FlyBase Allele Report: Dmel\Pvr[λ.UASp.Tag:MYC]

General Information

Symbol

Dmel\Pvr^{λ.UASp.Tag:MYC}

Species

D. melanogaster

Name

FlyBase ID

FBal0127283

Feature type

allele

Associated gene

Dmel\Pvr

Associated Insertion(s)

Carried in Construct

P{UASp-Pvr.λ}

Also Known As

UAS-λpvr, UAS-Pvr^act, λ-PVR, UAS-Pvr^CA

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Duchek et al., 2001)

Progenitor genotype

btl^λ.UAS

(Duchek et al., 2001)

Pvr^UAS.cDa

(Duchek et al., 2001)

Carried in construct

P{UASp-Pvr.λ}

Cytology

Description

UASp regulatory sequences drive expression of an activated form of Pvr in which the Pvr extracellular ligand-binding domain has been replaced by the dimerization domain of the bacteriophage λ cI repressor (to induce constitutive dimerization of the encoded protein). A Tag:MYC epitope has been inserted into the BglII site at amino acid residue 785, N-terminal of the transmembrane domain.

(Duchek et al., 2001)

Allele components

Component

Use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

Pvr

Tagged with

Tag:MYC

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of cancer

CEA

(Wang et al., 2023, Lodge et al., 2021)

model of carcinoma

CEA

(Bawa et al., 2020)

model of nephrolithiasis

CEA

(Xu et al., 2024)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of carcinoma

is ameliorated by tn^ΔA

(Bawa et al., 2020)

model of cancer

is ameliorated by kuz^UAS.cFa

(Wang et al., 2023)

is ameliorated by N^intra.GS.UAS

(Wang et al., 2023)

model of nephrolithiasis

is ameliorated by Jra^JF01184

(Xu et al., 2024)

is ameliorated by Uro^{UAS.127D01-tag}

(Xu et al., 2024)

is ameliorated by bsk^DN.UAS

(Xu et al., 2024)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell migration | larval stage, with Scer\GAL4^repo

(Witte et al., 2009)

abnormal cell shape | larval stage, with Scer\GAL4^lz-gal4

(Wang et al., 2023)

abnormal developmental rate, with Scer\GAL4^GS.spok

(Pan and O'Connor, 2021)

abnormal developmental rate, with Scer\GAL4^phtm.PO

(Pan and O'Connor, 2021)

abnormal neuroanatomy | larval stage, with Scer\GAL4^repo

(Witte et al., 2009)

decreased body size | P-stage, with Scer\GAL4^spok.1.45

(Pan and O'Connor, 2021)

increased body size | adult stage | nutrition conditional, with Scer\GAL4^Hml.Δ

(P et al., 2020)

increased cell number | larval stage, with Scer\GAL4^He.PZ

(Zettervall et al., 2004)

increased cell number | larval stage, with Scer\GAL4^Hml.Δ

(P et al., 2020)

increased size | adult stage | nutrition conditional, with Scer\GAL4^Hml.Δ

(P et al., 2020)

increased size | larval stage, with Scer\GAL4^spok.1.45

(Pan and O'Connor, 2021)

neoplasia | larval stage, with Scer\GAL4^Bx-MS1096

(Tran et al., 2013)

neoplasia | third instar larval stage, with Scer\GAL4^dpp.blk1

(Bawa et al., 2020)

partially lethal, with Scer\GAL4^spok.1.45

(Pan and O'Connor, 2021)

viable, with Scer\GAL4^srp.Hemo

(Bruckner et al., 2004)

Phenotype Manifest In

actin filament | ectopic, with Scer\GAL4^slbo.2.6

(Somogyi and Rorth, 2004)

Bolwig nerve, with Scer\GAL4^repo

(Witte et al., 2009)

border follicle cell, with Scer\GAL4^slbo.2.6

(Jekely et al., 2005)

centripetal follicle cell, with Scer\GAL4^slbo.2.6

(Duchek et al., 2001)

centripetally migrating follicle cell & actin filament, with Scer\GAL4^slbo.2.6

(Duchek et al., 2001)

embryonic/first instar larval cuticle, with Scer\GAL4^69B

(Rosin et al., 2004)

embryonic/larval crystal cell | larval stage, with Scer\GAL4^lz-gal4

(Wang et al., 2023)

embryonic/larval glial cell, with Scer\GAL4^repo

(Witte et al., 2009)

embryonic/larval hemocyte, with Scer\GAL4^srp.Hemo

(Bruckner et al., 2004)

embryonic/larval hemocyte | increased number | larval stage, with Scer\GAL4^Hml.Δ

(P et al., 2020)

follicle cell & actin filament | somatic clone, with Scer\GAL4^Act5C.PI

(Duchek et al., 2001)

follicle cell | somatic clone, with Scer\GAL4^Act5C.PI

(Duchek et al., 2001)

hemocyte, with Scer\GAL4^He.PZ

(Zettervall et al., 2004)

hemocyte | larval stage, with Scer\GAL4^He.PZ

(Tran et al., 2013)

lamellocyte, with Scer\GAL4^He.PZ

(Zettervall et al., 2004)

melanotic mass, with Scer\GAL4^He.PZ

(Zettervall et al., 2004)

prothoracic gland | larval stage, with Scer\GAL4^spok.1.45

(Pan and O'Connor, 2021)

wing | nutrition conditional, with Scer\GAL4^Hml.Δ

(P et al., 2020)

wing disc, with Scer\GAL4^dpp.blk1

(Wang et al., 2023)

wing disc | larval stage, with Scer\GAL4^Bx-MS1096

(Tran et al., 2013)

wing disc | third instar larval stage, with Scer\GAL4^dpp.blk1

(Bawa et al., 2020)

Detailed Description

Statement

Reference

Third instar larvae expressing Pvr^{λ.UASp.Tag:MYC} under the control of Scer\GAL4^dpp.blk1 show bigger wing discs.

(Bawa et al., 2020)

Scer\GAL4^He.PZ-mediated expression of Pvr^{λ.Scer\UAS.T:Hsap\MYC} increases hemocyte counts in larvae.

Scer\GAL4^Bx-MS1096-mediated expression of Pvr^{λ.Scer\UAS.T:Hsap\MYC} induces hypertrophic tumor-like structures.

(Tran et al., 2013)

Expression of Pvr^{λ.UASp.Tag:MYC} in larvae under the control of Scer\GAL4^repo results in overmigration of glial cells along the Bolwig nerve compared to controls.

(Witte et al., 2009)

Migration of border cells is severely impaired in Pvr^{λ.Scer\UAS.T:Hsap\MYC}; Scer\GAL4^slbo.2.6 animals.

(Jekely et al., 2005)

Hemocytes in Pvr^{λ.Scer\UAS.T:Hsap\MYC}; Scer\GAL4^srp.Hemo late embryos do not exhibit abnormal aggregation.

(Bruckner et al., 2004)

Hemocytes in Pvr^{λ.Scer\UAS.T:Hsap\MYC}; Scer\GAL4^srp.Hemo late stage embryos aggregate abnormally, but a low percentage have a mild deficiency of hemocytes in the ventral-posterior region and in the posterior end of the elongated germ band. These animals are viable.

(Bruckner et al., 2004)

The polarity of the epithelia in Pvr^{λ.Scer\UAS.T:Hsap\MYC}; Scer\GAL4^69B embryos is normal. The cuticles made by these embryos are normal apart from holes at the termini. The organization of filamentous actin in the eye discs of Pvr^{λ.Scer\UAS.T:Hsap\MYC}; Scer\GAL4^GMR.PF larvae is normal.

(Rosin et al., 2004)

When Pvr^{λ.Scer\UAS.T:Hsap\MYC} is driven by Scer\GAL4^slbo.2.6 in the follicle cells, an increase is seen in the accumulation of F-actin. These cells also show a disruption of the normal cell shape.

(Somogyi and Rorth, 2004)

When expression is driven by Scer\GAL4^He.PZ, hemocyte proliferation and lamellocyte numbers are increased, and crystal cells are unaffected. Melanotic masses form.

(Zettervall et al., 2004)

When clones of Pvr^{λ.Scer\UAS.T:Hsap\MYC} expressing cells (driven by Scer\GAL4^Act5C.PI) are made in the follicle cells, massive F-actin accumulation, actin rich extensions and changes in cell shape are seen. When Pvr^{λ.Scer\UAS.T:Hsap\MYC} is driven by Scer\GAL4^slbo.2.6, causes disruption in centripetal cell morphology and abnormal actin accumulation.

(Duchek et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Pvr^{λ.UASp.Tag:MYC}, Scer\GAL4^lz-gal4 has abnormal cell shape | larval stage phenotype, suppressible by hep^RNAi.UAS, Scer\GAL4^lz-gal4

(Wang et al., 2023)

Pvr^{λ.UASp.Tag:MYC}, Scer\GAL4^lz-gal4 has abnormal cell shape | larval stage phenotype, suppressible by kuz^UAS.cFa, Scer\GAL4^lz-gal4

(Wang et al., 2023)

Pvr^{λ.UASp.Tag:MYC}, Scer\GAL4^phtm.PO has abnormal developmental rate phenotype, suppressible | partially by Stat92E^HMS00035, Scer\GAL4^phtm.PO

(Pan and O'Connor, 2021)

Pvr^{λ.UASp.Tag:MYC}, Scer\GAL4^dpp.blk1 has neoplasia | third instar larval stage phenotype, suppressible | partially by tn^ΔA/tn^ΔA

(Bawa et al., 2020)

Phenotype Manifest In

Suppressed by

Statement

Reference

Pvr^{λ.UASp.Tag:MYC}, Scer\GAL4^lz-gal4 has embryonic/larval crystal cell | larval stage phenotype, suppressible by hep^RNAi.UAS, Scer\GAL4^lz-gal4

(Wang et al., 2023)

Pvr^{λ.UASp.Tag:MYC}, Scer\GAL4^lz-gal4 has embryonic/larval crystal cell | larval stage phenotype, suppressible by kuz^UAS.cFa, Scer\GAL4^lz-gal4

(Wang et al., 2023)

Pvr^{λ.UASp.Tag:MYC}, Scer\GAL4^dpp.blk1 has wing disc phenotype, suppressible by N^intra.GS.UAS, Scer\GAL4^dpp.blk1

(Wang et al., 2023)

Pvr^{λ.UASp.Tag:MYC}, Scer\GAL4^dpp.blk1 has wing disc | third instar larval stage phenotype, suppressible | partially by tn^ΔA/tn^ΔA

(Bawa et al., 2020)

Pvr^{λ.UASp.Tag:MYC}, Scer\GAL4^slbo.2.6 has actin filament | ectopic phenotype, suppressible | partially by Cortactin^unspecified

(Somogyi and Rorth, 2004)

Pvr^{λ.UASp.Tag:MYC}, Scer\GAL4^slbo.2.6 has centripetally migrating follicle cell & actin filament phenotype, suppressible by mbc^D11.2

(Duchek et al., 2001)

Pvr^{λ.UASp.Tag:MYC}, Scer\GAL4^slbo.2.6 has centripetal follicle cell phenotype, suppressible by mbc^D11.2

(Duchek et al., 2001)

NOT suppressed by

Statement

Reference

Pvr^{λ.UASp.Tag:MYC}, Scer\GAL4^dpp.blk1 has wing disc phenotype, non-suppressible by N^RNAi.UAS.cUa, Scer\GAL4^dpp.blk1

(Wang et al., 2023)

Pvr^{λ.UASp.Tag:MYC}, Scer\GAL4^dpp.blk1 has wing disc phenotype, non-suppressible by bsk^K53R.UAS, Scer\GAL4^dpp.blk1

(Wang et al., 2023)

Pvr^{λ.UASp.Tag:MYC}, Scer\GAL4^dpp.blk1 has wing disc phenotype, non-suppressible by hep^RNAi.UAS, Scer\GAL4^dpp.blk1

(Wang et al., 2023)

Additional Comments

Genetic Interactions

Statement

Reference

When Pvr^{λ.Scer\UAS.T:Hsap\MYC} is driven by Scer\GAL4^slbo.2.6 in a homozygous Cortactin^unspecified background, a small but significant suppression is seen in the F-Actin phenotype.

(Somogyi and Rorth, 2004)

When mbc^D11.2 mutant somatic clones are made in border cells in addition to Pvr^{λ.Scer\UAS.T:Hsap\MYC}, driven by Scer\GAL4^slbo.2.6, a strong attenuation of the Pvr^{λ.Scer\UAS.T:Hsap\MYC} phenotype is seen.

(Duchek et al., 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

Pvr^{λ.UASp.Tag:MYC}/Scer\GAL4^srp.Hemo partially rescues Pvr¹

(Bruckner et al., 2004)

Comments

Pvr^{λ.Scer\UAS.T:Hsap\MYC}; Scer\GAL4^srp.Hemo rescues the macrophage aggregation phenotype of stage 16 Pvr¹ homozygous embryos. However, these embryos retain a mild deficiency of hemocytes in the ventral-posterior region and in the posterior end of the elongated germ band.

(Bruckner et al., 2004)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

58428

w¹¹¹⁸; P{UASp-Pvr.λ}mP1

58496

w¹¹¹⁸; P{UASp-Pvr.λ}mP10

Notes on Origin

Discoverer