dati^KG02689 females are courted normally but fail to accept male courtship and very rarely mate. They usually stand still for long periods of time, but when courted by males, they can flee at considerable speed. In addition, when cornered by a courting male, they engage in a series of rejection behaviours that include kicking and curling their abdomen.

dati^KG02689 females perform poorly in 'negative geotaxis' climbing assays.

The alpha and beta lobes of the mushroom body in dati^KG02689 mutants are wild type, but the gamma lobes are malformed with a generally withered appearance and have significantly different curvature.

Clonal analysis using the 'FYT system' demonstrates that females fail to accept male mating when brain regions 'AntB2', 'PosA3' or 'PosC4' are mutant for dati^KG02689.

dati^KG02689 homozygotes exhibit severe abnormalities in the cholinergic tracts of the antennal lobes. The population of dorsal lateral neurons in the region 'AntB2' are either reduced or transformed to cholinergic neuronal types with an abnormal morphology. These transformations affect several glomeruli, including DA1.

T-Maze tests, in which flies are tested for moving away or towards an aversive odor, indicate that olfactory behaviour is impaired in dati^KG02689 mutants.

In the lateral horn of dati^KG02689 mutants, there is a reduction of approximately 10% of the lateral horn neuropile area and the cholinergic projections from the antennal lobe towards the lateral horn are also affected. Like in the antennal lobe, there are larger neurons in the lateral horn which are not present in the wild type. Furthermore, there are more CHA-positive cells around the lateral horn.