Imprecise excision of the P{EP}EP1526 insertion, resulting in a deletion that is predicted to produce a truncated pcm protein of 1293 amino acid residues.
Intragenic deletion which removes 516 bp resulting in a frame-shift. Protein is C-terminally truncated by 348 aa compared to wildtype, with a 29 aa extension encoded from out-of-frame pcm sequences. The 3' UTR is intact.
Estimated boundaries of a 516 bp deletion resulting from the imprecise excision of P{EP}EP1526, which removes parts of exons 8 and 9 of pcm. The protein is missing the C-terminal residues starting at aa1264.
P-body | cold sensitive (with Df(1)JA27)
pcm5 mutant adults have slightly but significantly smaller wings compared to controls.
pcm5 is cold sensitive.
At 19[o]C 98% of pcm5 adults have dull wings, crumpled along the posterior edge with occasional blisters in the posterior half of the wing. This is reduced to 68% at 25[o]C. The surface of the wing is not smooth, but has a regular pattern of raised tissue under each trichome.
Approximately half of pcm5 mutant flies have crooked legs, where the third pair of legs is bent within the femur. This phenotype is not observed in newly eclosed flies, but arises in the adult over time due to a weakness in the legs. This phenotype is most penetrant at lower temperatures.
The denticle belt patterning is not perturbed in pcm5 mutant embryos.
Wounded pcm5 homozygous flies have reduced survival rates compared to control flies at both 19[o]C and 25[o]C. The clotting process in mutants follows a similar time course to that of controls.
Male and female flies homozygous (or hemizygous) for pcm5 show no dorsal bald patches at both 19[o]C and 25[o]C.
pcm5/Df(1)JA27 animals have much reduced zygotic viability at 19[o]C compared to heterozygous siblings, but there is little effect on viability at 25[o]C.
pcm5/Df(1)JA27 adult females have a dull-wing phenotype at all temperatures.
At 19[o]C pcm5/Df(1)JA27 pupae show a severe cleft thorax defect and die before emergence. Homozygous pcm5 or hemizygous pcm5/Y mutants show a lower frequency of cleft thorax defects.
80% of pcm5/Df(1)JA27 embryos show a dorsal-open phenotype at 19[o]C. At 25[o]C these embryos show a severe dorsal closure defect.
pcm5 homozygous mutant females lay fewer eggs than controls and many egg chambers degenerate around stage 9.
The average size and number of P bodies (detected using P{YFP-Dcp1.L}) in nurse cell cytoplasm in ovaries from 18[o]C reared pcm5/Df(1)JA27 females is increased to ~180% and ~145% compared to that of controls, respectively.
The average size and number of P bodies (detected using Dcp2 antibody) in nurse cell cytoplasm in ovaries from 18[o]C reared pcm5/Df(1)JA27 females is increased to ~200% compared to that of controls.
pcm5 has abnormal size | adult stage phenotype, suppressible by Scer\GAL469B/Dis3l2GD9240
pcm5 has wing phenotype, non-enhanceable by pucA251.1F3/puc[+]
pcm5 has wing phenotype, suppressible by Scer\GAL469B/Dis3l2GD9240
pcm5/pcm5 is a suppressor of sex comb tooth | ectopic phenotype of Psc1
pcm5, pucA251.1F3/puc[+] has adult thorax phenotype
The ectopic sex comb teeth phenotype of Psc1 heterozygous males is partially suppressed by homozygosity for pcm5; this phenotype suppression by pcm5 homozygosity is annulled either by heterozygosity for mbf12 or by the heat-shock-induced expression of pcmhs.PG. The ectopic sex comb teeth phenotype of Psc1 heterozygous males is exacerbated by the triple combinations of heterozygosity for mbf12, homozygosity for either pcmΔ1 or pcm5 and heat-shock-induced expression of pcmhs.PG.
The reduced wing size characteristic for pcm5 mutant adults is fully rescued by expression of Dis3l2GD9240 under the control of Scer\GAL469B in the mutant background.
Male and female flies homozygous (or hemizygous) for pcm5 and heterozygous for pucA251.1F3 are viable, but over half show a bald patch at the anterior end of the dorsal midline at 19[o]C, indicating that thorax closure is not quite complete. At 25[o]C the majority of flies have bald patches.
Male and female flies homozygous (or hemizygous) for pcm5 and heterozygous for pucA251.1F3 have dull wings at 19[o]C and 25[o]C.
