FB2024_03 , released June 25, 2024
Allele: Dmel\snS52A.UASp.EGFP
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General Information
Symbol
Dmel\snS52A.UASp.EGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0239092
Feature type
allele
Associated gene
Dmel\sn
Associated Insertion(s)
Carried in Construct
P{UASp-sn.S52A.EGFP}
Key Links
Transgenic product class
missense_variant
(Zanet et al., 2009)
polypeptide_post_translational_processing_variant
(Zanet et al., 2009)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Zanet et al., 2009)
polypeptide_post_translational_processing_variant
(Zanet et al., 2009)
Mutagen
in vitro construct
(Zanet et al., 2009)
Progenitor genotype
Carried in construct
P{UASp-sn.S52A.EGFP}
Cytology
Description

Amino acid replacement: S52A.

(Zanet et al., 2009)

UASp regulatory sequences drive expression of a full-length sn cDNA (RH62992), in which codon 52 has been mutagenised to encode an unphosphorylatable A instead of a S, with EGFP fused to the N-terminus.

(Zanet et al., 2009)
Allele components
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
sn
Tagged with
EGFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      snS52A.UASp.EGFP/Scer\GAL4srp.Hemo rescues Df(1)C128

      (Zanet et al., 2009)

      Scer\GAL4VP16.nanos.UTR/snS52A.UASp.EGFP rescues sn28

      (Zanet et al., 2009)

      snS52A.UASp.EGFP/Scer\GAL4hs.PB rescues sn3

      (Zanet et al., 2009)
      Partially rescues

      snS52A.UASp.EGFP/Scer\GAL4srp.Hemo partially rescues snX2

      (Zanet et al., 2009)
      Comments

      Scer\GAL4srp.Hemo-driven expression of snS52A.Scer\UAS.P\T.T:Avic\GFP-EGFP restores the polarity and the dynamic leading edge cortex that is normally lacking in plasmatocytes in Df(1)C128 embryos. Normal developmental migration of plasmatocytes is also restored.

      Scer\GAL4srp.Hemo-driven expression of snS52A.Scer\UAS.P\T.T:Avic\GFP-EGFP provides significant rescue of the reduced plasmatocyte recruitment seen in snX2 embryos after laser-induced wounding.

      Scer\GAL4nos.UTR.T:Hsim\VP16-driven expression of snS52A.Scer\UAS.P\T.T:Avic\GFP-EGFP fully restores the formation of actin bundles around nurse cell nuclei that is normally lacking in sn28 mutants. Female ferility is also restored.

      Scer\GAL4hs.PB-driven expression of snS52A.Scer\UAS.P\T.T:Avic\GFP-EGFP fully rescues the gnarled bristle phenotype exhibited by sn3 mutants.

      (Zanet et al., 2009)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      snS52A.Scer\UAS.P\T.T:Avic\GFP-EGFP
      snS52A.UASp.EGFP
      Name Synonyms
      Secondary FlyBase IDs
        References (2)