FlyBase Allele Report: Dmel\shrb[G5]

General Information

Symbol

Dmel\shrb^G5

Species

D. melanogaster

Name

FlyBase ID

FBal0230153

Feature type

allele

Associated gene

Dmel\shrb

Associated Insertion(s)

Carried in Construct

Also Known As

vps32^G5

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Vaccari et al., 2009, Rusten et al., 2007)

Progenitor genotype

Cytology

Description

shrb^G5 contains a 384bp deletion.

(Vaccari et al., 2009)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2R:9,142,823..9,143,206 [-]

Comment:

384 bp deletion

(Vaccari et al., 2009)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Huntington's disease

modeled by Hsap\HTT^GMR.Q120

(Rusten et al., 2007)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cytokinesis | dominant

(Eikenes et al., 2015, Matias et al., 2015)

abnormal neuroanatomy | P-stage | somatic clone

(Zhang et al., 2014)

increased occurrence of cell division | cell non-autonomous | third instar larval stage

(Shravage et al., 2013)

Phenotype Manifest In

Detailed Description

Statement

Reference

70% of heterozygous female germaria contain 'stem cysts' in which the female germline stem cells (GSCs) are interconnected to several daughter cells (both linear and branched chains of cells are seen).

(Eikenes et al., 2015)

54% of egg chambers have 32 cells in shrb^G5/+ females. 55% of heterozygous germaria contain 'stem cysts' in which the female germline stem cells are interconnected to daughter cells. The cells in these stem cysts share the same cytoplasm.

(Matias et al., 2015)

shrb^G5 mutant clones in late third instar larval eye imaginal discs causes non-autonomous overproliferation of neighbouring wild type cells.

(Shravage et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

shrb^G5 has abnormal cytokinesis | dominant phenotype, enhanceable by l(2)gd1[+]/l(2)gd1^d7

(Matias et al., 2015)

Suppressed by

Statement

Reference

shrb^G5 has abnormal cytokinesis | dominant phenotype, suppressible by aurB^35.33/aurB[+]

(Matias et al., 2015)

shrb^G5 has abnormal cytokinesis | dominant phenotype, suppressible by aurB^2A43/aurB[+]

(Matias et al., 2015)

Phenotype Manifest In

Enhanced by

Statement

Reference

shrb^G5 has female germline stem cell phenotype, enhanceable by l(2)gd1[+]/l(2)gd1^d7

(Matias et al., 2015)

Suppressed by

Statement

Reference

shrb^G5 has female germline stem cell phenotype, suppressible by Scer\GAL4^{VP16.nanos.UTR}/Usp8^HMS01941

(Mathieu et al., 2022)

shrb^G5 has female germline cell phenotype, suppressible by aurB^2A43/aurB[+]

(Matias et al., 2015)

shrb^G5 has female germline stem cell phenotype, suppressible by aurB^2A43/aurB[+]

(Matias et al., 2015)

shrb^G5 has egg chamber phenotype, suppressible by l(2)gd1[+]/l(2)gd1^d7

(Matias et al., 2015)

shrb^G5 has female germline cell phenotype, suppressible by l(2)gd1[+]/l(2)gd1^d7

(Matias et al., 2015)

shrb^G5 has egg chamber phenotype, suppressible by aurB^35.33/aurB[+]

(Matias et al., 2015)

shrb^G5 has female germline cell phenotype, suppressible by aurB^35.33/aurB[+]

(Matias et al., 2015)

shrb^G5 has female germline stem cell phenotype, suppressible by aurB^35.33/aurB[+]

(Matias et al., 2015)

shrb^G5 has egg chamber phenotype, suppressible by aurB^2A43/aurB[+]

(Matias et al., 2015)

shrb^G5 has eye disc | somatic clone | cell non-autonomous phenotype, suppressible by Atg6¹

(Shravage et al., 2013)

Enhancer of

Statement

Reference

shrb^G5/shrb[+] is an enhancer of eye photoreceptor cell phenotype of Hsap\HTT^GMR.Q120

(Rusten et al., 2007)

Suppressor of

Statement

Reference

shrb^G5 is a suppressor | partially of egg chamber phenotype of Scer\GAL4^{VP16.nanos.UTR}, Usp8^HMS01941

(Mathieu et al., 2022)

shrb^G5 is a suppressor | partially of nucleus | oogenesis | decreased number phenotype of Scer\GAL4^{VP16.nanos.UTR}, Usp8^HMS01941

(Mathieu et al., 2022)

shrb^G5 is a suppressor of wing vein L5 phenotype of Df(3L)ru-22/rho^ve-1

(Tjota et al., 2011)

shrb^G5 is a suppressor of wing vein L5 phenotype of rho^ve-1

(Tjota et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

The fraction of egg chambers containing 32 cells is reduced in l(2)gd1^d7/shrb^G5 double heterozygotes compared to shrb^G5 heterozygotes. The fraction of germaria containing 'stem cysts' in which the female germline stem cells are interconnected to daughter cells is increased in l(2)gd1^d7/shrb^G5 double heterozygotes compared to shrb^G5 heterozygotes.

The fraction of egg chambers containing 32 cells and the fraction of germaria containing 'stem cysts' in which the female germline stem cells are interconnected to daughter cells is reduced in aurB^2A43/shrb^G5 double heterozygotes compared to shrb^G5 heterozygotes.

The fraction of egg chambers containing 32 cells and the fraction of germaria containing 'stem cysts' in which the female germline stem cells are interconnected to daughter cells is reduced in aurB^35.33/shrb^G5 double heterozygotes compared to shrb^G5 heterozygotes.

(Matias et al., 2015)

Atg6¹ suppresses the non-autonomous overgrowth seen in shrb^G5 mutant eye disc clones.

(Shravage et al., 2013)

A shrb^G5 heterozygous background leads to almost full suppression of the L5 wing vein defect in rho^ve-1/Df(3L)ru-22 kst¹ mutants and 80% suppression in rho^ve-1/rho^ve-1 kst¹ flies.