A notable decrease in the size of salivary gland nuclei is observed in ~21% of cells when cdc23dsRNA.WIZ.Scer\UAS is expressed in 'flip out' clones in salivary glands.
Sporadic occurrence of melanotic tissue is seen in third instar larvae expressing cdc23dsRNA.WIZ.Scer\UAS under the control of Scer\GAL4Act5C.
Animals expressing cdc23dsRNA.WIZ.Scer\UAS under the control of Scer\GAL4Act5C die in the P4(ii) (moving bubble) stage of prepupal development (some lethality is also seen during the third instar larval stage).
Brain neuroblasts from third instar larvae expressing cdc23dsRNA.WIZ.Scer\UAS under the control of Scer\GAL4Act5C show metaphase-like arrest with overcondensed chromosomes. the chromosomes in most of the arrested cells appear scattered, while in about 10% of mitotic cells the chromosomes are locked at the metaphase plate. Some cells appear polyploid. Lagging chromosomes and chromosome bridges are detected in anaphase figures. The mitotic index is almost double that of wild type. The metaphase:anaphase ratio is also increased compared to wild type. The larval brains show higher levels of apoptosis than controls.
Cdc23RNAi.WIZ.UAS, Scer\GAL4Act5C.PP has abnormal endomitotic cell cycle | somatic clone phenotype, enhanceable by APC10RNAi.UAS, Scer\GAL4Act5C.PP
APC10RNAi.UAS, Cdc23RNAi.WIZ.UAS, Scer\GAL4Act5C.PP has abnormal endomitotic cell cycle | somatic clone phenotype, non-suppressible by gemininRNAi.UAS, Scer\GAL4Act5C.PP
Cdc23RNAi.WIZ.UAS, Scer\GAL4Act5C.PP has nucleus | somatic clone phenotype, enhanceable by APC10RNAi.UAS, Scer\GAL4Act5C.PP
APC10RNAi.UAS, Cdc23RNAi.WIZ.UAS, Scer\GAL4Act5C.PP has nucleus | somatic clone phenotype, non-suppressible by gemininRNAi.UAS, Scer\GAL4Act5C.PP
Co-expression of cdc23dsRNA.WIZ.Scer\UAS and Apc10dsRNA.Scer\UAS in 'flip out' clones in salivary glands results in a dramatic decrease in nuclear size in 43% of cells.
Co-expression of geminindsRNA.Scer\UAS with cdc23dsRNA.WIZ.Scer\UAS and Apc10dsRNA.Scer\UAS in 'flip out' clones in salivary glands does not rescue the endoreplication defects associated with expression of cdc23dsRNA.WIZ.Scer\UAS and Apc10dsRNA.Scer\UAS.
Animals co-expressing cdc16dsRNA.WIZ.Scer\UAS and cdc23dsRNA.WIZ.Scer\UAS under the control of Scer\GAL4Act5C show greatly increased larval lethality and occurrence of melanotic tumours compared to animals expressing either cdc16dsRNA.WIZ.Scer\UAS or cdc23dsRNA.WIZ.Scer\UAS singly under the control of Scer\GAL4Act5C. There is also a shift in pupal lethality towards earlier stages in the double mutant animals.
Animals co-expressing Apc7dsRNA.WIZ.Scer\UAS and cdc23dsRNA.WIZ.Scer\UAS under the control of Scer\GAL4Act5C show greatly increased larval lethality and occurrence of melanotic tumours compared to animals expressing cdc23dsRNA.WIZ.Scer\UAS singly under the control of Scer\GAL4Act5C. There is also a shift in pupal lethality towards earlier stages in the double mutant animals.
The mitotic index of brain neuroblasts in larvae co-expressing Apc7dsRNA.WIZ.Scer\UAS and cdc23dsRNA.WIZ.Scer\UAS under the control of Scer\GAL4Act5C is significantly higher than that in larvae expressing cdc23dsRNA.WIZ.Scer\UAS singly under the control of Scer\GAL4Act5C.
The mitotic index of brain neuroblasts in larvae co-expressing cdc16dsRNA.WIZ.Scer\UAS and cdc23dsRNA.WIZ.Scer\UAS under the control of Scer\GAL4Act5C is significantly higher than that in larvae expressing either cdc16dsRNA.WIZ.Scer\UAS or cdc23dsRNA.WIZ.Scer\UAS singly under the control of Scer\GAL4Act5C.