FlyBase Allele Report: Dmel\if[JF02695]

General Information

Symbol

Dmel\if^JF02695

Species

D. melanogaster

Name

FlyBase ID

FBal0242283

Feature type

allele

Associated gene

Dmel\if

Associated Insertion(s)

Carried in Construct

P{TRiP.JF02695}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Perkins et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Perkins et al., 2009)

Mutagen

in vitro construct

(Perkins et al., 2009)

Progenitor genotype

Carried in construct

P{TRiP.JF02695}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat.

(Perkins et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-JF02695

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of intellectual disability

CEA

(Haddadi and Ataei, 2022)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates cancer

modeled by Hsap\CDH3^UASp.cPa

(Faria et al., 2023)

ameliorates Huntington's disease

modeled by Hsap\HTT^{128Q.1-231.UAS}

(Hernandez et al., 2023)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic/larval optic stalk | early third instar larval stage, with Scer\GAL4^repo

(Tavares et al., 2015)

embryonic/larval optic stalk | second instar larval stage, with Scer\GAL4^repo

(Tavares et al., 2015)

embryonic/larval optic stalk | third instar larval stage, with Scer\GAL4^repo

(Tavares et al., 2015)

extracellular matrix | third instar larval stage, with Scer\GAL4^repo

(Tavares et al., 2015)

eye disc | early third instar larval stage, with Scer\GAL4^repo

(Tavares et al., 2015)

eye disc | second instar larval stage, with Scer\GAL4^repo

(Tavares et al., 2015)

eye disc | third instar larval stage, with Scer\GAL4^repo

(Tavares et al., 2015)

eye photoreceptor cell | third instar larval stage, with Scer\GAL4^repo

(Tavares et al., 2015)

glial cell | early third instar larval stage, with Scer\GAL4^repo

(Tavares et al., 2015)

glial cell | second instar larval stage, with Scer\GAL4^repo

(Tavares et al., 2015)

glial cell | third instar larval stage, with Scer\GAL4^repo

(Tavares et al., 2015)

visceral muscle fiber | third instar larval stage | heat sensitive, with Scer\GAL4^Mef2.PR, Scer\GAL80^ts.αTub84B

(Liu et al., 2013)

Detailed Description

Statement

Reference

Expressing if^JF02695 under the control of Scer\GAL4^repo (in combination with a Dicer-2 transgene to enhance RNAi efficiency) results in decreased glial cell numbers and an abnormal distribution of glial cells in the eye discs and optic stalks of second and third instar larvae. Together with a lack of cell death, this points towards defective cell migration. Photoreceptor axon attachment to the neural lamella and targeting to the optic stalk are unaffected.

Third instar larvae expressing if^JF02695 under the control of Scer\GAL4^repo exhibit unwrapped, defasciculated and disorganized photoreceptor axon bundles, and a severely reduced fenestrated membrane in eye discs. The neural lamella is unaffected.

(Tavares et al., 2015)

Scer\GAL4^Mef2.PR-mediated expression of if^JF02695, induced at the end of the first larval instar stage using the Scer\GAL80 system, results in muscle attachment defects.

(Liu et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^repo, if^JF02695 has decreased cell number | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^repo/btl::Egfr^λ.UAS

(Tavares et al., 2015)

NOT Enhancer of

Statement

Reference

if^JF02695/Scer\GAL4^repo.PU is a non-enhancer of increased size | larval stage phenotype of Scer\GAL4^repo.PU, kon^{UAS.Tag:HA,Tag:SS(wg)}

(Harrison et al., 2021)

if^JF02695/Scer\GAL4^elav.PU is a non-enhancer of increased size | larval stage phenotype of Scer\GAL4^elav.PU, kon^{UAS.Tag:HA,Tag:SS(wg)}

(Harrison et al., 2021)

Suppressor of

Statement

Reference

if^JF02695, Scer\GAL4^repo is a suppressor of decreased rate of adult locomotory behavior phenotype of Hsap\HTT^{128Q.1-231.UAS}, Scer\GAL4^repo

(Hernandez et al., 2023)

if^JF02695, Scer\GAL4^elav-C155 is a suppressor | partially of decreased rate of adult locomotory behavior | progressive phenotype of Hsap\HTT^{128Q.1-231.UAS}, Scer\GAL4^elav-C155

(Hernandez et al., 2023)

if^JF02695/Scer\GAL4^repo is a suppressor | partially of decreased cell number | third instar larval stage phenotype of Scer\GAL4^repo, btl::Egfr^λ.UAS

(Tavares et al., 2015)

NOT Suppressor of

Statement

Reference

if^JF02695/Scer\GAL4^repo.PU is a non-suppressor of increased size | larval stage phenotype of Scer\GAL4^repo.PU, kon^{UAS.Tag:HA,Tag:SS(wg)}

(Harrison et al., 2021)

if^JF02695/Scer\GAL4^elav.PU is a non-suppressor of increased size | larval stage phenotype of Scer\GAL4^elav.PU, kon^{UAS.Tag:HA,Tag:SS(wg)}

(Harrison et al., 2021)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^repo, if^JF02695 has eye disc | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^repo/btl::Egfr^λ.UAS

(Tavares et al., 2015)

Scer\GAL4^repo, if^JF02695 has embryonic/larval optic stalk | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^repo/btl::Egfr^λ.UAS

(Tavares et al., 2015)

Scer\GAL4^repo, if^JF02695 has glial cell | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^repo/btl::Egfr^λ.UAS

(Tavares et al., 2015)

NOT Enhancer of

Statement

Reference

if^JF02695/Scer\GAL4^repo.PU is a non-enhancer of larval ventral nerve cord phenotype of Scer\GAL4^repo.PU, kon^{UAS.Tag:HA,Tag:SS(wg)}

(Harrison et al., 2021)

if^JF02695/Scer\GAL4^elav.PU is a non-enhancer of larval ventral nerve cord phenotype of Scer\GAL4^elav.PU, kon^{UAS.Tag:HA,Tag:SS(wg)}

(Harrison et al., 2021)

Suppressor of

Statement

Reference

if^JF02695/Scer\GAL4^repo is a suppressor | partially of glial cell | third instar larval stage phenotype of Scer\GAL4^repo, btl::Egfr^λ.UAS

(Tavares et al., 2015)

if^JF02695/Scer\GAL4^repo is a suppressor | partially of eye disc | third instar larval stage phenotype of Scer\GAL4^repo, btl::Egfr^λ.UAS

(Tavares et al., 2015)

if^JF02695/Scer\GAL4^repo is a suppressor | partially of embryonic/larval optic stalk | third instar larval stage phenotype of Scer\GAL4^repo, btl::Egfr^λ.UAS

(Tavares et al., 2015)

NOT Suppressor of

Statement

Reference

if^JF02695/Scer\GAL4^repo.PU is a non-suppressor of larval ventral nerve cord phenotype of Scer\GAL4^repo.PU, kon^{UAS.Tag:HA,Tag:SS(wg)}

(Harrison et al., 2021)

if^JF02695/Scer\GAL4^elav.PU is a non-suppressor of larval ventral nerve cord phenotype of Scer\GAL4^elav.PU, kon^{UAS.Tag:HA,Tag:SS(wg)}

(Harrison et al., 2021)

Additional Comments

Genetic Interactions

Statement

Reference

Larvae co-expressing btl::Egfr^λ.UAS alongside if^JF02695 under the control of Scer\GAL4^repo show milder defects in eye disc glial cell numbers than either single expression condition.

(Tavares et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

27544

y¹ v¹; P{TRiP.JF02695}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Int α PS2^RNAi

(Kim et al., 2018)

if^JF02695

if^RNAi

(Haddadi and Ataei, 2022)

αPS2^RNAi

(Zhai et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (11)

Report Sections

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