FB2024_03 , released June 25, 2024
Allele: Dmel\HSPC300Δ54.3
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General Information
Symbol
Dmel\HSPC300Δ54.3
Species
D. melanogaster
Name
FlyBase ID
FBal0220485
Feature type
allele
Associated gene
Dmel\HSPC300
Associated Insertion(s)
?{}HSPC300-Δ54.3
P{EP}EP506
Carried in Construct
Key Links
Genomic Maps

Allele class

loss of function allele

Mutagen
Nature of the Allele
Allele class

loss of function allele

(Qurashi et al., 2007)
Mutagen
P-element activity
(Qurashi et al., 2007)
Progenitor genotype
P{EP}EP506
(Qurashi et al., 2007)
Associated Insertion(s)
?{}HSPC300-Δ54.3
P{EP}EP506
Cytology
Description

The progenitor P{EP}HSPC300EP506 insertion remains on the chromosome. The 3' end of the insertion is flanked by a stretch of 208bp of HSPC300 sequences followed by an insertion of 208bp of unrelated sequences into the HSPC300 intron.

(Qurashi et al., 2007)
Allele components
Component
Use(s)
Inserted element
P{EP}
Regulatory region(s)
UAS
Mutations Mapped to the Genome
Curation Data
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Homozygotes die at the late pupal stage.

      Homozygous embryos (lacking zygotic HSPC300 function) have normal central nervous system axon morphology.

      Homozygous embryos derived from homozygous female germline clones (lacking zygotic and maternal HSPC300 function) show severe, though variable, nervous system defects ranging from broken and disorganised longitudinal connectives and commissures to remnants of axons or depletion of all central nervous system axon bundles. In addition, lethality is shifted to embryonic stages in these animals and the embryos appear overall disorganised.

      HSPC300EP506/HSPC300Δ54.3 animals show 30% viability.

      Zygotic null, maternal hypomorph embryos (derived from HSPC300EP506/HSPC300Δ54.3 females mated to HSPC300Δ54.3/+ males) sometimes show abnormal crossing of the midline by axons (10% of embryos), and in the most severe cases, axons ectopically cross the midline several times. Commissures and longitudinal connectives are not properly formed.

      The neuromuscular junctions of homozygous larvae are severely reduced in length (to 66% of wild type) and have supernumerary buds (88% increase compared to wild type). Heterozygous larvae also show a significant reduction in synaptic length compared to wild type.

      Homozygous pharate adults occasionally have bent thoracic bristles, although the number of bristles on the head and thorax are normal.

      (Qurashi et al., 2007)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      HSPC300Δ54.3
      (Qurashi et al., 2007)
      hspc300Δ54.3
      (Chaudhari et al., 2021)
      Name Synonyms
      Secondary FlyBase IDs
        References (2)