rprRNAi.RHG.UAS, grimRNAi.RHG.UAS, hidRNAi.RHG.UAS, Scer\GAL4esg.PU is a suppressor of hyperplasia | adult stage phenotype of Scer\GAL4esg.PU, synrUAS.ORF.GW.Tag:HA
grimRNAi.RHG.UAS, Scer\GAL4CCAP.PP is a suppressor of increased cell death | third instar larval stage phenotype of EcRB1-ΔC655.W650A.UAS, Scer\GAL4CCAP.PP
grimRNAi.RHG.UAS, Scer\GAL4CCAP.PP is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of EcRB1-ΔC655.W650A.UAS, Scer\GAL4CCAP.PP
Scer\GAL4hh.PU, rprRNAi.RHG.UAS, grimRNAi.RHG.UAS, hidRNAi.RHG.UAS is a suppressor of increased cell death | third instar larval stage phenotype of Scer\GAL4hh.PU, rlGD4697
rprRNAi.RHG.UAS, grimRNAi.RHG.UAS, hidRNAi.RHG.UAS, Scer\GAL4GMR.PF is a suppressor of visible phenotype of hidGMR.PU
Scer\GAL4nub-AC-62, grimRNAi.RHG.UAS, hepCA.UAS, hidRNAi.RHG.UAS, rprRNAi.RHG.UAS has increased size | larval stage phenotype
Scer\GAL4C10, grimRNAi.RHG.UAS, hidRNAi.RHG.UAS, rprRNAi.RHG.UAS has decreased cell death | larval stage phenotype
Scer\GAL4C10, grimRNAi.RHG.UAS, hidRNAi.RHG.UAS, rprRNAi.RHG.UAS has abnormal size | adult stage phenotype
Scer\GAL4C10, grimRNAi.RHG.UAS, hidRNAi.RHG.UAS, rprRNAi.RHG.UAS has increased cell size | adult stage phenotype
Scer\GAL4C10, grimRNAi.RHG.UAS, hidRNAi.RHG.UAS, rprRNAi.RHG.UAS has decreased cell number | adult stage phenotype
Scer\GAL4ap-md544, grimRNAi.RHG.UAS, hidRNAi.RHG.UAS, rprRNAi.RHG.UAS has visible | adult stage phenotype
Scer\GAL4C10, grimRNAi.RHG.UAS, hidRNAi.RHG.UAS, rprRNAi.RHG.UAS has visible | adult stage phenotype
rprRNAi.RHG.UAS, grimRNAi.RHG.UAS, hidRNAi.RHG.UAS, Scer\GAL4esg.PU is a suppressor of adult gut phenotype of Scer\GAL4esg.PU, synrUAS.ORF.GW.Tag:HA
Scer\GAL4hh.PU, rprRNAi.RHG.UAS, grimRNAi.RHG.UAS, hidRNAi.RHG.UAS is a suppressor of wing disc | third instar larval stage phenotype of Scer\GAL4hh.PU, rlGD4697
rprRNAi.RHG.UAS, grimRNAi.RHG.UAS, hidRNAi.RHG.UAS, Scer\GAL4GMR.PF is a suppressor of eye phenotype of hidGMR.PU
Scer\GAL4nub-AC-62, grimRNAi.RHG.UAS, hepCA.UAS, hidRNAi.RHG.UAS, rprRNAi.RHG.UAS has wing pouch phenotype
Scer\GAL4C10, grimRNAi.RHG.UAS, hidRNAi.RHG.UAS, rprRNAi.RHG.UAS has metatarsus phenotype
Scer\GAL4ap-md544, grimRNAi.RHG.UAS, hidRNAi.RHG.UAS, rprRNAi.RHG.UAS has wing phenotype
Scer\GAL4C10, grimRNAi.RHG.UAS, hidRNAi.RHG.UAS, rprRNAi.RHG.UAS has wing phenotype
Scer\GAL4C10, grimRNAi.RHG.UAS, hidRNAi.RHG.UAS, rprRNAi.RHG.UAS has femur phenotype
Scer\GAL4C10, grimRNAi.RHG.UAS, hidRNAi.RHG.UAS, rprRNAi.RHG.UAS has wing disc phenotype
Scer\GAL4wor.PA, grimRNAi.RHG.UAS, hidRNAi.RHG.UAS, rprRNAi.RHG.UAS has adult mushroom body phenotype
Scer\GAL4wor.PA, grimRNAi.RHG.UAS, hidRNAi.RHG.UAS, rprRNAi.RHG.UAS has neuroblast | ectopic phenotype
Scer\GAL4wor.PA, foxo25, grimRNAi.RHG.UAS, hidRNAi.RHG.UAS, rprRNAi.RHG.UAS has adult mushroom body phenotype
Scer\GAL4wor.PA, foxo25, grimRNAi.RHG.UAS, hidRNAi.RHG.UAS, rprRNAi.RHG.UAS has neuroblast | ectopic phenotype
Atg1K38Q.UAS, Scer\GAL4wor.PA, grimRNAi.RHG.UAS, hidRNAi.RHG.UAS, rprRNAi.RHG.UAS has adult mushroom body phenotype
Atg1K38Q.UAS, Scer\GAL4wor.PA, grimRNAi.RHG.UAS, hidRNAi.RHG.UAS, rprRNAi.RHG.UAS has neuroblast | ectopic phenotype
The triple expression of grimmiRNA.RHG.UAS, hidmiRNA.RHG.UAS and rprmiRNA.RHG.UAS under the control of Scer\GAL4C10 induces significantly smaller wings, with significantly fewer cells, although slightly bigger, as compared to controls. Likewise, the legs are significantly smaller than controls (femur and tarsal segment 1, but not tibia). Wing discs show a significant decrease in apoptosis (detected by PARP:VENUS signal and TUNEL assay), as compared to controls.
The triple expression under the control of Scer\GAL4ap-md544 leads to opaque and curly wings, which indicate apoptosis and growth inhibition during development, respectively.
The eye defect caused by WGMR.PU is effectively rescued by expression of P{UAS-RHG.miRNA} (carries WmiRNA.RHG.Scer\UAS, grimmiRNA.RHG.Scer\UAS and rprmiRNA.RHG.Scer\UAS) under the control of Scer\GAL4GMR.PF.
Mushroom body neuroblasts are seen in the brains of young adults simultaneously co-expressing rprmiRNA.RHG.Scer\UAS, WmiRNA.RHG.Scer\UAS and grimmiRNA.RHG.Scer\UAS (from the P{UAS-RHG.miRNA} transgene) under the control of Scer\GAL4wor.PA, although their persistence is only transient (mushroom body neuroblasts are not seen in wild-type adults).
Neuroblasts are detected in the mushroom body of 2 week old and even in 1 month old adults simultaneously co-expressing rprmiRNA.RHG.Scer\UAS, WmiRNA.RHG.Scer\UAS and grimmiRNA.RHG.Scer\UAS (from the P{UAS-RHG.miRNA} transgene) under the control of Scer\GAL4wor.PA in a foxo25 background (mushroom body neuroblasts are not seen in wild-type adults and are not seen at these late time points in adults expressing the P{UAS-RHG.miRNA} transgene under the control of Scer\GAL4wor.PA in a wild-type background or in foxo25 single mutant adults). Some of the 2 week old mutant mushroom body neuroblasts are large in size and generate many new progeny. There is a strong correlation between mushroom body neuroblast cell size and progeny number in 2 week old mutant adults, but not in younger mutant adults. In 1 month old mutant adults, some of the progeny of these neuroblasts show normal axon projections through the mushroom body pedunculus and some mistarget, bifurcating prematurely and projecting anterior to the pedunculus.
Neuroblasts are detected in the mushroom body of 1 month old adults simultaneously co-expressing rprmiRNA.RHG.Scer\UAS, WmiRNA.RHG.Scer\UAS and grimmiRNA.RHG.Scer\UAS (from the P{UAS-RHG.miRNA} transgene) and Atg1K38Q.Scer\UAS under the control of Scer\GAL4wor.PA (mushroom body neuroblasts are not seen in wild-type adults).