FlyBase Allele Report: Dmel\FASN1[HM05141]

General Information

Symbol

Dmel\FASN1^HM05141

Species

D. melanogaster

Name

FlyBase ID

FBal0240270

Feature type

allele

Associated gene

Dmel\FASN1

Associated Insertion(s)

Carried in Construct

P{TRiP.HM05141}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Perkins et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Perkins et al., 2009)

Mutagen

in vitro construct

(Perkins et al., 2009)

Progenitor genotype

Carried in construct

P{TRiP.HM05141}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat.

(Perkins et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HM05141

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

male fertile, with Scer\GAL4^bam.PU

(François et al., 2023)

visible, with Scer\GAL4^Bx-MS1096

(Friedman et al., 2011)

Phenotype Manifest In

embryonic/larval fat body, with Scer\GAL4^ppl.PP

(Xu et al., 2021)

embryonic/larval fat body | third instar larval stage, with Scer\GAL4^DcG

(Ugrankar et al., 2019)

fat cell | third instar larval stage, with Scer\GAL4^DcG

(Ugrankar et al., 2019)

lipid droplet | larval stage, with Scer\GAL4^ppl.PP

(Xu et al., 2021)

lipid droplet | third instar larval stage, with Scer\GAL4^DcG

(Ugrankar et al., 2019)

mitochondrial matrix | larval stage, with Scer\GAL4^ppl.PP

(Xu et al., 2021)

mitochondrion | larval stage, with Scer\GAL4^ppl.PP

(Xu et al., 2021)

wing, with Scer\GAL4^Bx-MS1096

(Friedman et al., 2011)

Detailed Description

Statement

Reference

Expressing FASN1^HM05141 under the control of Scer\GAL4^DcG leads to viable third instar larvae that contain a normal amount of fat tissue but appear to lack fat stores, as extracted fat bodies sink to the bottom of PBS-containing reservoirs, unlike floating lipid-filled controls. Fat cells exhibit a decrease in the size and an increase in the density of peripheral lipid droplets although these remain intact, adjacent to the cell surface, compared to controls; there are no changes in cytoplasmic medial lipid droplet size or density.

(Ugrankar et al., 2019)

Expression of CG3523^HM05141 under the control of Scer\GAL4^Bx-MS1096 results in slight curling of the wing.

(Friedman et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

NOT Enhanced by

Statement

Reference