FB2024_03 , released June 25, 2024
Allele: Dmel\rprRNAi.RHG.UAS
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General Information
Symbol
Dmel\rprRNAi.RHG.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0244378
Feature type
allele
Associated gene
Dmel\rpr
Associated Insertion(s)
Carried in Construct
P{UAS-RHG.RNAi}
Also Known As
UAS-miRHG, UAS-miRGH, UAS-RHG miRNA
Key Links
Transgenic product class
RNAi_reagent
(Siegrist et al., 2010)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Siegrist et al., 2010)
Mutagen
in vitro construct
(Siegrist et al., 2010)
Progenitor genotype
Carried in construct
P{UAS-RHG.RNAi}
Cytology
Description

UASt regulatory sequences drive expression of three microRNAs, directed against rpr, hid and grim respectively.

(Siegrist et al., 2010)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
rpr
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The expression of rprmiRNA.RHG.Scer\UAS under the control of Scer\GAL4Bx-MS1096 does not lead to obvious defects in tissue architecture or in growth in third instar larval wing discs, as compared to controls.

      (Bunker et al., 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressor of
      Other
      Phenotype Manifest In
      Suppressor of
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The triple expression of grimmiRNA.RHG.UAS, hidmiRNA.RHG.UAS and rprmiRNA.RHG.UAS under the control of Scer\GAL4C10 induces significantly smaller wings, with significantly fewer cells, although slightly bigger, as compared to controls. Likewise, the legs are significantly smaller than controls (femur and tarsal segment 1, but not tibia). Wing discs show a significant decrease in apoptosis (detected by PARP:VENUS signal and TUNEL assay), as compared to controls.

      The triple expression under the control of Scer\GAL4ap-md544 leads to opaque and curly wings, which indicate apoptosis and growth inhibition during development, respectively.

      (Shinoda et al., 2019)

      The eye defect caused by WGMR.PU is effectively rescued by expression of P{UAS-RHG.miRNA} (carries WmiRNA.RHG.Scer\UAS, grimmiRNA.RHG.Scer\UAS and rprmiRNA.RHG.Scer\UAS) under the control of Scer\GAL4GMR.PF.

      (Lee et al., 2013)

      Mushroom body neuroblasts are seen in the brains of young adults simultaneously co-expressing rprmiRNA.RHG.Scer\UAS, WmiRNA.RHG.Scer\UAS and grimmiRNA.RHG.Scer\UAS (from the P{UAS-RHG.miRNA} transgene) under the control of Scer\GAL4wor.PA, although their persistence is only transient (mushroom body neuroblasts are not seen in wild-type adults).

      Neuroblasts are detected in the mushroom body of 2 week old and even in 1 month old adults simultaneously co-expressing rprmiRNA.RHG.Scer\UAS, WmiRNA.RHG.Scer\UAS and grimmiRNA.RHG.Scer\UAS (from the P{UAS-RHG.miRNA} transgene) under the control of Scer\GAL4wor.PA in a foxo25 background (mushroom body neuroblasts are not seen in wild-type adults and are not seen at these late time points in adults expressing the P{UAS-RHG.miRNA} transgene under the control of Scer\GAL4wor.PA in a wild-type background or in foxo25 single mutant adults). Some of the 2 week old mutant mushroom body neuroblasts are large in size and generate many new progeny. There is a strong correlation between mushroom body neuroblast cell size and progeny number in 2 week old mutant adults, but not in younger mutant adults. In 1 month old mutant adults, some of the progeny of these neuroblasts show normal axon projections through the mushroom body pedunculus and some mistarget, bifurcating prematurely and projecting anterior to the pedunculus.

      Neuroblasts are detected in the mushroom body of 1 month old adults simultaneously co-expressing rprmiRNA.RHG.Scer\UAS, WmiRNA.RHG.Scer\UAS and grimmiRNA.RHG.Scer\UAS (from the P{UAS-RHG.miRNA} transgene) and Atg1K38Q.Scer\UAS under the control of Scer\GAL4wor.PA (mushroom body neuroblasts are not seen in wild-type adults).

      (Siegrist et al., 2010)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      microRGH
      (Velarde et al., 2021)
      rprRNAi.RHG.UAS
      rprmiRNA.RHG.Scer\UAS
      rprmiRNA.RHG.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (26)