FlyBase Allele Report: Dmel\ASPP[8]

General Information

Symbol

Dmel\ASPP⁸

Species

D. melanogaster

Name

FlyBase ID

FBal0240346

Feature type

allele

Associated gene

Dmel\ASPP

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Langton et al., 2007)

Mutagen

P-element activity

(Langton et al., 2007)

Progenitor genotype

P{XP}d03020

(Langton et al., 2007)

Caused by aberration

Df(2R)ASPP⁸

Cytology

Description

Imprecise excision of the progenitor insertion, resulting in a deletion of approximately 29kb that removes the CG15561 transcription unit and all of the ASPP coding sequence.

(Langton et al., 2007)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | adult stage (with ASPP^d)

(Bertran et al., 2019)

increased cell number

(Langton et al., 2007)

increased cell number | adult stage (with ASPP^d)

(Bertran et al., 2019)

increased cell number | pupal stage (with ASPP^d)

(Bertran et al., 2019)

visible

(Langton et al., 2007)

visible | adult stage (with ASPP^d)

(Bertran et al., 2019)

Phenotype Manifest In

eye

(Langton et al., 2007)

interommatidial bristle

(Langton et al., 2007)

interommatidial bristle | pupal stage (with ASPP^d)

(Bertran et al., 2019)

interommatidial cell | pupal stage (with ASPP^d)

(Bertran et al., 2019)

ommatidium

(Langton et al., 2007)

scutellar bristle | increased number (with ASPP^d)

(Bertran et al., 2019)

wing

(Langton et al., 2007)

wing (with ASPP^d)

(Bertran et al., 2019)

Detailed Description

Statement

Reference

ASPP^d/ASPP⁸ transheterozygotes exhibit significantly more inter-ommatidial cells and frequently misplaced bristles in the pupal eye, as compared to controls; adults show larger wings and frequent duplication of scutellar bristles.

(Bertran et al., 2019)

Mutant adults are 6.1% heavier than controls.

Homozygous flies have an enlarged, rough eye phenotype compared to controls. There is disorganisation of the ommatidial lattice, with missing or extra interommatidial bristle cells. Clustering of the bristle cells is often seen and the bristle cells frequently contact four or more cells (wild-type bristle cells contact three neighbours). The mutant ommatidia often have five vertices, instead of six (as occurs in wild type).

There is no increase in the number of cells per ommatidium in the pupal retina of ASPP⁸ animals, but adult eyes have significantly more ommatidia (796 +/- 24 per eye) compared to controls (747 +/- 25 per eye).

Mutant adults have enlarged wings (on average 11.8% larger than normal). The density of wing hairs in the mutant wing is comparable to that of controls, indicating that the increase in wing size is due to an increase in the number of cells rather than an increase in cell size.

Homozygous clones do not have any apparent growth advantage over control clones.

(Langton et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

ASPP^d/ASPP⁸, Csk^j1D8 has visible | adult stage phenotype, suppressible | partially by ASPP^{FA.Ubi-p63E.GFP}

(Bertran et al., 2019)

ASPP^d/ASPP⁸, Csk^j1D8 has visible | adult stage phenotype, suppressible by ASPP^Ubi-p63E.GFP

(Bertran et al., 2019)

ASPP⁸, Csk^j1D8/Csk[+] has visible phenotype, suppressible by Btk29A[+]/Btk^k00206

(Langton et al., 2007)

ASPP⁸ has visible phenotype, suppressible by Btk29A[+]/Btk^k00206

(Langton et al., 2007)

ASPP⁸ has visible phenotype, suppressible by Src64B^ko/Src64B^ko

(Langton et al., 2007)

Enhancer of

Statement

Reference

ASPP^d, ASPP^{FA.Ubi-p63E.GFP}, ASPP⁸ is an enhancer of increased cell number | pupal stage phenotype of Df(2L)Exel7014/Ccdc85^C1.1

(Bertran et al., 2019)

ASPP^{KVK.Ubi-p63E.GFP}, ASPP^d, ASPP⁸ is an enhancer of increased cell number | pupal stage phenotype of Df(2L)Exel7014/Ccdc85^C1.1

(Bertran et al., 2019)

ASPP^{WK.Ubi-p63E.GFP}, ASPP^d, ASPP⁸ is an enhancer of increased cell number | pupal stage phenotype of Df(2L)Exel7014/Ccdc85^C1.1

(Bertran et al., 2019)

ASPP^d, ASPP^{FA-WK.Ubi-p63E.GFP}, ASPP⁸ is an enhancer of increased cell number | pupal stage phenotype of Df(2L)Exel7014/Ccdc85^C1.1

(Bertran et al., 2019)

ASPP^d/ASPP⁸ is an enhancer of increased cell number | pupal stage phenotype of Df(2L)Exel7014/Ccdc85^C1.1

(Bertran et al., 2019)

ASPP[+]/ASPP⁸ is an enhancer of visible phenotype of Csk^j1D8, puc^E69/puc[+]

(Langton et al., 2007)

NOT Enhancer of

Statement

Reference

ASPP^Ubi-p63E.GFP, ASPP^d, ASPP⁸ is a non-enhancer of increased cell number | pupal stage phenotype of Df(2L)Exel7014/Ccdc85^C1.1

(Bertran et al., 2019)

ASPP[+]/ASPP⁸ is a non-enhancer of visible | adult stage phenotype of Magi^UAS.GFP, Scer\GAL4^GMR.PU

(Zaessinger et al., 2015)

NOT Suppressor of

Statement

Reference

ASPP[+]/ASPP⁸ is a non-suppressor of visible | adult stage phenotype of Magi^UAS.GFP, Scer\GAL4^GMR.PU

(Zaessinger et al., 2015)

Other

Statement

Reference

ASPP^d/ASPP⁸, Csk^j1D8 has visible | adult stage phenotype

(Bertran et al., 2019)

ASPP[+]/ASPP⁸, Csk^j1D8, puc^E69/puc[+] has visible phenotype

(Langton et al., 2007)

ASPP⁸, Csk^j1D8/Csk[+] has visible phenotype

(Langton et al., 2007)

ASPP⁸, Csk^j1D8/Csk[+] has increased cell death | third instar larval stage phenotype

(Langton et al., 2007)

ASPP⁸, puc^E69/puc[+] has increased cell death | third instar larval stage phenotype

(Langton et al., 2007)

Phenotype Manifest In

Suppressed by

Statement

Reference

ASPP^d/ASPP⁸, Csk^j1D8 has wing phenotype, suppressible by ASPP^Ubi-p63E.GFP

(Bertran et al., 2019)

ASPP^d/ASPP⁸, Csk^j1D8 has wing phenotype, suppressible | partially by ASPP^{FA.Ubi-p63E.GFP}

(Bertran et al., 2019)

ASPP⁸, Csk^j1D8/Csk[+] has wing phenotype, suppressible by Btk29A[+]/Btk^k00206

(Langton et al., 2007)

ASPP⁸, Csk^j1D8/Csk[+] has wing disc phenotype, suppressible by Btk29A[+]/Btk^k00206

(Langton et al., 2007)

ASPP⁸ has eye phenotype, suppressible by Btk29A[+]/Btk^k00206

(Langton et al., 2007)

ASPP⁸ has eye phenotype, suppressible by Src64B^ko/Src64B^ko

(Langton et al., 2007)

Enhancer of

Statement

Reference

ASPP^d, ASPP^{FA.Ubi-p63E.GFP}, ASPP⁸ is an enhancer of interommatidial cell | pupal stage phenotype of Df(2L)Exel7014/Ccdc85^C1.1

(Bertran et al., 2019)

ASPP^d, ASPP^{FA.Ubi-p63E.GFP}, ASPP⁸ is an enhancer of interommatidial bristle | pupal stage phenotype of Df(2L)Exel7014/Ccdc85^C1.1

(Bertran et al., 2019)

ASPP^{KVK.Ubi-p63E.GFP}, ASPP^d, ASPP⁸ is an enhancer of interommatidial cell | pupal stage phenotype of Df(2L)Exel7014/Ccdc85^C1.1

(Bertran et al., 2019)

ASPP^{KVK.Ubi-p63E.GFP}, ASPP^d, ASPP⁸ is an enhancer of interommatidial bristle | pupal stage phenotype of Df(2L)Exel7014/Ccdc85^C1.1

(Bertran et al., 2019)

ASPP^{WK.Ubi-p63E.GFP}, ASPP^d, ASPP⁸ is an enhancer of interommatidial cell | pupal stage phenotype of Df(2L)Exel7014/Ccdc85^C1.1

(Bertran et al., 2019)

ASPP^{WK.Ubi-p63E.GFP}, ASPP^d, ASPP⁸ is an enhancer of interommatidial bristle | pupal stage phenotype of Df(2L)Exel7014/Ccdc85^C1.1

(Bertran et al., 2019)

ASPP^d, ASPP^{FA-WK.Ubi-p63E.GFP}, ASPP⁸ is an enhancer of interommatidial cell | pupal stage phenotype of Df(2L)Exel7014/Ccdc85^C1.1

(Bertran et al., 2019)

ASPP^d, ASPP^{FA-WK.Ubi-p63E.GFP}, ASPP⁸ is an enhancer of interommatidial bristle | pupal stage phenotype of Df(2L)Exel7014/Ccdc85^C1.1

(Bertran et al., 2019)

ASPP^d/ASPP⁸ is an enhancer of eye | pupal stage phenotype of Df(2L)Exel7014/Ccdc85^C1.1

(Bertran et al., 2019)

ASPP^d/ASPP⁸ is an enhancer of interommatidial bristle | pupal stage phenotype of Df(2L)Exel7014/Ccdc85^C1.1

(Bertran et al., 2019)

ASPP[+]/ASPP⁸ is an enhancer of wing phenotype of Csk^j1D8, puc^E69/puc[+]

(Langton et al., 2007)

ASPP⁸/ASPP⁸ is an enhancer of wing disc phenotype of Csk^RNAi.UAS, Scer\GAL4^ptc-559.1

(Langton et al., 2007)

NOT Enhancer of

Statement

Reference

ASPP^Ubi-p63E.GFP, ASPP^d, ASPP⁸ is a non-enhancer of interommatidial cell | pupal stage phenotype of Df(2L)Exel7014/Ccdc85^C1.1

(Bertran et al., 2019)

ASPP^Ubi-p63E.GFP, ASPP^d, ASPP⁸ is a non-enhancer of interommatidial bristle | pupal stage phenotype of Df(2L)Exel7014/Ccdc85^C1.1

(Bertran et al., 2019)

ASPP[+]/ASPP⁸ is a non-enhancer of eye | adult stage phenotype of Magi^UAS.GFP, Scer\GAL4^GMR.PU

(Zaessinger et al., 2015)

NOT Suppressor of

Statement

Reference

ASPP[+]/ASPP⁸ is a non-suppressor of eye | adult stage phenotype of Magi^UAS.GFP, Scer\GAL4^GMR.PU

(Zaessinger et al., 2015)

Other

Statement

Reference

ASPP^d/ASPP⁸, Csk^j1D8 has wing phenotype

(Bertran et al., 2019)

ASPP[+]/ASPP⁸, Csk^j1D8, puc^E69/puc[+] has leg phenotype

(Langton et al., 2007)

ASPP⁸, Csk^j1D8/Csk[+] has wing phenotype

(Langton et al., 2007)

ASPP⁸, Csk^j1D8/Csk[+] has wing disc phenotype

(Langton et al., 2007)

ASPP⁸, puc^E69/puc[+] has wing disc phenotype

(Langton et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

ASPP^d/ASPP⁸, Csk^j1D8/+ adults very frequently show wing notches, which are never observed in controls; this phenotype is fully rescued by ASPP^Ubi-p63E.GFP, but only partially by ASPP^{FA.Ubi-p63E.GFP}.

(Bertran et al., 2019)

The rough eye phenotype observed in adults expressing Magi^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^GMR.PU is not modified by combination with single copy of ASPP⁸.

(Zaessinger et al., 2015)

ASPP⁸/ASPP⁸ Csk^j1D8/+ adults show a notched wing phenotype. Wing discs of ASPP⁸/ASPP⁸ Csk^j1D8/+ third instar larvae show considerable apoptosis. Neither phenotype is seen in ASPP⁸/ASPP⁸ or Csk^j1D8/+ single mutants.

Btk29A^k00206/+ largely suppresses the ectopic apoptosis in wing discs and notched wing phenotype seen in ASPP⁸/ASPP⁸ Csk^j1D8/+ animals.

The eye phenotype of ASPP⁸ homozygotes is suppressed by Btk29A^k00206/+ and by Src64B^ko/Src64B^ko.

The ectopic apoptosis seen in the wing discs of puc^E69/+ Csk^j1D8/+ double heterozygotes is enhanced if they are also heterozygous for ASPP⁸, resulting in adults with a severely reduced wing size. These adults also have leg deformities.

The wing discs of ASPP⁸/ASPP⁸ puc^E69/+ third instar larvae show a moderate ectopic patch of apoptotic cells. Adults of this genotype do not show wing notching.

ASPP⁸/ASPP⁸ dramatically enhances the delamination, movement and apoptosis phenotype seen in Csk mutant cells which are at the boundary between mutant and wild-type cells in wing discs expressing Csk^IR.Scer\UAS under the control of Scer\GAL4^ptc-559.1.

(Langton et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

ASPP^d/ASPP⁸ is rescued by ASPP^Ubi-p63E.GFP

(Bertran et al., 2019)

ASPP⁸ is rescued by Scer\GAL4^da.G32/ASPP^UASp.cLa

(Langton et al., 2007)

Partially rescued by

ASPP^d/ASPP⁸ is partially rescued by ASPP^{FA.Ubi-p63E.GFP}

(Bertran et al., 2019)

ASPP^d/ASPP⁸ is partially rescued by ASPP^{KVK.Ubi-p63E.GFP}

(Bertran et al., 2019)

ASPP^d/ASPP⁸ is partially rescued by ASPP^{FA-WK.Ubi-p63E.GFP}

(Bertran et al., 2019)

Comments

ASPP^d/ASPP⁸ transheterozygotes show increased numbers of inter-ommatidial cells and misplaced bristles in the pupal eye, as well as the enlarged wings in adults, all of which are fully rescued by ASPP^Ubi-p63E.GFP but only partially rescued by ASPP^{FA.Ubi-p63E.GFP}, ASPP^{KVK.Ubi-p63E.GFP}, ASPP^{FA-WK.Ubi-p63E.GFP}, or ASPP^{WK.Ubi-p63E.GFP}. ASPP^d/ASPP⁸ transheterozygotes also show duplicated scutellar bristles, which are fully and partially suppressed in males and females, respectively, by ASPP^Ubi-p63E.GFP, and partially suppressed in both males and females by ASPP^{FA.Ubi-p63E.GFP}.

(Bertran et al., 2019)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

ASPP⁸

(Chan et al., 2021, Bertran et al., 2019, Zaessinger et al., 2015)

dASPP⁸

(Langton et al., 2007)

Name Synonyms

Secondary FlyBase IDs

References (4)

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