FlyBase Allele Report: Dmel\vtd[RNAi.Sym.UAS]

General Information

Symbol

Dmel\vtd^RNAi.Sym.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0221047

Feature type

allele

Associated gene

Dmel\vtd

Associated Insertion(s)

Carried in Construct

P{Sym-UAS-Rad21}

Key Links

Transgenic product class

RNAi_reagent

(Rollins et al., 2004)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Rollins et al., 2004)

Mutagen

in vitro construct

(Rollins et al., 2004)

Progenitor genotype

Carried in construct

P{Sym-UAS-Rad21}

Cytology

Description

Sense-antisense transcription (driven by two convergent arrays of UAS regulatory sequences) of a 686bp fragment of vtd exon 4 (nucleotides 1152-1837, coordinates relative to GenBank accession AF109926) results in double stranded RNA (dsRNA) expression.

(Rollins et al., 2004)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

vtd

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal, with Scer\GAL4^Act5C.PI

(Hallson et al., 2008)

lethal, with Scer\GAL4^Act5C.PI, vtd^80Fh-1

(Hallson et al., 2008)

lethal - all die before end of pupal stage | partially, with Scer\GAL4^Act5C.PI

(Rollins et al., 2004)

some die during pupal stage | partially, with Scer\GAL4^Act5C.PI

(Rollins et al., 2004)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of vtd^{dsRNA.Sym.Scer\UAS} under the control of Scer\GAL4^Act5C.PI results in reduced survival or both male and female adults, with the males being more strongly affected. The lethality is dramatically enhanced if the animals are also heterozygous for vtd^80Fh-1.

Expression of P{Sym-UAS-Rad21} (which carries both vtd^Sym.Scer\UAS and vtd^{a.Sym.Scer\UAS}) under the control of Scer\GAL4^Act5C.PI results in reduced survival or both male and female adults, with the males being more strongly affected. The lethality is dramatically enhanced if the animals are also heterozygous for vtd^80Fh-1.

(Hallson et al., 2008)

Expression of vtd^{dsRNA.Sym.Scer\UAS} under the control of Scer\GAL4^Act5C.PI can result in pupal lethality, depending on the particular P{Sym-UAS-Rad21} insertion line used. No increase in precocious sister chromatid separation in third larval instar brain squashes is seen in these animals.

Expression of P{Sym-UAS-Rad21} (which carries both vtd^Sym.Scer\UAS and vtd^{a.Sym.Scer\UAS}) under the control of Scer\GAL4^Act5C.PI can result in pupal lethality, depending on the particular P{Sym-UAS-Rad21} insertion line used. No increase in precocious sister chromatid separation in third larval instar brain squashes is seen in these animals.

(Rollins et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Nipped-B[+]/Nipped-B⁴⁰⁷, Scer\GAL4^Act5C.PI, vtd^RNAi.Sym.UAS has lethal phenotype

(Rollins et al., 2004)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Nipped-B⁴⁰⁷/+ shows synthetic lethality with the P{Sym-UAS-Rad21}2 insertion line expressed under the control of Scer\GAL4^Act5C.PI (this particular insertion line does not reduce viability when expressed using Scer\GAL4^Act5C.PI at 25[o]C in an otherwise wild-type background).

(Rollins et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Associated with: vtd^{a.Sym.Scer\UAS}.

(Rollins et al., 2004)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

vtd^RNAi.Sym.UAS

vtd^Sym.Scer\UAS

vtd^{dsRNA.Sym.Scer\UAS}

vtd^{dsRNA.Sym.UAS}

Name Synonyms

Secondary FlyBase IDs

FBal0221048

References (3)

Report Sections

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