FlyBase Allele Report: Dmel\mgr[G5308]

General Information

Symbol

Dmel\mgr^G5308

Species

D. melanogaster

Name

FlyBase ID

FBal0241589

Feature type

allele

Associated gene

Dmel\mgr

Associated Insertion(s)

P{EP}mgr^G5308

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Bellen, 2010.2.16)

Progenitor genotype

Associated Insertion(s)

P{EP}mgr^G5308

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:11,409,825..11,409,825 [-]

(Bellen, 2010.2.16)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | third instar larval stage

(Zhang et al., 2016)

abnormal neuroanatomy | third instar larval stage (with Df(3R)Exel6160)

(Zhang et al., 2016)

increased cell number | third instar larval stage

(Zhang et al., 2016)

increased cell number | third instar larval stage (with Df(3R)Exel6160)

(Zhang et al., 2016)

Phenotype Manifest In

centrosome | third instar larval stage (with Df(3R)Exel6160)

(Zhang et al., 2016)

mitotic spindle | third instar larval stage (with Df(3R)Exel6160)

(Zhang et al., 2016)

neuroblast | increased number | third instar larval stage (with Df(3R)Exel6160)

(Zhang et al., 2016)

spindle microtubule | third instar larval stage

(Zhang et al., 2016)

Detailed Description

Statement

Reference

mgr^G5308/Df(3R)Exel6160 mutants show ectopic neuroblasts in third instar larval brains and defects in asymmetric protein segregation in neuroblasts (both during metaphase and telophase) as well as mitotic spindle orientation. mgr^G5308/Df(3R)Exel6160 neuroblasts often contain irregular number of centrosomes during mitosis (either more than two or just one - leading to monopolar mitotic spindle) and majority of them fail to assemble astral microtubules during metaphase.

(Zhang et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

mgr^G5308 has increased cell number | third instar larval stage phenotype, enhanceable by pins^P89

(Zhang et al., 2016)

mgr^G5308 has abnormal neuroanatomy phenotype, enhanceable by Pfdn2^Δ10

(Zhang et al., 2016)

mgr^G5308 has abnormal neuroanatomy | third instar larval stage phenotype, enhanceable by pins^P89

(Zhang et al., 2016)

Enhancer of

Statement

Reference

mgr^G5308 is an enhancer of abnormal neuroanatomy | third instar larval stage phenotype of Pfdn2^Δ10

(Zhang et al., 2016)

Suppressor of

Statement

Reference

mgr^G5308 is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of pins^P89

(Zhang et al., 2016)

mgr^G5308 is a suppressor of decreased cell number | third instar larval stage phenotype of pins^P89

(Zhang et al., 2016)

Phenotype Manifest In

Enhanced by

Statement

Reference

mgr^G5308 has neuroblast | third instar larval stage phenotype, enhanceable by Pfdn2^Δ10

(Zhang et al., 2016)

mgr^G5308 has mitotic spindle | third instar larval stage phenotype, enhanceable by Pfdn2^Δ10

(Zhang et al., 2016)

mgr^G5308 has centrosome | third instar larval stage phenotype, enhanceable by Pfdn2^Δ10

(Zhang et al., 2016)

Enhancer of

Statement

Reference

mgr^G5308 is an enhancer of neuroblast | third instar larval stage phenotype of Pfdn2^Δ10

(Zhang et al., 2016)

mgr^G5308 is an enhancer of mitotic spindle | third instar larval stage phenotype of Pfdn2^Δ10

(Zhang et al., 2016)

mgr^G5308 is an enhancer of centrosome | third instar larval stage phenotype of Pfdn2^Δ10

(Zhang et al., 2016)

mgr^G5308 is an enhancer of spindle microtubule | third instar larval stage phenotype of Pfdn2^Δ10

(Zhang et al., 2016)

Suppressor of

Statement

Reference

mgr^G5308 is a suppressor of neuroblast | third instar larval stage phenotype of pins^P89

(Zhang et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

The increased average number of brain neuroblasts (compared to wild-type) seen in mgr^G5308 homozygous third instar larvae is strongly enhanced by combination with pins^P89 although pins^P89 mutants on their own display decreased number of brain neuroblasts compared to wild-type.

The microtubule and centrosomal abnormalities (irregular number of centrosomes and spindle defects, including loss of astral microtubules) observed in brain neuroblasts in Pfdn2^Δ10 single mutant and mgr^G5308/Df(3R)Exel6160 third instar larvae are exacerbated further in Pfdn2^Δ10,mgr^G5308 double mutants.

(Zhang et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

30151

w¹¹¹⁸; P{EP}mgr^G5308/TM6C, Sb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

CG6719^G5308

(Bellen, 2010.2.16)

mgr^G5308

(Zhang et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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