FlyBase Allele Report: Dmel\drk[KK109233]

General Information

Symbol

Dmel\drk^KK109233

Species

D. melanogaster

Name

FlyBase ID

FBal0236156

Feature type

allele

Associated gene

Dmel\drk

Associated Insertion(s)

Carried in Construct

P{KK109233}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK109233}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK109233

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

glial cell, with Scer\GAL4^drl.442

(Lu et al., 2014)

glial cell, with Scer\GAL4^repo, Scer\GAL80^ts.αTub84B

(Lu et al., 2014)

vesicle, with Scer\GAL4^drl.442

(Lu et al., 2014)

vesicle, with Scer\GAL4^repo, Scer\GAL80^ts.αTub84B

(Lu et al., 2014)

Detailed Description

Statement

Reference

Glial expression of drk^KK109233, under the control of Scer\GAL4^repo (and Scer\GAL80^ts.αTub84B) suppresses glial clearance of axonal debris after axotomy. In control animals, the vast majority of axonal debris is cleared approximately 5 days after axotomy. In these mutants, a significant amount of axonal debris lingers in the brain at day 5 and is ultimately cleared 20 days after axotomy.

Knockdown of drk in glia, through expression of drk^KK109233 under the control of Scer\GAL4^repo (and Scer\GAL80^ts.αTub84B) results in a failure of the glia to efficiently form enclosed vesicles 1 day after axotomy, resulting in the total number of vesicles being reduced to ~30% that of controls.

Expression of drk^KK109233 in TIFR-positive glia (under the control of Scer\GAL4^TIFR signficiantly reduces the number of glial vesicles containing axonal debris compared with controls.

(Lu et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Suppressor of

Statement

Reference

drk^KK109233/Scer\GAL4^GMR.PU is a non-suppressor of visible phenotype of hid^Ala5.GMR

(Schoenherr et al., 2012)

drk^KK109233/Scer\GAL4^GMR.PU is a non-suppressor of visible phenotype of hid^GMR.PG

(Schoenherr et al., 2012)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^repo, Scer\GAL80^ts.αTub84B, drk^KK109233 has vesicle phenotype, suppressible by Sos^JC2/Sos[+]

(Lu et al., 2014)

Scer\GAL4^drl.442, drk^KK109233 has glial cell phenotype, suppressible by Sos^JC2/Sos[+]

(Lu et al., 2014)

NOT Suppressor of

Statement

Reference

drk^KK109233/Scer\GAL4^GMR.PU is a non-suppressor of eye phenotype of hid^Ala5.GMR

(Schoenherr et al., 2012)

drk^KK109233/Scer\GAL4^GMR.PU is a non-suppressor of eye phenotype of hid^GMR.PG

(Schoenherr et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

A Sos^JC2/+ background is sufficient to suppress the loss of glial membrane vesicles in drk knockdown flies. In addition, the internalization of axonal debris into these vesicles rises to wild-type levels in a Sos^JC2/+ background.

(Lu et al., 2014)

Co-expression of drk^KK109233 does not modify eye size in animals expressing W^GMR.PG or W^Ala5.GMR.

Co-expression of drk^KK109233 in a W^Ala5.GMR, Ack^Scer\UAS.cSb, Scer\GAL4^GMR.PU background results in flies with eyes that are greatly reduced in size.

(Schoenherr et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v105498

P{KK109233}VIE-260B

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

drk^KK109233

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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