FlyBase Allele Report: Dmel\spri[KK105547]

General Information

Symbol

Dmel\spri^KK105547

Species

D. melanogaster

Name

FlyBase ID

FBal0233633

Feature type

allele

Associated gene

Dmel\spri

Associated Insertion(s)

Carried in Construct

P{KK105547}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK105547}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK105547

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell size | adult stage | somatic clone, with Scer\GAL4^Act5C.PU

(Bourouis et al., 2019)

sterile, with Scer\GAL4^ppl.PU

(Bourouis et al., 2019)

Phenotype Manifest In

autolysosome | larval stage | somatic clone, with Scer\GAL4^Act5C.PU

(Bourouis et al., 2019)

autophagosome | larval stage | somatic clone, with Scer\GAL4^Act5C.PU

(Bourouis et al., 2019)

early endosome | larval stage | somatic clone, with Scer\GAL4^Act5C.PU

(Bourouis et al., 2019)

embryonic/larval fat body | larval stage | somatic clone, with Scer\GAL4^Act5C.PU

(Bourouis et al., 2019)

lysosome | larval stage | somatic clone, with Scer\GAL4^Act5C.PU

(Bourouis et al., 2019)

Detailed Description

Statement

Reference

In 3h-starved larvae, fat body clones expressing spri^KK105547 under the control of Scer\GAL4^Act5C.PU show smaller autophagosomes ( GFP:Atg8 ) and small-sized or tiny lysosomes (LysoTracker) as compared to controls. A fraction of cells show aborted fusions into autolysosomes and some show impaired endosomal trafficking as revealed by early endosomes (i.e. Hrs marker accumulation). The clonal cells show a small but significant increase in size when generated in fed animals,

(Bourouis et al., 2019)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

NOT Suppressor of

Statement

Reference

spri^KK105547, Scer\GAL4^ninaE.PU is a non-suppressor of lipid droplet | adult stage phenotype of ND-42^ninaE.GD6220, Scer\GAL4^ninaE.PU

(Moulton et al., 2021)