FB2024_03 , released June 25, 2024
Allele: Dmel\spenKK100153
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General Information
Symbol
Dmel\spenKK100153
Species
D. melanogaster
Name
FlyBase ID
FBal0230599
Feature type
allele
Associated gene
Dmel\spen
Associated Insertion(s)
Carried in Construct
P{KK100153}
Key Links
Genomic Maps

Transgenic product class
RNAi_reagent
(Keleman et al., 2009.8.5)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Keleman et al., 2009.8.5)
Mutagen
in vitro construct
(Keleman et al., 2009.8.5)
Progenitor genotype
Carried in construct
P{KK100153}
Cytology
Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
dsRNA-KK100153
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      exacerbates  brain cancer
      modeled by Ras85DV12.cUa.UAS, scrib673
      (Manzanero-Ortiz et al., 2021)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Adulthood-generated midgut clones expressing spenKK100153 under the control of Scer\GAL4Tub.PU have more cells and show a severe increase in the number of Intestinal stem cells (Dl-positive), as compared to control clones.

      Adulthood-induced expression of spenKK100153 (using Gal80[ts] for the temporal control of expression) under the control of either Scer\GAL4esg.PU, Scer\GAL4Su(H).GBE, Scer\GAL4pros-V1, Scer\GAL4NP0001, or Scer\GAL4esg.PU plus Scer\GAL80Su(H).GBE, leads to a significant increase in the number of mitotic cells per posterior midgut, as compared to control; adulthood-induced under the control of Scer\GAL4esg.PU, Scer\GAL4Su(H).GBE, or Scer\GAL4esg.PU plus Scer\GAL80Su(H).GBE, but not under the control of Scer\GAL4pros-V1 or Scer\GAL4NP0001, also leads to a significant increase in the density of stem cells in the posterior midgut, as compared to controls.

      (Andriatsilavo et al., 2018)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      spenKK100153
      Name Synonyms
      Secondary FlyBase IDs
        References (4)