FB2024_03 , released June 25, 2024
Allele: Dmel\hpoKK101704
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General Information
Symbol
Dmel\hpoKK101704
Species
D. melanogaster
Name
FlyBase ID
FBal0231432
Feature type
allele
Associated gene
Dmel\hpo
Associated Insertion(s)
Carried in Construct
P{KK101704}
Also Known As
UAS-hpo RNAi, UAS-hpo-RNAi, UAS-hpoRNAi, hpo RNAi, hpo-RNAi, hpo-IR
Key Links
Genomic Maps

Transgenic product class
RNAi_reagent
(Keleman et al., 2009.8.5)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Keleman et al., 2009.8.5)
Mutagen
in vitro construct
(Keleman et al., 2009.8.5)
Progenitor genotype
Carried in construct
P{KK101704}
Cytology
Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
dsRNA-KK101704
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  carcinoma
      CEA
      (Kong et al., 2022)
      model of  cancer
      CEA
      (Guo et al., 2020)
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      model of  carcinoma
      is ameliorated by Toll-6ACT.UAS.Flag
      (Kong et al., 2022)
      model of  cancer
      is ameliorated by Rox8EY02351
      (Guo et al., 2020)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class

      abnormal neuroanatomy | somatic clone | third instar larval stage, with Scer\GAL4Tub.PU, Scer\GAL80ts.αTub84B

      (Poon et al., 2016)

      abnormal neuroanatomy | third instar larval stage, with Scer\GAL4insc-Mz1407

      (Poon et al., 2016)

      abnormal planar polarity | adult stage, with Scer\GAL4ey.PH

      (Brittle et al., 2012)

      abnormal size | adult stage, with Scer\GAL4nub.PU

      (Hevia et al., 2017)

      abnormal size | somatic clone | third instar larval stage, with Scer\GAL4Tub.PU, Scer\GAL80ts.αTub84B

      (Poon et al., 2016)

      abnormal size | third instar larval stage, with Scer\GAL4hh-Gal4

      (Mach et al., 2018)

      abnormal size | third instar larval stage, with Scer\GAL4insc-Mz1407

      (Poon et al., 2016)

      abnormal size | third instar larval stage, with Scer\GAL4ptc-559.1

      (Mach et al., 2018)

      decreased size | pupal stage, with Scer\GAL4Mef2.PR

      (Kaya-Çopur et al., 2021)

      flightless, with Scer\GAL4Mef2.PR

      (Kaya-Çopur et al., 2021)

      increased cell number | third instar larval stage, with Scer\GAL4repo.PU

      (Reddy and Irvine, 2011)

      increased occurrence of cell division | adult stage, with Scer\GAL4NP5130

      (Nie et al., 2015)

      increased size | adult stage, with Scer\GAL4GMR.PU

      (Guo et al., 2020)

      increased size | adult stage, with Scer\GAL4ptc.PU

      (Guo et al., 2020)

      melanotic mass phenotype | adult stage, with Scer\GAL4pnr-MD237

      (Mok and Choi, 2022)

      viable, with Scer\GAL4Mef2.PR

      (Kaya-Çopur et al., 2021)

      visible | adult stage, with Scer\GAL4GMR.PU

      (Guo et al., 2020)

      visible | adult stage, with Scer\GAL4pnr-MD237

      (Mok and Choi, 2022)

      visible | adult stage, with Scer\GAL4ptc.PU

      (Guo et al., 2020)
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The expression of hpoKK101704 under the control of Scer\GAL4Mef2.PR leads to indirect flight muscle atrophy during pupal development after 32h APF.

      (Kaya-Çopur et al., 2021)

      Expressing hpoKK101704 under the control of Scer\GAL4ptc.PU results in a reduced area between L3 and L4 in the wing.

      Expression under the control of Scer\GAL4GMR.PU leads to an enlarged eye.

      (Guo et al., 2020)

      Expressing hpoKK101704 under the control of Scer\GAL4ptc-559.1 results in a significant expansion of the anterior-posterior compartment boundary of the wing disc, while expression under the control of Scer\GAL4hh-Gal4 causes an expansion of the wing disc posterior compartment in third instar larvae.

      (Mach et al., 2018)

      The expression of hpoKK101704 under the control of Scer\GAL4nub.PU (together with Dicer-2, for efficient RNAi) leads to a significant increase in wing size, as compared to controls.

      (Hevia et al., 2017)

      Expressing hpoKK101704 under the control of Scer\GAL4insc-Mz1407 (in combination with a Dicer-2 transgene to enhance RNAi efficiency) results in a significant volume increase of the central nervous system at third instar larval stage.

      Somatic clones of type I lineages expressing hpoKK101704 under the control of Scer\GAL4Tub.PU show a significant increase in volume in the ventral nerve cord compared to controls.

      Type I neuroblasts in somatic clones expressing hpoKK101704 under the control of Scer\GAL4NP1316 (in combination with a Dicer-2 transgene to enhance RNAi efficiency) maintain cell polarity typical of wild-type controls.

      (Poon et al., 2016)

      Expression of hpoKK101704 under the control of Scer\GAL4esg-NP5130 (in combination with a Gal80[ts] transgene to restrict expression to the adult stage) results in a significant increase in the number of mitotic cells in the midgut, as compared to controls.

      (Nie et al., 2015)

      Expression of hpoKK101704 (co-expressed with Dcr-2Scer\UAS.cDa) under the control of Scer\GAL4repo.PU substantially increases glial cell numbers in the third instar larval eye disc and brain.

      (Reddy and Irvine, 2011)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Suppressed by
      Suppressor of
      Other
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The double co-expression of hpoKK101704 and SmoxNIG.2262R under the control of Scer\GAL4nub.PU (together with Dicer-2, for efficient RNAi) suppresses the wing defects induced by the single expression conditions.

      (Hevia et al., 2017)

      Co-expression of bunGD1392 suppresses the increased number of mitotic cells seen in the midguts of flies expressing hpoKK101704 under the control of Scer\GAL4esg-NP5130.

      (Nie et al., 2015)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      hpoKK101704
      (Forest et al., 2018)
      Name Synonyms
      Secondary FlyBase IDs
        References (27)