FlyBase Allele Report: Dmel\mbc[D11.2]

General Information

Symbol

Dmel\mbc^D11.2

Species

D. melanogaster

Name

FlyBase ID

FBal0063880

Feature type

allele

Associated gene

Dmel\mbc

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Bour et al., 2000)

Mutagen

ethyl methanesulfonate

(Balagopalan et al., 2006, Erickson et al., 1997)

Progenitor genotype

Cytology

Description

Nucleotide substitution: G?A.

(Balagopalan et al., 2006)

Amino acid replacement: W97term.

(Balagopalan et al., 2006)

Amino acid replacement: E97term.

(Erickson et al., 1997)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:23,792,195..23,792,195 [+]

Nucleotide change:

G23792195A

Reported nucleotide change:

G?A

Amino acid change:

W97term | mbc-PA; W97term | mbc-PB

Reported amino acid change:

W97term

Comment:

TGG to TGA nonsense mutation in codon Trp97.

(Erickson et al., 1997)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | embryonic stage

(Biersmith et al., 2011)

Phenotype Manifest In

border follicle cell | somatic clone

(Duchek et al., 2001)

dorsal pharyngeal muscle cell

(Haralalka et al., 2014)

embryonic/larval midgut longitudinal visceral muscle cell | embryonic stage (with mbc^C1)

(Rudolf et al., 2014)

embryonic midgut constriction 1 | embryonic stage (with mbc^C1)

(Rudolf et al., 2014)

embryonic myoblast

(Biersmith et al., 2011, Haralalka et al., 2011, Balagopalan et al., 2006)

embryonic myoblast | germline clone

(Balagopalan et al., 2006)

lateral longitudinal fascicle

(Biersmith et al., 2011)

longitudinal visceral muscle primordium (with mbc^C1)

(Rudolf et al., 2014)

muscle cell of A1-7 dorsal acute muscle 1 | embryonic stage 15

(Menon et al., 2005)

muscle founder cell | embryonic stage

(Shelton et al., 2009)

presumptive embryonic/larval muscle system

(Balagopalan et al., 2006, Menon et al., 2005)

presumptive embryonic/larval muscle system | germline clone

(Balagopalan et al., 2006)

Detailed Description

Statement

Reference

Mutant stage 16 embryos show a severe myoblast fusion defect in the dorsal pharyngeal muscle.

(Haralalka et al., 2014)

mbc^C1/mbc^D11.2 transheterozygote embryos display defects in the migration of the visceral longitudinal muscle founder cells and their morphology as well as the muscle fusion. Late in the embryonic development, the first midgut constriction is missing in the mbc^C1/mbc^D11.2 mutants.

(Rudolf et al., 2014)

In homozygous mutant embryos, myoblasts are competent to migrate to the founder cells, but fusion does not occur.

Homozygous embryos exhibit breaks in the outer longitudinal fascicles and a collapse of axons onto the MP1 fascicle tracts. Thinning of the longitudinal axon tracts and abnormal spacing between segments is also observed.

(Biersmith et al., 2011)

Unfused myoblasts are apparent in stage 16 homozygous embryos. Although the majority of founder cells examined remain mononucleate, a limited amount of fusion does occur, as binucleate muscle precursors that have undergone a single fusion event between a founder cell and a fusion competent myoblast (FCM) are seen in the mutant embryos. The muscle founders analysed and the percentage of segments in which the founder cell remains mononucleate are as follows: DA1 (72.1%), DO1 (76.3%), LO1 (90.2%), LT2 (93.4%), LT4 (95.0%), VT1 (78.9%), VA2 (84.6%). The overall level of myoblast fusion in embryos lacking both maternal and zygotic function is roughly comparable to that seen in zygotic mutant embryos.

The F-actin foci which are seen in wild-type FCMs at points of contact with developing myofibers are less dense and somewhat dispersed in homozygous FCMs. In addition, the actin cytoskeletal network of the mutant FCMs is different from wild type, with many of the mutant cells showing a complete collapse of the network.

(Haralalka et al., 2011)

The eve-expressing DA1 founder cell undergoes no fusion in mbc^D11.2 embryos.

(Shelton et al., 2009)

Myoblast fusion is absent in mbc^D11.2 zygotic mutant embryos and in embryos derived from mbc^D11.2 germline clones.

(Balagopalan et al., 2006)

The extent of myoblast fusion in mbc^D11.2 mutant embryos is significantly reduced compared to wild type.

(Menon et al., 2005)

Homozygous embryos do not show any apparent defects in macrophage migration.

(Paladi and Tepass, 2004)

When mutant somatic clones are made in border cells, a strong effect is seen on border cell migration. At stage 10 only about 10% of mutant border cell clusters reach the oocyte.

(Duchek et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

mbc^D11.2 has abnormal neuroanatomy | embryonic stage phenotype, enhanceable by spg²

(Biersmith et al., 2011)

NOT Enhanced by

Statement

Reference

mbc^D11.2 has abnormal neuroanatomy | embryonic stage phenotype, non-enhanceable by Df(2L)CadN^Δ14

(Biersmith et al., 2011)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

mbc^D11.2 has embryonic myoblast phenotype, non-enhanceable by spg²

(Biersmith et al., 2011)

mbc^D11.2 has lateral longitudinal fascicle phenotype, non-enhanceable by spg²

(Biersmith et al., 2011)

mbc^D11.2 has lateral longitudinal fascicle phenotype, non-enhanceable by Df(2L)CadN^Δ14

(Biersmith et al., 2011)

Suppressed by

Statement

Reference

mbc^D11.2 has embryonic myoblast phenotype, suppressible by Rac1^V12.UAS/Scer\GAL4^sns.PK

(Haralalka et al., 2011)

NOT suppressed by

Statement

Reference

mbc^D11.2 has embryonic myoblast phenotype, non-suppressible by Scer\GAL4^{kirre-rP298-G4}/Rac1^V12.UAS

(Haralalka et al., 2011)

mbc^D11.2 has embryonic myoblast phenotype, non-suppressible by Scer\GAL4^sns.PK/Rac1^UAS.cLa

(Haralalka et al., 2011)

Enhancer of

Statement

Reference

mbc^D11.2/mbc[+] is an enhancer of visceral muscle fiber | embryonic stage phenotype of msk⁴/msk⁵

(Liu et al., 2013)

mbc^D11.2 is an enhancer of mesothoracic tergum phenotype of Pvr^RNAi.UAS, Scer\GAL4^pnr-MD237

(Ishimaru et al., 2004)

mbc^D11.2 is an enhancer of thorax phenotype of Pvr^RNAi.UAS, Scer\GAL4^pnr-MD237

(Ishimaru et al., 2004)

Suppressor of

Statement

Reference

mbc^D11.2/mbc[+] is a suppressor of visceral muscle fiber | embryonic stage phenotype of Scer\GAL4^twi.PU, msk^UAS.cLa

(Liu et al., 2013)

mbc^D11.2/mbc[+] is a suppressor of eye phenotype of Ced-12^UAS.cGa, Scer\GAL4^Mef2.PR, mbc^UAS.cBa

(Geisbrecht et al., 2008)

mbc^D11.2 is a suppressor of centripetally migrating follicle cell & actin filament phenotype of Pvr^{λ.UASp.Tag:MYC}, Scer\GAL4^slbo.2.6

(Duchek et al., 2001)

mbc^D11.2 is a suppressor of centripetal follicle cell phenotype of Pvr^{λ.UASp.Tag:MYC}, Scer\GAL4^slbo.2.6

(Duchek et al., 2001)

Other

Statement

Reference

mbc^D11.2, spg² has larval ventral nerve cord | embryonic stage phenotype

(Biersmith et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

The presence of mbc^D11.2/+ increases the severity of muscle attachment defects in msk⁴/msk⁵ embryos.

mbc^D11.2/+ largely suppresses the muscle detachment phenotype of Scer\GAL4^twi.PU msk^Scer\UAS.cLa embryos.

(Liu et al., 2013)

In mbc^D11.2/mbc^D11.2, spg²/spg² double mutants, myoblasts fail to fuse but still cluster around the founder cells (as in mbc^D11.2/mbc^D11.2 mutants).

There is no significant increase in broken fascicles or the collapse of the outer longitudinal tracts in mbc^D11.2, spg² double mutants over mbc^D11.2 mutants alone. However, there is an increase in midline fascicle crossing in the double mutants. Abnormal positioning of the ventral nerve cord is also observed in the double mutants.

mbc^D11.2, Df(2L)CadN^Δ14 embryos do not show increased axonal outgrowth or guidance defects compared to mbc^D11.2 embryos.

(Biersmith et al., 2011)

Expression of Rac1^V12.Scer\UAS under the control of Scer\GAL4^sns.PK rescues myoblast fusion in homozygous mbc^D11.2 embryos such that the somatic muscle pattern is almost wild type.

Expression of Rac1^V12.Scer\UAS under the control of Scer\GAL4^kirre-rP298 does not rescue myoblast fusion in homozygous mbc^D11.2 embryos.

Expression of Rac1^Scer\UAS.cLa under the control of Scer\GAL4^sns.PK does not rescue myoblast fusion in homozygous mbc^D11.2 embryos.

mbc^D11.2 spg² double mutant embryos have a roughly comparable overall level of myoblast fusion compared to mbc^D11.2 single mutant embryos.

(Haralalka et al., 2011)

The rough eye phenotype resulting from Scer\GAL4^GMR.PU-mediated expression of Ced-12^Scer\UAS.cGa and mbc^Scer\UAS.cBa is suppressed by heterozygosity for mbc^D11.2.

(Geisbrecht et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

mbc^D11.2 is rescued by mbc^UAS.Tag:HA/Scer\GAL4^sns.PK

(Haralalka et al., 2011)

mbc^D11.2 is rescued by mbc^{NPxxP.Δ1807.UAS.Tag:HA}/Scer\GAL4^sns.PK

(Haralalka et al., 2011)

mbc^D11.2 is rescued by mbc^UAS.Tag:HA/Scer\GAL4^twi.PG

(Balagopalan et al., 2006)

mbc^D11.2 is rescued by Scer\GAL4^twi.PG/mbc^CBS.UAS

(Balagopalan et al., 2006)

mbc^D11.2 is rescued by Scer\GAL4^twi.PG/mbc^{Δ1807.UAS.Tag:HA}

(Balagopalan et al., 2006)

mbc^D11.2 is rescued by mbc^{NPxxP.UAS.Tag:HA}/Scer\GAL4^twi.PG

(Balagopalan et al., 2006)

mbc^D11.2 is rescued by Scer\GAL4^twi.PG/mbc^{NPxxP.Δ1807.UAS.Tag:HA}

(Balagopalan et al., 2006)

mbc^D11.2 is rescued by Scer\GAL4^twi.PG/mbc^{ΔPRM.UAS.Tag:HA}

(Balagopalan et al., 2006)

mbc^D11.2/Df(3R)mbc⁹ is rescued by mbc^UAS.Tag:HA/Scer\GAL4^how-24B

(Balagopalan et al., 2006)

mbc^D11.2/Df(3R)mbc⁹ is rescued by Scer\GAL4^how-24B/mbc^CBS.UAS

(Balagopalan et al., 2006)

mbc^D11.2/Df(3R)mbc⁹ is rescued by Scer\GAL4^how-24B/mbc^{Δ1807.UAS.Tag:HA}

(Balagopalan et al., 2006)

mbc^D11.2/Df(3R)mbc⁹ is rescued by mbc^{NPxxP.UAS.Tag:HA}/Scer\GAL4^how-24B

(Balagopalan et al., 2006)

mbc^D11.2/Df(3R)mbc⁹ is rescued by Scer\GAL4^how-24B/mbc^{NPxxP.Δ1807.UAS.Tag:HA}

(Balagopalan et al., 2006)

mbc^D11.2/Df(3R)mbc⁹ is rescued by Scer\GAL4^how-24B/mbc^{ΔPRM.UAS.Tag:HA}

(Balagopalan et al., 2006)

Not rescued by

mbc^D11.2 is not rescued by mbc^UAS.Tag:HA/Scer\GAL4^{kirre-rP298-G4}

(Haralalka et al., 2011)

mbc^D11.2 is not rescued by mbc^UAS.Tag:HA/Scer\GAL4^{kirre-rP298-G4}

(Balagopalan et al., 2006)

mbc^D11.2 is not rescued by Scer\GAL4^twi.PG/mbc^{SH3W47K.UAS.Tag:HA}

(Balagopalan et al., 2006)

mbc^D11.2 is not rescued by Scer\GAL4^twi.PG/mbc^{DockerF6.4.UAS.Tag:HA}

(Balagopalan et al., 2006)

mbc^D11.2 is not rescued by Scer\GAL4^twi.PG/mbc^{ΔDHR1.UAS.Tag:HA}

(Balagopalan et al., 2006)

mbc^D11.2/Df(3R)mbc⁹ is not rescued by Scer\GAL4^how-24B/mbc^{ΔDHR1.UAS.Tag:HA}

(Balagopalan et al., 2006)

Comments

Expression of either mbc^{Scer\UAS.T:Ivir\HA1} or mbc^{NPxxP.Δ1807.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^sns.PK restores fusion in mbc^D11.2 embryos and a wild-type somatic muscle pattern is formed.

Myoblast fusion remains severely impaired in mbc^D11.2 embryos expressing mbc^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^kirre-rP298. These embryos show a roughly twofold increase in muscle precursor formation (where a single fusion event between a founder cell and a fusion competent myoblast has occurred) compared to mbc^D11.2 embryos.

(Haralalka et al., 2011)

Expression of mbc^{Scer\UAS.T:Ivir\HA1} rescues the myoblast fusion defect in mbc^D11.2 embryos when expressed using Scer\GAL4^twi.PG or Scer\GAL4^how-24B but not Scer\GAL4^kirre-rP298.

Scer\GAL4^twi.PG-mediated expression of mbc^{SH3W47K.Scer\UAS.T:Ivir\HA1} fails to rescue the mbc^D11.2 myoblast fusion defect.

Scer\GAL4^twi.PG-mediated expression of mbc^{DockerF6.4.Scer\UAS.T:Ivir\HA1} fails to rescue the mbc^D11.2 myoblast fusion defect.

Scer\GAL4^twi.PG- or Scer\GAL4^how-24B-mediated expression of mbc^CBS.Scer\UAS rescues the mbc^D11.2 myoblast fusion defect.

Scer\GAL4^twi.PG- or Scer\GAL4^how-24B-mediated expression of mbc^{Δ1807.Scer\UAS.T:Ivir\HA1} rescues the mbc^D11.2 myoblast fusion defect.

Scer\GAL4^twi.PG- or Scer\GAL4^how-24B-mediated expression of mbc^{NPxxP.Scer\UAS.T:Ivir\HA1} rescues the mbc^D11.2 myoblast fusion defect.

Scer\GAL4^twi.PG- or Scer\GAL4^how-24B-mediated expression of mbc^{NPxxP.Δ1807.Scer\UAS.T:Ivir\HA1} rescues the mbc^D11.2 myoblast fusion defect.

Scer\GAL4^twi.PG- or Scer\GAL4^how-24B-mediated expression of mbc^{ΔPRM.Scer\UAS.T:Ivir\HA1} rescues the mbc^D11.2 myoblast fusion defect.

Scer\GAL4^twi.PG- or Scer\GAL4^how-24B-mediated expression of mbc^{ΔDHR1.Scer\UAS.T:Ivir\HA1} fails to rescue the mbc^D11.2 myoblast fusion defect.

(Balagopalan et al., 2006)

Images (0)

Mutant

Wild-type

Stocks (4)

Bloomington

4952

st¹ e¹ mbc^D11.2/TM3, Ser¹

92184

y¹ w^*; P{UAS-mbc.HA}267, mbc^D11.2/TM3, P{HZR+6.8Xb}JG1, Sb¹

92199

w¹¹¹⁸; P{sns-mCherry.NLS}attP2 e¹ mbc^D11.2/TM3, Sb¹ Ser¹

92195

P{GawB}kirre^rP298-G4; e^s mbc^D11.2/TM3, P{HZR+6.8Xb}JG1, Sb¹ Ser¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

D11.2

(Balagopalan et al., 2006)

Mbc^D11.2

(Ishimaru et al., 2004)

mbc^D11.2

(Toret et al., 2018, Haralalka et al., 2014, Rudolf et al., 2014, Bonn et al., 2013, Biersmith et al., 2011, Haralalka et al., 2011, Bulchand et al., 2010, Shelton et al., 2009, Massarwa et al., 2007, Balagopalan et al., 2006, Menon et al., 2005, Paladi and Tepass, 2004)

mbc^D112

(Rudolf et al., 2014)

mbc^Dll.2

(Gallio et al., 2004)

mbcD11.2

(Geisbrecht et al., 2008)

Name Synonyms

Secondary FlyBase IDs

References (23)

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