FlyBase Allele Report: Dmel\SA1[RNAi.Sym.UAS]

General Information

Symbol

Dmel\SA1^RNAi.Sym.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0221162

Feature type

allele

Associated gene

Dmel\SA1

Associated Insertion(s)

Carried in Construct

P{Sym-UAS-SA1}

Key Links

Transgenic product class

RNAi_reagent

(Rollins et al., 2004)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Rollins et al., 2004)

Mutagen

in vitro construct

(Rollins et al., 2004)

Progenitor genotype

Carried in construct

P{Sym-UAS-SA1}

Cytology

Description

Sense-antisense transcription (driven by two convergent arrays of UAS regulatory sequences) of a 700bp fragment of SA (nucleotides 1516-2215, coordinates relative to GenBank accession Y14277) results in double stranded RNA (dsRNA) expression.

(Rollins et al., 2004)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

SA1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die before end of pupal stage | partially, with Scer\GAL4^Act5C.PI

(Rollins et al., 2004)

some die during pupal stage | partially, with Scer\GAL4^Act5C.PI

(Rollins et al., 2004)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of P{Sym-UAS-SA} (which carries both SA^Sym.Scer\UAS and SA^{a.Sym.Scer\UAS}) under the control of Scer\GAL4^Act5C.PI results in pupal lethality, with the degree of lethality depending on the particular P{Sym-UAS-SA} insertion line used. No increase in precocious sister chromatid separation in third larval instar brain squashes is seen in these animals.

Expression of SA^{dsRNA.Sym.Scer\UAS} under the control of Scer\GAL4^Act5C.PI results in pupal lethality, with the degree of lethality depending on the particular P{Sym-UAS-SA} insertion line used. No increase in precocious sister chromatid separation in third larval instar brain squashes is seen in these animals.

(Rollins et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

SA1^RNAi.Sym.UAS/Scer\GAL4^hs.PB is a suppressor of visible phenotype of ct^K

(Rollins et al., 2004)

Phenotype Manifest In

Suppressor of

Statement

Reference

SA1^RNAi.Sym.UAS/Scer\GAL4^hs.PB is a suppressor of wing phenotype of ct^K

(Rollins et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

The severity of the wing nicking phenotype seen in ct^K mutant males is decreased by expression of SA^{dsRNA.Sym.Scer\UAS} under the control of Scer\GAL4^hs.PB.

The severity of the wing nicking phenotype seen in mutant males is decreased by expression of P{Sym-UAS-SA} (which carries both SA^Sym.Scer\UAS and SA^{a.Sym.Scer\UAS}) under the control of Scer\GAL4^hs.PB.

(Rollins et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Associated with: SA^{a.Sym.Scer\UAS}.

(Rollins et al., 2004)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

SA1^RNAi.Sym.UAS

SA1^{dsRNA.Sym.UAS}

SA^Sym.Scer\UAS

SA^{dsRNA.Sym.Scer\UAS}

SA^{dsRNA.Sym.UAS}

Name Synonyms

Secondary FlyBase IDs

FBal0221163

References (2)

Report Sections

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