FlyBase Allele Report: Dmel\Cln3[UAS.cTa]

General Information

Symbol

Dmel\Cln3^UAS.cTa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Tuxworth

FlyBase ID

FBal0243514

Feature type

allele

Associated gene

Dmel\Cln3

Associated Insertion(s)

Carried in Construct

P{UAS-Cln3.T}

Also Known As

UAS-Cln3

Key Links

Transgenic product class

wild_type

(Tuxworth et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Tuxworth et al., 2009)

Mutagen

in vitro construct

(Tuxworth et al., 2009)

Progenitor genotype

Carried in construct

P{UAS-Cln3.T}

Cytology

Description

UAS regulatory sequences drive expression of wild-type Cln3 coding sequences.

(Tuxworth et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Cln3

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal oxidative stress response, with Scer\GAL4^Ilp2.215-3

(Tuxworth et al., 2011)

abnormal size | adult stage, with Scer\GAL4^GMR.PU

(Llavero Hurtado et al., 2017)

increased cell death, with Scer\GAL4^en-e16E

(Tuxworth et al., 2009)

long lived, with Scer\GAL4^Ilp2.215-3

(Tuxworth et al., 2011)

visible, with Scer\GAL4^en-e16E

(Tuxworth et al., 2011)

visible, with Scer\GAL4^GMR.PF

(Tuxworth et al., 2011)

visible | adult stage, with Scer\GAL4^GMR.PU

(Llavero Hurtado et al., 2017)

Phenotype Manifest In

embryo | dorsal closure stage, with Scer\GAL4^pnr-MD237

(Tuxworth et al., 2009)

eye, with Scer\GAL4^GMR.PF

(Tuxworth et al., 2011, Tuxworth et al., 2009)

eye, with Scer\GAL4^GMR.PU

(Llavero Hurtado et al., 2017)

interommatidial bristle, with Scer\GAL4^GMR.PU

(Jones et al., 2014)

ommatidium, with Scer\GAL4^GMR.PU

(Jones et al., 2014)

wing blade, with Scer\GAL4^en-e16E

(Tuxworth et al., 2011)

wing disc, with Scer\GAL4^en-e16E

(Tuxworth et al., 2009)

wing margin, with Scer\GAL4^en-e16E

(Tuxworth et al., 2011)

wing vein, with Scer\GAL4^en-e16E

(Tuxworth et al., 2011)

Detailed Description

Statement

Reference

The expression of Cln3^UAS.cTa under the control of Scer\GAL4^GMR.PU leads to a severe decrease in eye size, as compared to controls.

(Llavero Hurtado et al., 2017)

Scer\GAL4^GMR.PU-mediated expression of cln3^Scer\UAS.cTa leads to disorganized ommatidia and loss of eye bristles.

Negative geotaxis is not altered in flies expressing cln3^Scer\UAS.cTa via Scer\GAL4^elav.PU.

(Jones et al., 2014)

Overexpression of cln3^Scer\UAS.cTa in the developing eye under the control of Scer\GAL4^GMR.PF results in roughening and glazing of the outer surface of the adult eye.

Overexpression of cln3^Scer\UAS.cTa in the posterior half of the wing under the control of Scer\GAL4^en-e16E results in thickened veins, loss of tissue, vein deltas and occasionally notching in the wing margin.

Overexpression of cln3^Scer\UAS.cTa under the control of Scer\GAL4^Ilp2.215-3 results in increased oxidative stress resistance and a small but significant extension of life span compared with wild-type.

(Tuxworth et al., 2011)

Expression of cln3^Scer\UAS.cTa in the eye, under the control of Scer\GAL4^GMR.PF produces a significant glazing of the compound eye indicative of degeneration.

Overexpression of cln3^Scer\UAS.cTa with Scer\GAL4^da.G32 does not affect microchaetae on the thorax and there is no neurogenic phenotype.

Overexpression of cln3^Scer\UAS.cTa with Scer\GAL4^pnr-MD237 specifically in the dorsal midline leads to dorsal closure phenotypes in both the thorax and the abdomen of adult flies.

Overexpression of cln3^Scer\UAS.cTa with Scer\GAL4^en-e16E induces apoptosis in the developing wing disc.

(Tuxworth et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Cln3^UAS.cTa, Scer\GAL4^GMR.PU has abnormal size | adult stage phenotype, enhanceable by Mtpα^GD11299, Scer\GAL4^GMR.PU

(Llavero Hurtado et al., 2017)

Cln3^UAS.cTa, Scer\GAL4^GMR.PU has abnormal size | adult stage phenotype, enhanceable by Hibch^GD11513, Scer\GAL4^GMR.PU

(Llavero Hurtado et al., 2017)

Cln3^UAS.cTa, Scer\GAL4^GMR.PU has visible | adult stage phenotype, enhanceable by Hibch^GD11513, Scer\GAL4^GMR.PU

(Llavero Hurtado et al., 2017)

Cln3^UAS.cTa, Scer\GAL4^GMR.PU has visible | adult stage phenotype, enhanceable by Mtpα^GD11299, Scer\GAL4^GMR.PU

(Llavero Hurtado et al., 2017)

Cln3^UAS.cTa, Scer\GAL4^en-e16E has visible phenotype, enhanceable by bol^unspecified, Scer\GAL4^en-e16E

(Tuxworth et al., 2011)

Cln3^UAS.cTa, Scer\GAL4^GMR.PF has visible phenotype, enhanceable by bol^unspecified, Scer\GAL4^GMR.PF

(Tuxworth et al., 2011)

Cln3^UAS.cTa, Scer\GAL4^en-e16E has increased cell death phenotype, enhanceable by bsk^UAS.cNa, Scer\GAL4^en-e16E

(Tuxworth et al., 2009)

Cln3^UAS.cTa, Scer\GAL4^en-e16E has increased cell death phenotype, enhanceable by tsu[+]/tsu^Δ18/Scer\GAL4^GMR.PF

(Tuxworth et al., 2009)

Suppressed by

Statement

Reference

Cln3^UAS.cTa, Scer\GAL4^GMR.PU has abnormal size | adult stage phenotype, suppressible by Rok^JF03225, Scer\GAL4^GMR.PU

(Llavero Hurtado et al., 2017)

Cln3^UAS.cTa, Scer\GAL4^GMR.PU has visible | adult stage phenotype, suppressible by Rok^JF03225, Scer\GAL4^GMR.PU

(Llavero Hurtado et al., 2017)

Cln3^UAS.cTa, Scer\GAL4^GMR.PU has abnormal size | adult stage phenotype, suppressible by Rok^GD1522, Scer\GAL4^GMR.PU

(Llavero Hurtado et al., 2017)

Cln3^UAS.cTa, Scer\GAL4^GMR.PU has visible | adult stage phenotype, suppressible by Rok^GD1522, Scer\GAL4^GMR.PU

(Llavero Hurtado et al., 2017)

Cln3^UAS.cTa, Scer\GAL4^GMR.PF has visible phenotype, suppressible | partially by Sod1^UAS.cAa, Scer\GAL4^GMR.PF

(Tuxworth et al., 2011)

Cln3^UAS.cTa, Scer\GAL4^GMR.PF has visible phenotype, suppressible | partially by Cat^UAS.cAa, Scer\GAL4^GMR.PF

(Tuxworth et al., 2011)

Cln3^UAS.cTa, Scer\GAL4^GMR.PF has visible phenotype, suppressible | partially by foxo²¹/foxo[+]

(Tuxworth et al., 2011)

Cln3^UAS.cTa, Scer\GAL4^GMR.PF has visible phenotype, suppressible | partially by flfl^fl.UAS.Venus, Scer\GAL4^GMR.PF

(Tuxworth et al., 2011)

Cln3^UAS.cTa, Scer\GAL4^GMR.PF has visible phenotype, suppressible | partially by Keap1^EY5/Keap1[+]

(Tuxworth et al., 2011)

NOT suppressed by

Statement

Reference

Cln3^UAS.cTa, Scer\GAL4^en-e16E has increased cell death phenotype, non-suppressible by Scer\GAL4^GMR.PF/BacA\p35^UAS.cHa

(Tuxworth et al., 2009)

Enhancer of

Statement

Reference

Cln3^UAS.cTa, Scer\GAL4^GMR.PF is an enhancer of visible phenotype of Scer\GAL4^GMR.PF, foxo^UAS.cPa

(Tuxworth et al., 2011)

Phenotype Manifest In

Enhanced by

Statement

Reference

Cln3^UAS.cTa, Scer\GAL4^GMR.PU has eye phenotype, enhanceable by Mtpα^GD11299, Scer\GAL4^GMR.PU

(Llavero Hurtado et al., 2017)

Cln3^UAS.cTa, Scer\GAL4^GMR.PU has eye phenotype, enhanceable by Hibch^GD11513, Scer\GAL4^GMR.PU

(Llavero Hurtado et al., 2017)

Cln3^UAS.cTa, Scer\GAL4^GMR.PU has ommatidium phenotype, enhanceable by Cisd^G6528

(Jones et al., 2014)

Cln3^UAS.cTa, Scer\GAL4^GMR.PU has interommatidial bristle phenotype, enhanceable by Cisd^G6528

(Jones et al., 2014)

Cln3^UAS.cTa, Scer\GAL4^en-e16E has wing margin phenotype, enhanceable by bol^unspecified, Scer\GAL4^en-e16E

(Tuxworth et al., 2011)

Cln3^UAS.cTa, Scer\GAL4^en-e16E has wing vein phenotype, enhanceable by bol^unspecified, Scer\GAL4^en-e16E

(Tuxworth et al., 2011)

Cln3^UAS.cTa, Scer\GAL4^GMR.PF has eye phenotype, enhanceable by bol^unspecified, Scer\GAL4^GMR.PF

(Tuxworth et al., 2011)

Cln3^UAS.cTa, Scer\GAL4^en-e16E has wing blade phenotype, enhanceable by bol^unspecified, Scer\GAL4^en-e16E

(Tuxworth et al., 2011)

Cln3^UAS.cTa, Scer\GAL4^GMR.PF has eye phenotype, enhanceable by tsu[+]/tsu^Δ18, Scer\GAL4^GMR.PF

(Tuxworth et al., 2009)

Cln3^UAS.cTa, Scer\GAL4^en-e16E has wing disc phenotype, enhanceable by bsk^UAS.cNa, Scer\GAL4^en-e16E

(Tuxworth et al., 2009)

Suppressed by

Statement

Reference

Cln3^UAS.cTa, Scer\GAL4^GMR.PU has eye phenotype, suppressible by Rok^JF03225, Scer\GAL4^GMR.PU

(Llavero Hurtado et al., 2017)

Cln3^UAS.cTa, Scer\GAL4^GMR.PU has eye phenotype, suppressible by Rok^GD1522, Scer\GAL4^GMR.PU

(Llavero Hurtado et al., 2017)

Cln3^UAS.cTa, Scer\GAL4^GMR.PF has eye phenotype, suppressible | partially by foxo²¹/foxo[+]

(Tuxworth et al., 2011)

Cln3^UAS.cTa, Scer\GAL4^GMR.PF has eye phenotype, suppressible | partially by flfl^fl.UAS.Venus, Scer\GAL4^GMR.PF

(Tuxworth et al., 2011)

Cln3^UAS.cTa, Scer\GAL4^GMR.PF has eye phenotype, suppressible | partially by Keap1^EY5/Keap1[+]

(Tuxworth et al., 2011)

Cln3^UAS.cTa, Scer\GAL4^GMR.PF has eye phenotype, suppressible | partially by Sod1^UAS.cAa, Scer\GAL4^GMR.PF

(Tuxworth et al., 2011)

Cln3^UAS.cTa, Scer\GAL4^GMR.PF has eye phenotype, suppressible | partially by Cat^UAS.cAa, Scer\GAL4^GMR.PF

(Tuxworth et al., 2011)

NOT suppressed by

Statement

Reference

Cln3^UAS.cTa, Scer\GAL4^GMR.PF has eye phenotype, non-suppressible by BacA\p35^UAS.cHa, Scer\GAL4^GMR.PF

(Tuxworth et al., 2009)

Enhancer of

Statement

Reference

Cln3^UAS.cTa, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, foxo^UAS.cPa

(Tuxworth et al., 2011)

Suppressor of

Statement

Reference

Cln3^UAS.cTa, Scer\GAL4^GMR.PU is a suppressor | partially of ommatidium phenotype of Ppt1^UAS.cKa, Scer\GAL4^GMR.PU

(Jones et al., 2014)

Other

Statement

Reference

Cln3^UAS.cTa, Scer\GAL4^en-e16E, bsk^K53R.UAS has wing hair phenotype

(Tuxworth et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

The small eye induced by the expression of Cln3^UAS.cTa under the control of Scer\GAL4^GMR.PU is fully suppressed by the co-expression of Rok^JF03225, is partially suppressed by the co-expression of Rok^GD1522 and is enhanced by the co-expression of either CG5044^GD11513 or Mtpα^GD11299.

(Llavero Hurtado et al., 2017)

Negative geotaxis behaviour is substantially reduced in flies expressing cln3^Scer\UAS.cTa via Scer\GAL4^elav.PU in a Cisd2^G6528 background.

(Jones et al., 2014)

Co-expression of bol^unspecified under the control of Scer\GAL4^GMR.PF enhances the cln3^Scer\UAS.cTa-overexpression phenotypes in the eye.

Co-expression of bol^unspecified under the control of Scer\GAL4^en-e16E enhances the cln3^Scer\UAS.cTa-overexpression phenotypes in the wing.

Co-expression of cln3^Scer\UAS.cTa under the control of Scer\GAL4^GMR.PF enhances the foxo^Scer\UAS.cPa-overexpression phenotype in the eye by producing a much wider glazing.

The eye phenotype resulting from the overexpression of cln3^Scer\UAS.cTa under the control of Scer\GAL4^GMR.PF is partially suppressed by foxo²¹/+.

Co-expression of flfl^{fl.Scer\UAS.T:Avic\GFP-YFP.Venus} under the control of Scer\GAL4^GMR.PF almost completely suppresses the cln3^Scer\UAS.cTa-overexpression phenotype in the eye.

The eye phenotype resulting from the overexpression of cln3^Scer\UAS.cTa under the control of Scer\GAL4^GMR.PF is almost completely suppressed by Keap1^EY5/+.

Co-expression of Sod^Scer\UAS.cAa under the control of Scer\GAL4^GMR.PF almost completely suppresses the cln3^Scer\UAS.cTa-overexpression phenotype in the eye.

Co-expression of Cat^Scer\UAS.cAa under the control of Scer\GAL4^GMR.PF almost completely suppresses the cln3^Scer\UAS.cTa-overexpression phenotype in the eye.

(Tuxworth et al., 2011)

A tsu^Δ18 heterozygous background enhances the cln3^Scer\UAS.cTa overexpression eye phenotype.

Overexpression of both bsk^{K53R.Scer\UAS} and cln3^Scer\UAS.cTa under the control of Scer\GAL4^en-e16E produces clones of cells in the wings with multiple bristles.

Overexpression of both bsk^Scer\UAS.cNa and cln3^Scer\UAS.cTa in the wing disc under the control of Scer\GAL4^en-e16E leads to a dramatic increase in cln3^Scer\UAS.cTa phenotype, with almost complete loss of tissue in the posterior half suggesting extensive apoptosis.

(Tuxworth et al., 2009)

Xenogenetic Interactions

Statement

Reference

Overexpression of BacA\p35^Scer\UAS.cHa with Scer\GAL4^GMR.PF fails to suppress the cln3^Scer\UAS.cTa overexpression phenotype.

(Tuxworth et al., 2009)

Complementation and Rescue Data

Rescued by

Cln3^UAS.cTa is rescued by Scer\GAL4^GMR.PF/Cln3^GD2699

(Tuxworth et al., 2009)

Comments

Co-expression of cln3^GD2699 with cln3^Scer\UAS.cTa, both under the control of Scer\GAL4^GMR.PF rescues the eye degeneration phenotype.

(Tuxworth et al., 2009)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Cln3^Scer\UAS.cTa

Cln3^UAS.cTa

cln3^Scer\UAS.cTa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Tuxworth

Secondary FlyBase IDs

References (4)

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