UASt regulatory sequences drive expression of an inverted repeat.
Expression under the control of Scer\GAL41407 results in an increased number and size of GFP-positive aggregates within neuroblast lineage cells in the larval brain compared to controls (these aggregates may indicate cell death or a disturbance of internal cellular structures).
Adults expressing snsGD2561 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.
Dcr-2UAS.cDa, Scer\GAL4GMR.PF, Scer\GAL4ey.PH, snsGD2561 has eye phenotype
Scer\GAL4pnr-MD237, snsGD2561 has thorax phenotype
Coexpression of Dcr-2Scer\UAS.cDa with snsGD2561 under the control of Scer\GAL4ey.PH and Scer\GAL4GMR.PF causes an eye phenotype not seen when only snsGD2561 is expressed. Coexpression of the transgenes under the control of Scer\GAL4pnr-MD237 causes a defective thorax closure phenotype.