FB2024_04 , released June 25, 2024
Allele: Dmel\Gem3ΔN.UAS
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General Information
Symbol
Dmel\Gem3ΔN.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0220955
Feature type
allele
Associated gene
Dmel\Gem3
Associated Insertion(s)
Carried in Construct
P{UAS-Gem3.ΔN}
Also Known As
Gem3BART, UAS.Gem3BART
Key Links
Transgenic product class
inframe_deletion
(Cauchi et al., 2008)
Mutagen
Nature of the Allele
Transgenic product class
inframe_deletion
(Cauchi et al., 2008)
Mutagen
in vitro construct
(Cauchi et al., 2008)
Progenitor genotype
Carried in construct
P{UAS-Gem3.ΔN}
Cytology
Description

UASt regulatory sequences drive expression of the C-terminus of Gem3 (the N-terminal 424 amino acid residues are missing).

(Cauchi et al., 2008)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Gem3
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of one copy of Gem3ΔN.Scer\UAS driven by Scer\GAL4Mef2.PR has no effect on both motor function and survival throughout adulthood; expression of two copies of Gem3ΔN.Scer\UAS results in the majority of flies being flightless (5 days old) and flight ability progressively declines (none fly by day 15) and there is a progressive drop in adult survival.

      (Borg et al., 2015)

      Flies expressing Gem3ΔN.Scer\UAS (co-expressed with Dcr-2Scer\UAS.cDa) under the control of Scer\GAL4Mef2.PR show an age-dependent reduction in climbing ability compared to controls. The majority of flies are flightless at 5 days post-eclosion.

      Flies co-expressing Gem3Scer\UAS.cCa, Gem3GD17221 and Gem3ΔN.Scer\UAS under the control of Scer\GAL4Mef2.PR are flight defective.

      (Borg and Cauchi, 2013)

      Animals expressing Gem3ΔN.Scer\UAS under the control of Scer\GAL4how-24B show several defects at metamorphosis; the flies are "headless" as a results of failed head eversion and the wings are shortened. The pupae are elongated compared to wild type. The abdomen remains elongated (as in the larval stage) during pupal development in these animals, although its shows normal segmentation as shown by the complete bristle pattern on the tergites. The leg discs evert but the legs are not fully elongated.

      Eclosed flies expressing Gem3ΔN.Scer\UAS under the control of Scer\GAL4Mef2.PR have a reduced ability to fly, although they can walk and jump normally. The indirect flight muscles in these animals are shrunken, resulting in increased spacing, and breakages and large tears can be seen between the muscle fibres.

      (Cauchi et al., 2008)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT suppressed by
      Statement
      Reference

      Gem3ΔN.UAS, Scer\GAL4Mef2.PR has flightless phenotype, non-suppressible by SmnUASp.Venus, Scer\GAL4Mef2.PR

      (Borg and Cauchi, 2013)
      Enhancer of
      Statement
      Reference

      Gem3ΔN.UAS, Scer\GAL4Mef2.PR is an enhancer of partially lethal phenotype of Scer\GAL4Mef2.PR, iclnUAS.cBa

      (Borg et al., 2016)
      Suppressor of
      Statement
      Reference

      Gem3ΔN.UAS, Scer\GAL4Mef2.PR is a suppressor of flightless phenotype of Scer\GAL4Mef2.PR, iclnUAS.cBa

      (Borg et al., 2016)
      Other
      Statement
      Reference

      Gem2UAS.cBa, Gem3ΔN.UAS, Scer\GAL4Mef2.PR has abnormal locomotor behavior | third instar larval stage phenotype

      (Lanfranco et al., 2017)

      Gem3ΔN.UAS, Scer\GAL4Mef2.PR, SmnRNAi.C.UAS.WIZ has abnormal size | pupal stage phenotype

      (Lanfranco et al., 2017)

      Gem3ΔN.UAS, Scer\GAL4Mef2.PR, SmnUAS.Tag:FLAG has abnormal locomotor behavior | third instar larval stage phenotype

      (Lanfranco et al., 2017)

      Gem3ΔN.UAS, Scer\GAL4Mef2.PR, SmnUAS.Tag:FLAG has abnormal size | pupal stage phenotype

      (Lanfranco et al., 2017)

      +/Df(1)ED6716, Gem3ΔN.UAS, Scer\GAL4Mef2.PR has short lived phenotype

      (Lanfranco et al., 2017)

      +/Df(1)ED6716, Gem3ΔN.UAS, Scer\GAL4Mef2.PR has abnormal locomotor behavior | adult stage phenotype

      (Lanfranco et al., 2017)

      Gem3ΔN.UAS, GlosGD17786, Scer\GAL4Mef2.PR has abnormal locomotor behavior | third instar larval stage phenotype

      (Lanfranco et al., 2017)

      Gem3ΔN.UAS, GlosGD17786, Scer\GAL4Mef2.PR has abnormal size | pupal stage phenotype

      (Lanfranco et al., 2017)

      Gem3ΔN.UAS, GlosGD17786, Scer\GAL4Mef2.PR has lethal - all die before end of P-stage phenotype

      (Lanfranco et al., 2017)

      Gem3ΔN.UAS, GlosGD17786, Scer\GAL4elav-C155 has partially lethal - majority die phenotype

      (Lanfranco et al., 2017)

      Gem3ΔN.UAS, Scer\GAL4elav-C155, rigNIG.11171R has lethal phenotype

      (Lanfranco et al., 2017)

      Gem2UAS.cBa, Gem3ΔN.UAS, Scer\GAL4Mef2.PR has abnormal size | pupal stage phenotype

      (Lanfranco et al., 2017)

      Gem2UAS.cBa, Gem3ΔN.UAS, Scer\GAL4elav-C155 has partially lethal - majority die phenotype

      (Lanfranco et al., 2017)

      Gem3ΔN.UAS, Scer\GAL4elav-C155, SmnRNAi.C.UAS.WIZ has partially lethal - majority die phenotype

      (Lanfranco et al., 2017)

      Gem3ΔN.UAS, Scer\GAL4elav-C155, SmnUAS.Tag:FLAG has partially lethal - majority die phenotype

      (Lanfranco et al., 2017)

      Gem3ΔN.UAS, Scer\GAL4Mef2.PR, VletGD5216 has short lived phenotype

      (Lanfranco et al., 2017)

      Gem3ΔN.UAS, Scer\GAL4Mef2.PR, rigNIG.11171R has abnormal locomotor behavior | third instar larval stage phenotype

      (Lanfranco et al., 2017)

      Gem3ΔN.UAS, Scer\GAL4Mef2.PR, rigNIG.11171R has abnormal size | pupal stage phenotype

      (Lanfranco et al., 2017)

      Gem3ΔN.UAS, Scer\GAL4Mef2.PR, rigUAS.ECFP has abnormal locomotor behavior | third instar larval stage phenotype

      (Lanfranco et al., 2017)

      Gem3ΔN.UAS, Scer\GAL4Mef2.PR, rigUAS.ECFP has abnormal size | pupal stage phenotype

      (Lanfranco et al., 2017)

      Gem3ΔN.UAS, Scer\GAL4Mef2.PR, SmnRNAi.C.UAS.WIZ has abnormal locomotor behavior | third instar larval stage phenotype

      (Lanfranco et al., 2017)

      Df(3R)Exel6178, Gem3ΔN.UAS, Scer\GAL4Mef2.PR has abnormal flight | progressive phenotype

      (Borg et al., 2016)

      Gem3ΔN.UAS, Scer\GAL4Mef2.PR, Tgs1GD14932 has abnormal flight | progressive phenotype

      (Borg et al., 2016)

      Gem3ΔN.UAS, Scer\GAL4Mef2.PR, SmnRNAi.FL.UAS.WIZ has lethal phenotype

      (Borg et al., 2015)

      Gem3ΔN.UAS, Scer\GAL4Mef2.PR, SmnRNAi.N.UAS.WIZ has lethal phenotype

      (Borg et al., 2015)

      Gem3ΔN.UAS, Scer\GAL4Mef2.PR, SmnRNAi.C.UAS.WIZ has short lived phenotype

      (Borg et al., 2015)

      Gem3ΔN.UAS, Scer\GAL4Mef2.PR, SmnRNAi.C.UAS.WIZ has abnormal flight phenotype

      (Borg et al., 2015)

      Gem2NIG.10419R, Gem3ΔN.UAS, Scer\GAL4Mef2.PR has abnormal flight phenotype

      (Borg et al., 2015)

      Gem2NIG.10419R, Gem3ΔN.UAS, Scer\GAL4Mef2.PR has partially lethal phenotype

      (Borg et al., 2015)

      Gem2NIG.10419R, Gem3ΔN.UAS, Scer\GAL4Mef2.PR has lethal | conditional phenotype

      (Borg et al., 2015)

      Df(2R)exu1, Gem3ΔN.UAS, Scer\GAL4Mef2.PR has short lived phenotype

      (Borg et al., 2015)

      Gem2UAS.cBa, Gem3ΔN.UAS, Scer\GAL4Mef2.PR has lethal phenotype

      (Borg et al., 2015)

      Gem3ΔN.UAS, Scer\GAL4Mef2.PR, rigNIG.11171R has lethal phenotype

      (Borg et al., 2015)

      Gem3ΔN.UAS, Scer\GAL4Mef2.PR, rigUAS.ECFP has flightless phenotype

      (Borg et al., 2015)

      Gem3ΔN.UAS, Scer\GAL4Mef2.PR, rigUAS.ECFP has short lived phenotype

      (Borg et al., 2015)

      Gem3ΔN.UAS, Scer\GAL4Mef2.PR, rigΔN.UAS has flightless phenotype

      (Borg et al., 2015)

      Gem3ΔN.UAS, Scer\GAL4Mef2.PR, rigΔN.UAS has short lived phenotype

      (Borg et al., 2015)

      Gem3ΔN.UAS, Scer\GAL4Mef2.PR, SmnUAS.GFP has lethal phenotype

      (Borg et al., 2015)

      Gem3ΔN.UAS, Scer\GAL4Mef2.PR, SmnUAS.Tag:FLAG has abnormal flight phenotype

      (Borg et al., 2015)

      Gem3ΔN.UAS, Scer\GAL4Mef2.PR, SmnUAS.Tag:FLAG has partially lethal phenotype

      (Borg et al., 2015)

      Df(2R)exu1, Gem3ΔN.UAS, Scer\GAL4Mef2.PR has flightless | progressive phenotype

      (Borg et al., 2015)

      Gem2ΔC.UAS, Gem3ΔN.UAS, Scer\GAL4Mef2.PR has abnormal flight | progressive phenotype

      (Borg et al., 2015)

      Gem2ΔN.UAS, Gem3ΔN.UAS, Scer\GAL4Mef2.PR has lethal phenotype

      (Borg et al., 2015)

      Gem3ΔN.UAS, Scer\GAL4Mef2.PR, SmnΔ6.UAS.GFP has flightless phenotype

      (Borg et al., 2015)

      Gem3ΔN.UAS, Scer\GAL4Mef2.PR, SmnΔ6.UAS.GFP has short lived phenotype

      (Borg et al., 2015)
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Adult flies bearing Df(1)ED6716 and also expressing Gem3ΔN.Scer\UAS under the control of Scer\GAL4Mef2.PR display rapid age-progressive decrease in viability as well as flight ability relative to controls.

      Expression of Gem3ΔN.Scer\UAS together with GlosGD17786 under the Scer\GAL4Mef2.PR driver are adult lethal and simultaneous expression of Gem3ΔN.Scer\UAS and any of the following: GlosGD17786, rigNIG.11171R, rigScer\UAS.T:Avic\GFP-ECFP, Gem2Scer\UAS.cBa, SmndsRNA.C.Scer\UAS.WIZ or SmnScer\UAS.T:Zzzz\FLAG leads to strongly reduced mobility in third instar larvae and more elongated shape of the puparium.

      Scer\GAL4elav-C155-driven co-expression of Gem3ΔN.Scer\UAS and GlosGD17786, Gem2Scer\UAS.cBa, SmndsRNA.C.Scer\UAS.WIZ or SmnScer\UAS.T:Zzzz\FLAG reduced adult viability and Gem3ΔN.Scer\UAS;rigScer\UAS.T:Avic\GFP-ECFP flies are completely lethal.

      Flies expressing simultaneously VletGD5216 and Gem3ΔN.Scer\UAS under the control of Scer\GAL4Mef2.PR display significantly reduced survival rate compared to controls.

      (Lanfranco et al., 2017)

      Df(3R)Exel6178 adult heterozygotes expressing Gem3ΔN.Scer\UAS under the control of Scer\GAL4Mef2.PR display age-progressive loss of flight ability.

      No decline in adult flight ability compared to controls is observed when wmdKK100974 and Gem3ΔN.Scer\UAS are co-expressed under the Scer\GAL4Mef2.PR driver.

      Expression of Tgs1GD14932 together with Gem3ΔN.Scer\UAS under the control of Scer\GAL4Mef2.PR leads to age-progressive flight defects.

      Flies expressing both iclnScer\UAS.cBa and Gem3ΔN.Scer\UAS under the control of Scer\GAL4Mef2.PR are completely lethal.

      (Borg et al., 2016)

      Co-expression of Gem3ΔN.Scer\UAS and rigScer\UAS.T:Avic\GFP-ECFP or rigΔN.Scer\UAS driven by Scer\GAL4Mef2.PR results in flightless flies with shortened lifespans (compared to either single expression, which is similar to controls).

      Co-expression of rigNIG.11171R or SmnScer\UAS.T:Avic\GFP or SmndsRNA.N.Scer\UAS.WIZ or SmndsRNA.FL.Scer\UAS.WIZ or Gem2ΔN.Scer\UAS or Gem2Scer\UAS.cBa and Gem3ΔN.Scer\UAS driven by Scer\GAL4Mef2.PR results in lethality.

      Expression of one copy of Gem3ΔN.Scer\UAS driven by Scer\GAL4Mef2.PR in a Df(2R)exu1/+ background results in progressive flight defects and reduced adult viability. Presence of SmnX7 or Df(3L)ED4782 does not result in significant progressive flight or survival phenotypes in flies with expression of Gem3ΔN.Scer\UAS driven by Scer\GAL4Mef2.PR.

      Co-expression of SmnΔ6.Scer\UAS.T:Avic\GFP and Gem3ΔN.Scer\UAS driven by Scer\GAL4Mef2.PR results in flightless flies and a significant drop in adult viability throughout adulthood. Co-expression of SmnScer\UAS.T:Zzzz\FLAG and Gem3ΔN.Scer\UAS driven by Scer\GAL4Mef2.PR results in partial lethality, with the few escapers exhibiting severe flight defects. Co-expression of Gem3ΔN.Scer\UAS and SmndsRNA.C.Scer\UAS.WIZ driven by Scer\GAL4Mef2.PR results in flight defects and reduces adult viability, compared to controls.

      Co-expression of Gem3ΔN.Scer\UAS and Gem2ΔC.Scer\UAS driven by Scer\GAL4Mef2.PR results in progressive age-dependent flight defects (no significant effect on survival with age progression). Co-expression of Gem3ΔN.Scer\UAS and one copy of Gem2NIG.10419R driven by Scer\GAL4Mef2.PR results in flight defects and partial lethality; co-expression of Gem3ΔN.Scer\UAS and two copies of Gem2NIG.10419R driven by Scer\GAL4Mef2.PR results in lethality.

      (Borg et al., 2015)

      Expression of SmnScer\UAS.T:Avic\GFP-YFP.Venus is unable to suppress the flight defects seen when Gem3ΔN.Scer\UAS is expressed under the control of Scer\GAL4Mef2.PR.

      (Borg and Cauchi, 2013)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      Gem3ΔN.UAS is rescued by Scer\GAL4Mef2.PR/Gem3UAS.cCa

      (Borg and Cauchi, 2013)

      Gem3ΔN.UAS is rescued by Scer\GAL4Act5C.PU/Gem3UAS.cCa

      (Cauchi et al., 2008)

      Gem3ΔN.UAS is rescued by Scer\GAL4c179/Gem3UAS.cCa

      (Cauchi et al., 2008)

      Gem3ΔN.UAS is rescued by Scer\GAL4how-24B/Gem3UAS.cCa

      (Cauchi et al., 2008)

      Gem3ΔN.UAS is rescued by Gem3UAS.cCa/Scer\GAL4G7

      (Cauchi et al., 2008)

      Gem3ΔN.UAS is rescued by Scer\GAL4Mef2.PR/Gem3UAS.cCa

      (Cauchi et al., 2008)

      Gem3ΔN.UAS is rescued by Gem3UAS.cCa/Scer\GAL4C57

      (Cauchi et al., 2008)
      Partially rescued by

      Gem3ΔN.UAS is partially rescued by Scer\GAL4Mef2.PR/Gem3UAS.cCa/Gem3GD17221

      (Borg and Cauchi, 2013)
      Comments

      Expression of Gem3Scer\UAS.cCa rescues the flight defects seen in flies expressing Gem3ΔN.Scer\UAS under the control of Scer\GAL4Mef2.PR.

      Expression of Gem3Scer\UAS.cCa rescues the lethality seen in flies co-expressing Gem3GD17221 and Gem3ΔN.Scer\UAS under the control of Scer\GAL4Mef2.PR. The rescued flies exhibit flight defects.

      Pupae expressing Gem3ΔN.Scer\UAS under the control of Scer\GAL4how-24B have increased axial ratios.

      (Borg and Cauchi, 2013)

      The lethality caused by expression of Gem3ΔN.Scer\UAS under the control of one of Scer\GAL4Act5C.PU, Scer\GAL4c179, Scer\GAL4how-24B, Scer\GAL4G7, Scer\GAL4Mef2.PR or Scer\GAL4C57 is significantly suppressed by co-expression of Gem3Scer\UAS.cCa.

      (Cauchi et al., 2008)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Gem3BART
      (Cacciottolo et al., 2019, Borg et al., 2016, Borg et al., 2015)
      Gem3ΔN.Scer\UAS
      Gem3ΔN.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (6)