FB2024_03 , released June 25, 2024
Allele: Dmel\StimUAS.cAa
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General Information
Symbol
Dmel\StimUAS.cAa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Agrawal
FlyBase ID
FBal0243769
Feature type
allele
Associated gene
Dmel\Stim
Associated Insertion(s)
Carried in Construct
P{UAS-Stim.A}
Also Known As
UASdSTIM
Key Links
Transgenic product class
cDNA
(Agrawal et al., 2010)
wild_type
(Agrawal et al., 2010)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Agrawal et al., 2010)
wild_type
(Agrawal et al., 2010)
Mutagen
in vitro construct
(Agrawal et al., 2010)
Progenitor genotype
Carried in construct
P{UAS-Stim.A}
Cytology
Description

UASt regulatory sequences drive expression of a full-length Stim cDNA.

(Agrawal et al., 2010)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Stim
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of StimScer\UAS.cAa under the control of Scer\GAL4Act5C.PI does not compromise adult eclosion rate compared to controls.

      (Pathak et al., 2017)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressor of
      Other
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of StimScer\UAS.cAa and olf186-FScer\UAS.cZa driven by Scer\GAL4Ilp2.PR completely suppresses the wing posture, flight and flight physiology defects of Itp-r83Aka1091/Itp-r83Aug3 flies.

      Simultaneous expression of StimScer\UAS.cAa and olf186-FScer\UAS.cZa driven by Scer\GAL4Ddc.PL in aminergic neurons results in pupal lethality.

      Expression of StimScer\UAS.cAa driven by either Scer\GAL4Ilp2.PR or Scer\GAL4Ddc.PL suppresses the altered wing posture of Itp-r83Aka1091/Itp-r83Aug3 flies as well as the spontaneous hyperactivity of the dorsal longitudinal muscles. The flight ability and sustained air-puff-induced rhythmic flight patterns are also restored in these flies.

      Pan-neuronal expression of StimScer\UAS.cAa and olf186-FScer\UAS.cZa driven by Scer\GAL4elav-C155 in Itp-r83Aka1091/Itp-r83Aug3 flies suppresses the abnormal wing-posture to varying extents but does not fully restore flight ability.

      Overexpressing StimScer\UAS.cAa with or without olf186-FScer\UAS.cZa driven by Scer\GAL4Ilp2.PR in Itp-r83Aka1091/Itp-r83Aug3 mutant neurons restores global intracellular Ca[2+] homeostasis.

      (Agrawal et al., 2010)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Scer\GAL4Act5C.PI/StimUAS.cAa rescues Stimko

      (Pathak et al., 2017)
      Partially rescues

      StimUAS.cAa/Scer\GAL4nSyb.PS partially rescues Stimko

      (Pathak et al., 2017)

      Scer\GAL4ple.PF/StimUAS.cAa partially rescues Stimko

      (Pathak et al., 2017)

      Scer\GAL4ple.TH-A/StimUAS.cAa partially rescues Stimko

      (Pathak et al., 2017)

      Scer\GAL4elav-C155, StimUAS.cAa, Spyo\Cas9UAS.Tag:NLS(Ude) partially rescues StimsgRNA.dual.snRNA:U6:96Ab

      (Pathak et al., 2017)
      Comments

      Scer\GAL4nSyb.PS-driven expression of StimScer\UAS.cAa does not rescue small larval body size or the delay in larval development characteristic for Stimko/Stimko but it does partially rescue their lethality - as some adults manage to eclose. Similarly, the lethality but not the developmental delay is partially rescued by Scer\GAL4ple.PF- or Scer\GAL4ple.TH-A-driven expression and the lethality is almost completely rescued when StimScer\UAS.cAa is driven by Scer\GAL4Act5C.PI.

      Co-expression of StimScer\UAS.cAa fully restores the developmental delay characteristic for flies expressing StimsgRNA.dual.snRNA:U6:96Ab together with Spyo\Cas9Scer\UAS.T:nls-Ude under the control of Scer\GAL4elav-C155 (to induce tissue-specific gene knock-out) but their increased lethality is only partially rescued.

      (Pathak et al., 2017)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      StimScer\UAS.cAa
      StimUAS.cAa
      dSTIM+
      (Deb et al., 2016)
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Agrawal
      Secondary FlyBase IDs
        References (8)