UAS regulatory sequences drive expression of a constitutively activated form of the Rgl 2 isoform (the Rgl protein has been made constitutively active by being fused to the Tag:PM(hKRAS) membrane localisation sequence consisting of a polybasic motif followed by a CAAX box).
Expression of Rgl2.Scer\UAS.T:Hsap\CAAX under the control of Scer\GAL4GMR.PF results in a severe eye phenotype, with degeneration of multiple ommatidia and a reduction in eye size.
Expression of Rgl2.Scer\UAS.T:Hsap\CAAX under the control of Scer\GAL4Bx-MS1096 results in an almost complete absence of wings or wings with an almost complete absence of veins and an overall reduction of wing size.
Flies expressing Rgl2.Scer\UAS.T:Hsap\CAAX under the control of Scer\GAL4sca.PU have missing macrochaetae.