FB2024_02 , released April 23, 2024
Allele: Dmel\DnaJ-1UAS.Tag:FLAG
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General Information
Symbol
Dmel\DnaJ-1UAS.Tag:FLAG
Species
D. melanogaster
Name
FlyBase ID
FBal0212787
Feature type
allele
Associated gene
Dmel\DnaJ-1
Associated Insertion(s)
Carried in Construct
P{UAS-DnaJ-1.FLAG}
Key Links
Transgenic product class
cDNA
(Chan et al., 2000)
wild_type
(Chan et al., 2000)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Chan et al., 2000)
wild_type
(Chan et al., 2000)
Mutagen
in vitro construct - regulatory fusion
(Bonini, 2002, Chan et al., 2000)
in vitro construct - coding region fusion
(Chan et al., 2000)
Progenitor genotype
Carried in construct
P{UAS-DnaJ-1.FLAG}
Cytology
Description

UASt regulatory sequences drive expression of a DnaJ-1 cDNA tagged at the C-terminal end with Tag:FLAG.

(Chan et al., 2000)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
DnaJ-1
Tagged with
Tag:FLAG
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Flies expressing DnaJ-1Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4GMR.PU have normal external eye morphology.

      (Chan et al., 2000)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressor of
      NOT Suppressor of
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      Co-expression of DnaJ-1Scer\UAS.T:Zzzz\FLAG partially suppresses the eye degeneration seen in flies expressing Hsap\MJDtr.Q78.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PF.

      Co-expression of both Hsap\HSPA1LScer\UAS.cWa and DnaJ-1Scer\UAS.T:Zzzz\FLAG completely suppresses the eye degeneration seen in flies expressing Hsap\MJDtr.Q78.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PF.

      (Bonini, 2002)

      Co-expression of DnaJ-1Scer\UAS.T:Zzzz\FLAG restores external eye structure in animals expressing Hsap\ATXN3tr.Q78.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PU. Internal eye structure is partially rescued, with partial preservation of photoreceptor rhabdomere specialisations.

      Co-expression of DnaJ-1Scer\UAS.T:Zzzz\FLAG slow the progressive retinal degeneration seen in flies that weakly express Hsap\ATXN3tr.Q78.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PU.

      Expression of DnaJ-1Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4elav-C155 only slightly suppresses the retinal degeneration seen in 10 day old flies expressing Hsap\HTTGMR.Q120.

      Expression of DnaJ-1Scer\UAS.T:Zzzz\FLAG cannot suppress the severe eye degeneration seen in flies co-expressing Hsap\ATXN3tr.Q78.Scer\UAS.T:Ivir\HA1 and Hsc70-4K71S.Scer\UAS under the control of Scer\GAL4GMR.PU.

      Simultaneous co-expression of both DnaJ-1Scer\UAS.T:Zzzz\FLAG and Hsap\HSPA1LScer\UAS.cWa results in a stronger suppression of the retinal degeneration caused by expression of Hsap\ATXN3tr.Q78.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PU than expression of either construct alone.

      (Chan et al., 2000)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      DnaJ-1Scer\UAS.T:Zzzz\FLAG
      DnaJ-1UAS.Tag:FLAG
      Name Synonyms
      Secondary FlyBase IDs
        References (2)