UAS regulatory sequences drive expression of a constitutively activated form of the Rgl 1 isoform (the Rgl protein has been made constitutively active by being fused to the Tag:PM(hKRAS) membrane localisation sequence consisting of a polybasic motif followed by a CAAX box).
Expression of Rgl1.Scer\UAS.T:Hsap\CAAX under the control of Scer\GAL4GMR.PF results in a few fusions of ommatidia and weakly rough eyes.
Expression of Rgl1.Scer\UAS.T:Hsap\CAAX under the control of Scer\GAL4Bx-MS1096 results in an almost complete absence of wings or wings with an almost complete absence of veins and an overall reduction of wing size.
Rgl1.UAS.Tag:PM(hKRAS), Scer\GAL4sca.PU is a suppressor of visible phenotype of RalaS25N.cMa.UAS, Scer\GAL4sca.PU
Rgl1.UAS.Tag:PM(hKRAS), Scer\GAL4sca.PU is a suppressor of visible phenotype of RalaS25N.cMa.UAS, Ras85DV12.C40.UAS, Scer\GAL4sca.PU
Rgl1.UAS.Tag:PM(hKRAS), Scer\GAL4sca.PU is a suppressor of visible phenotype of RalaS25N.cMa.UAS, Ras85DV12.G37.UAS, Scer\GAL4sca.PU
Rgl1.UAS.Tag:PM(hKRAS), Scer\GAL4sca.PU is a non-suppressor of lethal | embryonic stage phenotype of Rap1V12.cHa.UAS, Scer\GAL4sca.PU
Rgl1.UAS.Tag:PM(hKRAS), Scer\GAL4sca.PU is a suppressor of microchaeta phenotype of RalaS25N.cMa.UAS, Scer\GAL4sca.PU
Rgl1.UAS.Tag:PM(hKRAS), Scer\GAL4sca.PU is a suppressor of macrochaeta phenotype of RalaS25N.cMa.UAS, Scer\GAL4sca.PU
Rgl1.UAS.Tag:PM(hKRAS), Scer\GAL4sca.PU is a suppressor of chaeta phenotype of RalaS25N.cMa.UAS, Ras85DV12.C40.UAS, Scer\GAL4sca.PU
Rgl1.UAS.Tag:PM(hKRAS), Scer\GAL4sca.PU is a suppressor of chaeta phenotype of RalaS25N.cMa.UAS, Ras85DV12.G37.UAS, Scer\GAL4sca.PU
Co-expression of Rgl1.Scer\UAS.T:Hsap\CAAX suppresses the loss of bristle phenotype caused by expression of RalaS25N.cMa.Scer\UAS under the control of Scer\GAL4sca.PU.
The loss of bristle phenotype seen in flies co-expressing Ras85DV12.C40.Scer\UAS and RalaS25N.cMa.Scer\UAS under the control of Scer\GAL4sca.PU is suppressed by co-expression of Rgl1.Scer\UAS.T:Hsap\CAAX.
The loss of bristle phenotype seen in flies co-expressing Ras85DV12.G37.Scer\UAS and RalaS25N.cMa.Scer\UAS under the control of Scer\GAL4sca.PU is largely suppressed by co-expression of Rgl1.Scer\UAS.T:Hsap\CAAX.