Scer\GAL4how-24B.