FB2024_03 , released June 25, 2024
Allele: Dmel\Ced-12UAS.cGa
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General Information
Symbol
Dmel\Ced-12UAS.cGa
Species
D. melanogaster
Name
FlyBase ID
FBal0266886
Feature type
allele
Associated gene
Dmel\Ced-12
Associated Insertion(s)
Carried in Construct
P{UAS-Ced-12.G}
Also Known As
UAS-elmo
Key Links
Transgenic product class
cDNA
(Geisbrecht et al., 2008)
wild_type
(Geisbrecht et al., 2008)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Geisbrecht et al., 2008)
wild_type
(Geisbrecht et al., 2008)
Mutagen
in vitro construct
(Geisbrecht et al., 2008)
Progenitor genotype
Carried in construct
P{UAS-Ced-12.G}
Cytology
Description

UASt drives expression of a full-length Ced-12 cDNA.

(Geisbrecht et al., 2008)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Ced-12
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Scer\GAL4Mef2.PR-mediated expression of Ced-12Scer\UAS.cGa does not cause any obvious muscle perturbations.

      Scer\GAL4GMR.PU-mediated expression of Ced-12Scer\UAS.cGa does not impact on ommatidial organization.

      (Geisbrecht et al., 2008)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressed by
      Statement
      Reference

      Ced-12UAS.cGa, Scer\GAL4Mef2.PR, mbcUAS.cBa has visceral muscle fiber | embryonic stage phenotype, suppressible by Rac1J11/Rac2Δ/Rac2[+]/Rac1[+]

      (Liu et al., 2013)

      Ced-12UAS.cGa, Scer\GAL4Mef2.PR, mbcUAS.cBa has tendon cell | embryonic stage phenotype, suppressible by Rac1J11/Rac2Δ/Rac2[+]/Rac1[+]

      (Liu et al., 2013)

      Ced-12UAS.cGa, Scer\GAL4Mef2.PR, mbcUAS.cBa has eye phenotype, suppressible by Ced-12[+]/Ced-1219F3

      (Geisbrecht et al., 2008)

      Ced-12UAS.cGa, Scer\GAL4Mef2.PR, mbcUAS.cBa has eye phenotype, suppressible by mbcD11.2/mbc[+]

      (Geisbrecht et al., 2008)

      Ced-12UAS.cGa, Scer\GAL4Mef2.PR, mbcUAS.cBa has eye phenotype, suppressible by Rac1J11/Rac2Δ/Rac2[+]/Rac1[+]

      (Geisbrecht et al., 2008)
      NOT suppressed by
      Statement
      Reference

      Ced-12UAS.cGa, Scer\GAL4Mef2.PR, mbcUAS.cBa has visceral muscle fiber | embryonic stage phenotype, non-suppressible by msk4/msk[+]

      (Liu et al., 2013)

      Ced-12UAS.cGa, Scer\GAL4Mef2.PR, mbcUAS.cBa has tendon cell | embryonic stage phenotype, non-suppressible by msk4/msk[+]

      (Liu et al., 2013)
      Suppressor of
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of Ced-12Scer\UAS.cGa and mbcScer\UAS.cBa via Scer\GAL4Mef2.PR results in prevalent myoblast fusion, muscle attachment, and tendon cell identity phenotypes in embryos. The muscle attachment and tendon cell phenotypes are largely rescued in a Rac1J11/+, Rac2Δ/+ background, but not in a msk4/+ background.

      Expression of Ced-12Scer\UAS.cGa or Ced-12Scer\UAS.cGa together with mbcScer\UAS.cBa via Scer\GAL4Mef2.PR partially rescues the msk4 embryonic muscle detachment phenotype.

      (Liu et al., 2013)

      Co-expression of Ced-12Scer\UAS.cGa and mbcScer\UAS.cBa using Scer\GAL4Mef2.PR results in missing muscles and many unfused myoblasts.

      Co-expression of Ced-12Scer\UAS.cGa and mbcScer\UAS.cBa using Scer\GAL4GMR.PU causes a rough eye phenotype.

      The rough eye phenotype resulting from Scer\GAL4GMR.PU-mediated expression of Ced-12Scer\UAS.cGa and mbcScer\UAS.cBa is suppressed by heterozygosity for Ced-1219F3, mbcD11.2 or Rac1J11, Rac2Δ.

      (Geisbrecht et al., 2008)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Scer\GAL4Act.PU/Ced-12UAS.cGa rescues Ced-12c06760

      (Geisbrecht et al., 2008)
      Partially rescues

      Scer\GAL4Mef2.PR/Ced-12UAS.cGa partially rescues Ced-12KO/Ced-1219F3

      (Liu et al., 2013)

      Scer\GAL4how-24B/Ced-12UAS.cGa partially rescues Ced-12KO/Ced-1219F3

      (Liu et al., 2013)
      Fails to rescue

      Scer\GAL4sr-md710/Ced-12UAS.cGa fails to rescue Ced-12KO/Ced-1219F3

      (Liu et al., 2013)
      Comments

      Scer\GAL4sr-md710-mediated expression of Ced-12Scer\UAS.cGa in tendon cells does not improve the muscle detachment phenotype of Ced-1219F3/Ced-12KO embryos.

      Scer\GAL4Mef2.PR- or Scer\GAL4how-24B-mediated expression of Ced-12Scer\UAS.cGa in muscles or mesoderm, respectively, efficiently but not completely rescues the muscle detachment phenotype of Ced-1219F3/Ced-12KO embryos.

      (Liu et al., 2013)

      Expression of Ced-12Scer\UAS.cGa under the control of Scer\GAL4Act.PU reverts the lethality of Ced-12c06760.

      (Geisbrecht et al., 2008)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Ced-12Scer\UAS.cGa
      Ced-12UAS.cGa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Geisbrecht
      (Geisbrecht et al., 2008)
      Secondary FlyBase IDs
        References (3)