Expression of Dh44^KK110160 under the control of Scer\GAL4^nSyb.PU in females results in a large, significant decrease in the latency to sperm and mating plug ejection, from >1 hour in controls to within 10 mins after the end of copulation (AEC); a significant reduction in the number of eggs laid in the 48h period AEC, and a significant reduction in the number of sperm in the seminal receptacle 3 h after mating..

Expression of Dh44^KK110160 under the control of Scer\GAL4^nSyb.PU in females does not result in any change in copulation duration or the number of sperm initially transferred to the uterus immediately post-copulation, and eggs produced from these females are fertile and develop to adulthood normally.

Expression of Dh44^KK110160 under the control of Scer\GAL4^Dh44.PL in females results in a significant decrease in the latency to sperm ejection, and this phenotype is maintained when GAL4 activity is restricted with the inclusion of Scer\GAL80^ChAT.3.3P.

Expression of Dh44^KK110160 under the control of Scer\GAL4^Sik2.PL in females results in a significant decrease in the latency to sperm ejection.

Expression of Dh44^KK110160 under the control of Scer\GAL4^elav-C155 (in the presence of Dcr-2^Scer\UAS.cDa to increase RNAi efficiency) results in a degradation of circadian locomotor activity rhythms under constant darkness conditions compared to controls.