FlyBase Allele Report: Dmel\Arf6[1]

General Information

Symbol

Dmel\Arf6¹

Species

D. melanogaster

Name

FlyBase ID

FBal0230504

Feature type

allele

Associated gene

Dmel\Arf6

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Dyer et al., 2007)

Progenitor genotype

P{EP}Arf6^EP2612

(Dyer et al., 2007)

Cytology

Description

Imprecise excision of the progenitor insertion, resulting in a 1709bp deletion in the Arf51F transcription unit, which completely removes the open reading frame.

(Dyer et al., 2007)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cytokinesis | male

(Dyer et al., 2007)

female semi-sterile

(Dyer et al., 2007)

male sterile

(Dyer et al., 2007)

viable

(Dyer et al., 2007)

Phenotype Manifest In

cleavage furrow & primary spermatocyte

(Dyer et al., 2007)

mechanosensory medial triple row bristle | decreased number

(Marcetteau et al., 2021)

primary spermatocyte

(Dyer et al., 2007)

spermatid

(Dyer et al., 2007)

wing margin bristle | decreased number

(Marcetteau et al., 2021)

wing margin bristle | decreased number (with Arf6^GX16w-)

(Marcetteau et al., 2021)

Detailed Description

Statement

Reference

Arf51F¹ hetero- and homozygotes exhibit loss of wing margin bristles; heterozygotes show loss of stout bristles.

(Marcetteau et al., 2021)

Homozygous progeny from homozygous mothers survive to adulthood, with no overt external morphological defects.

Mutant females show reduced fertility.

Mutant males are completely sterile. Mutant spermatids have a "four-wheel-drive" phenotype, indicating a cytokinesis defect during spermatocyte meiosis; more than 90% of spermatids have more than one nucleus, and 41% have four nuclei per Nebenkern derivative. 1.9% of cells have 8:1 nucleus:Nebenkern ratios.

The spindle initially forms normally in mutant spermatocytes.

Cytokinesis fails in 89% of mutant spermatocytes. Cytokinesis fails early during furrow progression in 55% of the failing cells ("early regressors"). In the "early regressors", anaphase B-cell elongation occurs. The equator contracts at a rate only slightly slower than in wild type. However, fast equator contraction does not occur, only accelerating slightly, in contrast to wild type, and indentation occurs 10 minutes after central spindle pav protein accumulation (instead of 5 minutes as occurs in wild type). Shortly after the cleavage furrow indentation appears, when it is around 15μm wide, the furrow regresses. The remaining 45% of cells are "late regressors", in which cytokinesis proceeds with normal kinetics of furrow formation and progression until the furrow is less than 10μm wide. The furrow stays at this diameter for a variable time period before collapsing. During furrow progression, plasma membrane is also added with normal kinetics.

(Dyer et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

Arf6¹ has wing margin bristle | decreased number phenotype, enhanceable by wg[+]/wg^l-17

(Marcetteau et al., 2021)