FlyBase Allele Report: Dmel\wb[GD1560]

General Information

Symbol

Dmel\wb^GD1560

Species

D. melanogaster

Name

FlyBase ID

FBal0210877

Feature type

allele

Associated gene

Dmel\wb

Associated Insertion(s)

Carried in Construct

P{GD1560}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD1560}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD1560

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^hh-Gal4

(Han et al., 2012)

abnormal size | adult stage, with Scer\GAL4^nub.PU

(Hevia et al., 2017)

lethal, with Scer\GAL4^Act5C.PU

(Dietzl et al., 2007)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

chaeta | increased number, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

larval dorsal multidendritic neuron ddaC | third instar larval stage, with Scer\GAL4^hh-Gal4

(Han et al., 2012)

wing, with Scer\GAL4^Bx-MS1096

(Dietzl et al., 2007)

wing, with Scer\GAL4^nub.PU

(Hevia et al., 2017)

Detailed Description

Statement

Reference

The expression of wb^GD1560 under the control of Scer\GAL4^nub.PU (together with Dicer-2, for efficient RNAi) leads to small, folded and blistered wings, as compared to controls.

(Hevia et al., 2017)

When wb^GD1560 is expressed in the posterior epidermal cells of third instar larvae using Scer\GAL4^hh-Gal4 21.36% of Class IV ddaC dendrites are enclosed by the epidermis, rather than attached to the ECM.

(Han et al., 2012)

Adults expressing wb^GD1560 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in extra bristles on the notum in 10-20% of the Scer\GAL4^pnr-MD237 expression domain.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 20-30% of the Scer\GAL4^pnr-MD237-expressing area of the notum.

(Mummery-Widmer et al., 2009)

Expression of wb^GD1560 under the control of Scer\GAL4^Bx-MS1096 leads to crinkled and dusky wings.

(Dietzl et al., 2007)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

1560

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

wb^GD1560/Scer\GAL4^nub.PU is an enhancer of abnormal size | adult stage phenotype of Scer\GAL4^nub.PU, Smox^SDVD.UAS

(Hevia et al., 2017)

Other

Statement

Reference

Dcr-2^UAS.cDa, Scer\GAL4^pnr-MD237, wb^GD1560 has lethal phenotype

(Dietzl et al., 2007)

Phenotype Manifest In

Enhancer of

Statement

Reference

wb^GD1560/Scer\GAL4^nub.PU is an enhancer of wing phenotype of Scer\GAL4^nub.PU, Smox^SDVD.UAS

(Hevia et al., 2017)

Other

Statement

Reference

Dcr-2^UAS.cDa, Scer\GAL4^GMR.PF, Scer\GAL4^ey.PH, wb^GD1560 has eye phenotype

(Dietzl et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

The co-expression of wb^GD1560 (together with Dicer-2, for efficient RNAi) enhances the oversized wing defects induced by the expression of Smox^{SDVD.Scer\UAS} under the control of Scer\GAL4^nub.PU.

(Hevia et al., 2017)

Coexpression of Dcr-2^Scer\UAS.cDa with wb^GD1560, under the control of Scer\GAL4^ey.PH and Scer\GAL4^GMR.PF leads to a rough eye and necrotic eye phenotype, while expression of only wb^GD1560 does not cause an eye phenotype.

Coexpression of Dcr-2^Scer\UAS.cDa and wb^GD1560, under the control of Scer\GAL4^pnr-MD237, leads to lethality while Scer\GAL4^pnr-MD237>wb^GD1560 flies are viable.

(Dietzl et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v3141

w¹¹¹⁸; P{GD1560}v3141

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

1560

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

wb^GD1560

Name Synonyms

Secondary FlyBase IDs

References (11)

Report Sections

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