FlyBase Allele Report: Dmel\Mical[15256]

General Information

Symbol

Dmel\Mical¹⁵²⁵⁶

Species

D. melanogaster

Name

FlyBase ID

FBal0242680

Feature type

allele

Associated gene

Dmel\Mical

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Kirilly et al., 2009)

Progenitor genotype

Cytology

Description

Amino acid replacement: Q535term.

(Kirilly et al., 2009)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:10,019,780..10,019,780 [-]

Nucleotide change:

C10019780T

Amino acid change:

Reported amino acid change:

Q535term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Beuchle et al., 2007)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy

(Kirilly et al., 2009)

abnormal neuroanatomy (with Df(3R)swp2^MICAL)

(Kirilly et al., 2009)

abnormal neuroanatomy | pupal stage | somatic clone

(Wong et al., 2013)

Phenotype Manifest In

dendrite

(Kirilly et al., 2009)

dendrite (with Df(3R)swp2^MICAL)

(Kirilly et al., 2009)

larval dorsal multidendritic neuron ddaC

(Kirilly et al., 2009)

larval dorsal multidendritic neuron ddaC (with Df(3R)swp2^MICAL)

(Kirilly et al., 2009)

larval dorsal multidendritic neuron ddaC | pupal stage | somatic clone

(Wong et al., 2013)

Detailed Description

Statement

Reference

Mical¹⁵²⁵⁶ mutant ddaC neuron clones exhibit dendrite pruning defects at 16 hours after puparium formation. No difference is seen at the white prepupal stage.

(Wong et al., 2013)

Mical¹⁵²⁵⁶ and Mical¹⁵²⁵⁶/Df(3R)swp2^MICAL mutants exhibit a failure of dendrite severing in ddaC neurons by 18h APF. Mical¹⁵²⁵⁶ ddaC neurons retain an average of 7.4 primary and secondary dendrites attached to the soma at 18h APF and 3 dendrites at 24h APF, indicating that the mutant neurons have a strong severing defect. The overall larval dendrite morphology of ddaC neurons in the wL3 larvae of Mical¹⁵²⁵⁶ zygotic mutants is indistinguishable from that of wild-type ddaC neurons, as judged by the number of terminal dendrites.

Mical¹⁵²⁵⁶ ddaC MARCM clones show a severing defect in ddaC neurons.

(Kirilly et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Mical¹⁵²⁵⁶ has abnormal neuroanatomy | pupal stage | somatic clone phenotype, enhanceable by slmb⁸

(Wong et al., 2013)

Mical¹⁵²⁵⁶ has abnormal neuroanatomy | pupal stage | somatic clone phenotype, enhanceable by InR^A1325D.UAS/Scer\GAL4^ppk.PG

(Wong et al., 2013)

Mical¹⁵²⁵⁶ has abnormal neuroanatomy | pupal stage | somatic clone phenotype, enhanceable by Pi3K92E^UAS.Exel/Scer\GAL4^ppk.PG

(Wong et al., 2013)

Mical¹⁵²⁵⁶ has abnormal neuroanatomy | pupal stage | somatic clone phenotype, enhanceable by Rheb^UAS.cPa/Scer\GAL4^ppk.PG

(Wong et al., 2013)

Suppressed by

Statement

Reference

Mical¹⁵²⁵⁶ has abnormal neuroanatomy phenotype, suppressible | partially by Sox14^fl.UAS/Scer\GAL4^ppk.1.9

(Kirilly et al., 2009)

Mical¹⁵²⁵⁶ has abnormal neuroanatomy phenotype, suppressible | partially by Sox14^fl.UAS/Scer\GAL4²²¹

(Kirilly et al., 2009)

Enhancer of

Statement

Reference

Mical¹⁵²⁵⁶/Mical[+] is an enhancer of abnormal neuroanatomy | pupal stage P5 phenotype of Df(3R)Exel7310/prd1^M56

(Zong et al., 2018)

Mical¹⁵²⁵⁶/Mical[+] is an enhancer of abnormal size | pupal stage P5 phenotype of Df(3R)Exel7310/prd1^M56

(Zong et al., 2018)

Mical¹⁵²⁵⁶ is an enhancer of abnormal neuroanatomy | pupal stage | somatic clone phenotype of slmb⁸

(Wong et al., 2013)

Suppressor of

Statement

Reference

Mical¹⁵²⁵⁶ is a suppressor of abnormal neuroanatomy phenotype of Scer\GAL4^ppk.1.9, Sox14^fl.UAS

(Kirilly et al., 2009)

Mical¹⁵²⁵⁶ is a suppressor of abnormal neuroanatomy phenotype of Scer\GAL4²²¹, Sox14^fl.UAS

(Kirilly et al., 2009)

Phenotype Manifest In

Enhanced by

Statement

Reference

Mical¹⁵²⁵⁶ has larval dorsal multidendritic neuron ddaC | pupal stage | somatic clone phenotype, enhanceable by slmb⁸

(Wong et al., 2013)

Mical¹⁵²⁵⁶ has larval dorsal multidendritic neuron ddaC | pupal stage | somatic clone phenotype, enhanceable by InR^A1325D.UAS/Scer\GAL4^ppk.PG

(Wong et al., 2013)

Mical¹⁵²⁵⁶ has larval dorsal multidendritic neuron ddaC | pupal stage | somatic clone phenotype, enhanceable by Pi3K92E^UAS.Exel/Scer\GAL4^ppk.PG

(Wong et al., 2013)

Mical¹⁵²⁵⁶ has larval dorsal multidendritic neuron ddaC | pupal stage | somatic clone phenotype, enhanceable by Rheb^UAS.cPa/Scer\GAL4^ppk.PG

(Wong et al., 2013)

Suppressed by

Statement

Reference

Mical¹⁵²⁵⁶ has dendrite phenotype, suppressible | partially by Sox14^fl.UAS/Scer\GAL4^ppk.1.9

(Kirilly et al., 2009)

Mical¹⁵²⁵⁶ has larval dorsal multidendritic neuron ddaC phenotype, suppressible | partially by Sox14^fl.UAS/Scer\GAL4^ppk.1.9

(Kirilly et al., 2009)

Mical¹⁵²⁵⁶ has dendrite phenotype, suppressible | partially by Sox14^fl.UAS/Scer\GAL4²²¹

(Kirilly et al., 2009)

Mical¹⁵²⁵⁶ has larval dorsal multidendritic neuron ddaC phenotype, suppressible | partially by Sox14^fl.UAS/Scer\GAL4²²¹

(Kirilly et al., 2009)

Enhancer of

Statement

Reference

Mical¹⁵²⁵⁶/Mical[+] is an enhancer of dendritic tree | pupal stage P5 phenotype of Df(3R)Exel7310/prd1^M56

(Zong et al., 2018)

Mical¹⁵²⁵⁶/Mical[+] is an enhancer of larval dorsal multidendritic neuron ddaC | pupal stage P5 phenotype of Df(3R)Exel7310/prd1^M56

(Zong et al., 2018)

Mical¹⁵²⁵⁶ is an enhancer of larval dorsal multidendritic neuron ddaC | pupal stage | somatic clone phenotype of slmb⁸

(Wong et al., 2013)

Suppressor of

Statement

Reference

Mical¹⁵²⁵⁶ is a suppressor of dendrite phenotype of Scer\GAL4²²¹, Sox14^fl.UAS

(Kirilly et al., 2009)

Mical¹⁵²⁵⁶ is a suppressor of larval dorsal multidendritic neuron ddaC phenotype of Scer\GAL4²²¹, Sox14^fl.UAS

(Kirilly et al., 2009)

Mical¹⁵²⁵⁶ is a suppressor of dendrite phenotype of Scer\GAL4^ppk.1.9, Sox14^fl.UAS

(Kirilly et al., 2009)

Mical¹⁵²⁵⁶ is a suppressor of larval dorsal multidendritic neuron ddaC phenotype of Scer\GAL4^ppk.1.9, Sox14^fl.UAS

(Kirilly et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

Mical¹⁵²⁵⁶ heterozygosity does not significantly affect the ddaC neuron pruning defects observed in prd1^M56/Df(3R)Exel7310 transheterozygous at 16h after puparium formation, namely the length of the persisting dendrites.

(Zong et al., 2018)

Mical¹⁵²⁵⁶ slmb⁸ double mutant ddaC neuron clones have more severe dendrite pruning defects at 16 hours after puparium formation as are seen in either single mutant alone. No change is seen in the level of pruning at the white prepupal stage.

Expression of InR^{A1325D.Scer\UAS} under the control of Scer\GAL4^ppk.PG enhances the dendrite pruning defects seen in Mical¹⁵²⁵⁶ mutant ddaC neuron clones at 16 hours after puparium formation.

Expression of Pi3K92E^{Scer\UAS.Exel} under the control of Scer\GAL4^ppk.PG enhances the dendrite pruning defects seen in Mical¹⁵²⁵⁶ mutant ddaC neuron clones at 16 hours after puparium formation.

Expression of Rheb^Scer\UAS.cPa under the control of Scer\GAL4^ppk.PG enhances the dendrite pruning defects seen in Mical¹⁵²⁵⁶ mutant ddaC neuron clones at 16 hours after puparium formation.

(Wong et al., 2013)

Overexpression of Sox14^fl.Scer\UAS under the control of either Scer\GAL4^ppk.1.9 or Scer\GAL4²²¹ in a homozygous Mical¹⁵²⁵⁶ background has a very mild pruning effect on primary and secondary dendrites by 6 hours APF.

(Kirilly et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Mical¹⁵²⁵⁶ is rescued by Scer\GAL4^ppk.1.9/Mical^UAS.fl

(Kirilly et al., 2009)

Mical¹⁵²⁵⁶/Df(3R)swp2^MICAL is rescued by Scer\GAL4^ppk.1.9/Mical^UAS.fl

(Kirilly et al., 2009)

Not rescued by

Mical¹⁵²⁵⁶ is not rescued by Mical^N-ter.UAS/Scer\GAL4^ppk.1.9

(Kirilly et al., 2009)

Mical¹⁵²⁵⁶/Df(3R)swp2^MICAL is not rescued by Mical^N-ter.UAS/Scer\GAL4^ppk.1.9

(Kirilly et al., 2009)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Mical¹⁵²⁵⁶

l(3)15256

(Kirilly et al., 2009)

mical¹⁵²⁵⁶

(Zong et al., 2018, Wong et al., 2013)

Name Synonyms

Secondary FlyBase IDs

References (3)

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