UAS regulatory sequences drive expression of an inverted repeat.
Expression of Not1JF03096 under the control of Scer\GAL4P2.4.Pdf does not affect the oscillation of per and tim proteins in Pdf-positive pacemaker neurons, but it dampens the oscillation of Pdf protein in the axons (under the standard light:dark conditions) as well as the amplitude of free-running (in constant dark conditions) circadian locomotor activity rhythm.
Expression of Not1JF03096 (together with UAS-Dicer2 to enhance RNAi efficiency) under the control of Scer\GAL4bam.T:Hsim\VP16 results in highly penetrant tumorigenic phenotype due to spermatogonial overgrowth in adult testes.
Not1JF03096, Scer\GAL4P2.4.Pdf has abnormal locomotor rhythm | adult stage phenotype, enhanceable by Atx2[+]/Atx206490
Not1JF03096, Scer\GAL4GMR.PF is an enhancer of visible | adult stage phenotype of Hsap\MAPTUAS.cWa, Scer\GAL4GMR.PF
Not1JF03096, Scer\GAL4GMR.PF is an enhancer of eye phenotype of Hsap\MAPTUAS.cWa, Scer\GAL4GMR.PF
The poor rhythmicity of adult circadian locomotor activity under free-running (~constant darkness) conditions observed in flies expressing Not1JF03096 under the control of Scer\GAL4P2.4.Pdf is further worsened by combination with a single copy of Atx206490.