FlyBase Allele Report: Dmel\Ced-12[KO]

General Information

Symbol

Dmel\Ced-12^KO

Species

D. melanogaster

Name

FlyBase ID

FBal0213142

Feature type

allele

Associated gene

Dmel\Ced-12

Associated Insertion(s)

TI{TI}Ced-12^KO

Carried in Construct

Also Known As

elmo^KO

Key Links

Allele class

loss of function allele

Mutagen

Nature of the Allele

Allele class

loss of function allele

(Bianco et al., 2007)

Mutagen

recombination

(Bianco et al., 2007)

gene targeting by homologous recombination

(Bianco et al., 2007)

Progenitor genotype

Associated Insertion(s)

TI{TI}Ced-12^KO

Cytology

Description

Most of the Ced-12 gene has been replaced by a w[+] marker.

(Bianco et al., 2007)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die during embryonic stage | germline clone

(Biersmith et al., 2011)

some die during embryonic stage | germline clone

(Biersmith et al., 2011)

Phenotype Manifest In

border follicle cell | somatic clone

(Bianco et al., 2007)

visceral muscle fiber | embryonic stage (with Ced-12^19F3)

(Liu et al., 2013)

Detailed Description

Statement

Reference

Detached muscle fibres are present in Ced-12^19F3/Ced-12^KO zygotic mutant stage 16-17 embryos.

Stage 16-17 Ced-12^19F3/Ced-12^KO zygotic mutant embryos exhibit muscle attachment sites that are reduced in size and a decreased number of mature tendon cells.

(Liu et al., 2013)

Longitudinal fascicles in embryos homozygous for Ced-12^KOappear normal.

Embryos derived from Ced-12^KO germline clones show early lethality.

(Biersmith et al., 2011)

The level of phagocytosis occurring in the hemocytes of mutant embryos is normal.

(Kuraishi et al., 2009)

Clusters of mutant border cells (induced as clones) arrest early in migration in the egg chamber.

Ced-12^KO mutant cells are generally located at the rear of the cluster during border cell posterior migration in mosaic border cell clusters consisting of both wild-type and mutant cells. During dorsal migration of these mosaic border cell clusters the mutant cells are equally frequently in the leading and rear positions.

(Bianco et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

Ced-12^KO/Ced-12^19F3 has visceral muscle fiber | embryonic stage phenotype, enhanceable by msk⁴/msk[+]

(Liu et al., 2013)

Enhancer of

Statement

Reference

Ced-12^KO/Ced-12[+] is an enhancer of visceral muscle fiber | embryonic stage phenotype of msk⁴/msk⁵

(Liu et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

The presence of msk⁴/+ increases the severity of muscle attachment defects in Ced-12^19F3/Ced-12^KO embryos.

The presence of Ced-12^KO/+ increases the severity of muscle attachment defects in msk⁴/msk⁵ embryos.

(Liu et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Ced-12^KO is rescued by Ced-12^UAS.cBa/Scer\GAL4^Tub.PU

(Bianco et al., 2007)

Partially rescued by

Ced-12^KO/Ced-12^19F3 is partially rescued by Scer\GAL4^Mef2.PR/Ced-12^UAS.cGa

(Liu et al., 2013)

Ced-12^KO/Ced-12^19F3 is partially rescued by Scer\GAL4^how-24B/Ced-12^UAS.cGa

(Liu et al., 2013)

Ced-12^KO/Ced-12^19F3 is partially rescued by Scer\GAL4^Mef2.PR/Ced-12^ΔN.UAS

(Liu et al., 2013)

Ced-12^KO/Ced-12^19F3 is partially rescued by Scer\GAL4^how-24B/Ced-12^ΔN.UAS

(Liu et al., 2013)

Not rescued by

Ced-12^KO/Ced-12^19F3 is not rescued by Scer\GAL4^sr-md710/Ced-12^UAS.cGa

(Liu et al., 2013)

Ced-12^KO/Ced-12^19F3 is not rescued by Ced-12^ΔC.UAS/Scer\GAL4^Mef2.PR

(Liu et al., 2013)

Ced-12^KO/Ced-12^19F3 is not rescued by Scer\GAL4^how-24B/Ced-12^ΔC.UAS

(Liu et al., 2013)

Comments

Scer\GAL4^sr-md710-mediated expression of Ced-12^Scer\UAS.cGa in tendon cells does not improve the muscle detachment phenotype of Ced-12^19F3/Ced-12^KO embryos.

Scer\GAL4^Mef2.PR- or Scer\GAL4^how-24B-mediated expression of Ced-12^Scer\UAS.cGa in muscles or mesoderm, respectively, efficiently but not completely rescues the muscle detachment phenotype of Ced-12^19F3/Ced-12^KO embryos.

Scer\GAL4^Mef2.PR- or Scer\GAL4^how-24B-mediated expression of Ced-12^ΔC.Scer\UAS in muscles or mesoderm, respectively, does not improve the muscle detachment phenotype of Ced-12^19F3/Ced-12^KO embryos.

(Liu et al., 2013)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Ced-12^KO

(Timmons et al., 2016)

elmo^KO

(Liu et al., 2013, Biersmith et al., 2011, Kuraishi et al., 2009, Bianco et al., 2007)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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