FlyBase Allele Report: Dmel\Lamtor3[RNAi.UASp]

General Information

Symbol

Dmel\Lamtor3^RNAi.UASp

Species

D. melanogaster

Name

FlyBase ID

FBal0220794

Feature type

allele

Associated gene

Dmel\Lamtor3

Associated Insertion(s)

Carried in Construct

P{UASp-Lamtor3.RNAi}

Key Links

Transgenic product class

RNAi_reagent

(Mouchel-Vielh et al., 2008)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Mouchel-Vielh et al., 2008)

Mutagen

in vitro construct

(Mouchel-Vielh et al., 2008)

Progenitor genotype

Carried in construct

P{UASp-Lamtor3.RNAi}

Cytology

Description

UASp regulatory sequences drive expression of a head-to-head inverted repeat of a 499bp fragment that contains the entire CG5110 coding region.

(Mouchel-Vielh et al., 2008)

Allele components

Component

Use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

Lamtor3

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Df(2L)ED1175, Scer\GAL4^sd-SG29.1

(Mouchel-Vielh et al., 2008)

visible, with Scer\GAL4^ap-md544

(Mouchel-Vielh et al., 2008)

visible, with Scer\GAL4^en-e16E

(Mouchel-Vielh et al., 2008)

visible, with Scer\GAL4^sd-SG29.1

(Mouchel-Vielh et al., 2008)

visible | adult stage, with Scer\GAL4^sd.PU

(Mouchel-Vielh et al., 2011)

visible | heat sensitive, with Scer\GAL4^hs.PB

(Mouchel-Vielh et al., 2008)

Phenotype Manifest In

wing, with Scer\GAL4^ap-md544

(Mouchel-Vielh et al., 2008)

wing vein | ectopic, with Df(2L)ED1175, Scer\GAL4^sd-SG29.1

(Mouchel-Vielh et al., 2008)

wing vein | ectopic, with Scer\GAL4^ap-md544

(Mouchel-Vielh et al., 2008)

wing vein | ectopic, with Scer\GAL4^en-e16E

(Mouchel-Vielh et al., 2008)

wing vein | ectopic, with Scer\GAL4^sd.PU

(Mouchel-Vielh et al., 2011)

wing vein | ectopic, with Scer\GAL4^sd-SG29.1

(Mouchel-Vielh et al., 2008)

wing vein | ectopic | heat sensitive, with Scer\GAL4^hs.PB

(Mouchel-Vielh et al., 2008)

Detailed Description

Statement

Reference

Expression of CG5110^dsRNA.UASp under the control of Scer\GAL4^sd.PU leads to ectopic wing veins.

(Mouchel-Vielh et al., 2011)

Expression of CG5110^{dsRNA.Scer\UAS.P\T} under the control of Scer\GAL4^en-e16E, Scer\GAL4^ap-md544 or Scer\GAL4^sd-SG29.1 results in an ectopic wing vein phenotype. Expression of CG5110^{dsRNA.Scer\UAS.P\T} under the control of Scer\GAL4^ap-md544 results in a ballooned wing phenotype.

The penetrance of the ectopic wing phenotype caused by expression of CG5110^{dsRNA.Scer\UAS.P\T} under the control of Scer\GAL4^sd-SG29.1 is increased if the flies are also carrying Df(2L)ED1175.

Ectopic wing veins are formed in 69.5% of females when CG5110^{dsRNA.Scer\UAS.P\T} is expressed under the control of Scer\GAL4^hs.PB using heat shock at 129 hours after egg laying (early pupal stage).

(Mouchel-Vielh et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Lamtor3^RNAi.UASp, Scer\GAL4^sd.PU has visible | adult stage phenotype, enhanceable by corto[+]/corto⁴²⁰

(Mouchel-Vielh et al., 2011)

Lamtor3^RNAi.UASp, Scer\GAL4^sd.PU has visible | adult stage phenotype, enhanceable by corto^07128b/corto[+]

(Mouchel-Vielh et al., 2011)

Other

Statement

Reference

Lamtor3^RNAi.UASp, Scer\GAL4^sd-SG29.1, rl^UAS.cKa has visible phenotype

(Mouchel-Vielh et al., 2008)

Phenotype Manifest In

Enhanced by

Statement

Reference

Lamtor3^RNAi.UASp, Scer\GAL4^sd.PU has wing vein | ectopic phenotype, enhanceable by corto[+]/corto⁴²⁰

(Mouchel-Vielh et al., 2011)

Lamtor3^RNAi.UASp, Scer\GAL4^sd.PU has wing vein | ectopic phenotype, enhanceable by corto^07128b/corto[+]

(Mouchel-Vielh et al., 2011)

Other

Statement

Reference

Lamtor3^RNAi.UASp, Scer\GAL4^sd-SG29.1, rl^UAS.cKa has wing phenotype

(Mouchel-Vielh et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

Either corto⁴²⁰/+ or corto^07128b/+ enhances the ectopic wing vein phenotype induced by expression of CG5110^dsRNA.UASp under the control of Scer\GAL4^sd.PU.

(Mouchel-Vielh et al., 2011)

100% of flies co-expressing rl^Scer\UAS.cKa and CG5110^{dsRNA.Scer\UAS.P\T} under the control of Scer\GAL4^sd-SG29.1 have an ectopic wing vein phenotype and in addition, 19.4% of them also have a blistered wing phenotype.

(Mouchel-Vielh et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

CG5110^{dsRNA.Scer\UAS.P\T}

CG5110^dsRNA.UASp

Lamtor3^RNAi.UASp

Lamtor3^dsRNA.UASp

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

Open Close