FB2024_03 , released June 25, 2024
Allele: Dmel\vnGD2321
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General Information
Symbol
Dmel\vnGD2321
Species
D. melanogaster
Name
FlyBase ID
FBal0210867
Feature type
allele
Associated gene
Dmel\vn
Associated Insertion(s)
Carried in Construct
P{GD2321}
Key Links
Genomic Maps

Transgenic product class
RNAi_reagent
(Dickson et al., 2007.7.18)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Dickson et al., 2007.7.18)
Mutagen
in vitro construct
(Dickson et al., 2007.7.18)
Progenitor genotype
Carried in construct
P{GD2321}
Cytology
Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
dsRNA-GD2321
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of vnGD2321 RNAi under the control of Scer\GAL4Act5C.PU (using tub-Gal80[ts] to limit the RNAi expression to the adult stage) results in germline stem cell differentiation defects in adult testes.

      (Liu et al., 2016)

      Expression of vnGD2321 under the control of Scer\GAL4repo.PL or Scer\GAL4nrv2.PS result in significantly decreased axon wrapping index (proportion of axon clusters wrapped by wrapping glia), whereas expression under Scer\GAL4elav.PU does not affect the axon wrapping by the wrapping glia in third instar larvae.

      In wild-type larvae, subperineurial glial cells (SPGs) form autocellular contacts with pronounced septate junction, the length of these autocellular contacts is significantly reduced upon Scer\GAL4repo.PL or Scer\GAL4nrv2.PS-driven knock-down of vn, while expression of vnGD2321 controlled by Scer\GAL4moody.SPG (in SPGs) has no effect.

      (Matzat et al., 2015)

      Flies expressing vnGD2321 under the control of Scer\GAL4sd.PU exhibit wing vein phenotypes. There is loss of L4, L2 and the anterior crossvein. Less severe vein loss is seen when vnGD2321 is expressed under the control of Scer\GAL469B.

      (Austin et al., 2014)

      Expression of vnGD2321 in visceral muscle cells under the control of Scer\GAL80ts.αTub84B and Scer\GAL4how-24B does not affect midgut development and renewal, even after Pseudomonas entomophila infection.

      (Jiang et al., 2011)

      Adults expressing vnGD2321 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

      (Neely et al., 2010)

      Expression of vnGD2321 in visceral muscle cells under the control of Scer\GAL4how-24B and Scer\GAL80ts.αTub84B throughout larval development results in late larval midguts with very few adult midgut progenitor cell clusters. Induction of vnGD2321 under the control of Scer\GAL4how-24B and Scer\GAL80ts.αTub84B starting at early third instar has no effect on adult midgut progenitor cells.

      Expression of vnGD2321 under the control of Scer\GAL4esg-NP7397 and Scer\GAL80ts.αTub84B has no effect on adult midgut progenitor cell proliferation.

      Expression of vnGD2321 under the control of Scer\GAL4Myo31DF-NP0001 and Scer\GAL80ts.αTub84B has no effect on adult midgut progenitor cell proliferation.

      (Jiang and Edgar, 2009)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT Enhanced by
      Statement
      Reference

      Scer\GAL4sd.PU, vnGD2321 has visible phenotype, non-enhanceable by spiHMS01120/Scer\GAL4sd.PU

      (Austin et al., 2014)

      Scer\GAL4sd.PU, vnGD2321 has visible phenotype, non-enhanceable by Scer\GAL4sd.PU/spiJF03322

      (Austin et al., 2014)
      Phenotype Manifest In
      NOT Enhanced by
      Statement
      Reference

      Scer\GAL4sd.PU, vnGD2321 has wing vein phenotype, non-enhanceable by spiHMS01120/Scer\GAL4sd.PU

      (Austin et al., 2014)

      Scer\GAL4sd.PU, vnGD2321 has wing vein phenotype, non-enhanceable by Scer\GAL4sd.PU/spiJF03322

      (Austin et al., 2014)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of spiHMS01120 does not enhance the wing vein phenotypes seen when vnGD2321 is expressed under the control of Scer\GAL4sd.PU.

      Expression of spiJF03322 does not enhance the wing vein phenotypes seen when vnGD2321 is expressed under the control of Scer\GAL4sd.PU.

      (Austin et al., 2014)

      Knockdown of vn through expression of vnGD2321 in Df(3L)Krn-27-7-B mutant visceral muscle cells (under the control of Scer\GAL4how-24B and Scer\GAL80ts.αTub84B) significantly reduces intestinal stem cell proliferation.

      (Jiang et al., 2011)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      veinRNAi
      (Matzat et al., 2015)
      vnGD2321
      Name Synonyms
      Secondary FlyBase IDs
        References (11)