FlyBase Allele Report: Dmel\Past1[60-4]

General Information

Symbol

Dmel\Past1^60-4

Species

D. melanogaster

Name

FlyBase ID

FBal0245164

Feature type

allele

Associated gene

Dmel\Past1

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

Mutagen

Delta2-3 transposase

(Olswang-Kutz et al., 2009)

Progenitor genotype

P{EPgy2}Past1^EY01852

(Olswang-Kutz et al., 2009)

Cytology

Description

Imprecise excision of the progenitor insertion, P{EPgy2}Past1^EY01852, resulting in a 1463 bp deletion 3' to the insertion site, disrupting the second exon of mRNA splice variant A, and the first exon of mRNA splice variant B.

(Olswang-Kutz et al., 2009)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3R:12,697,879..12,699,342 [+]

Comment:

A 1463bp deletion resulting from imprecise excision of P{EPgy2}Past1^EY01852.

(Olswang-Kutz et al., 2009)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate

(Olswang-Kutz et al., 2009)

abnormal neuroanatomy | third instar larval stage (with Past1^110-1)

(Koles et al., 2015)

abnormal neurophysiology | third instar larval stage (with Past1^110-1)

(Koles et al., 2015)

partially lethal - majority die | heat sensitive

(Olswang-Kutz et al., 2009)

semi-sterile

(Olswang-Kutz et al., 2009)

short lived

(Olswang-Kutz et al., 2009)

Phenotype Manifest In

cystic bulge (with Past1^110-1)

(Olswang-Kutz et al., 2009)

egg chamber

(Olswang-Kutz et al., 2009)

egg chamber (with Past1^110-1)

(Olswang-Kutz et al., 2009)

embryonic/larval neuromuscular junction | third instar larval stage (with Past1^110-1)

(Koles et al., 2015)

individualization stage spermatid (with Past1^110-1)

(Olswang-Kutz et al., 2009)

oocyte

(Olswang-Kutz et al., 2009)

oocyte (with Past1^110-1)

(Olswang-Kutz et al., 2009)

ovary

(Olswang-Kutz et al., 2009)

ovary (with Past1^110-1)

(Olswang-Kutz et al., 2009)

spermatid cyst (with Past1^110-1)

(Olswang-Kutz et al., 2009)

subsynaptic reticulum | third instar larval stage (with Past1^110-1)

(Koles et al., 2015)

testis (with Past1^110-1)

(Olswang-Kutz et al., 2009)

type I bouton | third instar larval stage (with Past1^110-1)

(Koles et al., 2015)

Detailed Description

Statement

Reference

Total bouton numbers (type I) at the neuromuscular junction (NMJ) in Past1^60-4/Past1^110-1 third instar larvae are not significantly different to wild type, but show defects in synaptic bouton shape (less round, often with ragged edges); mutants show a significant increase in ghost boutons (muscle 6/7, segment A3) compared to wild type. Despite reduced GluRIIA levels in Past1^60-4/Past1^110-1 larval NMJs, clusters appose normally to presynaptic active zones.

Past1^60-4/Past1^110-1 larval NMJs show significant reductions in the amplitude and frequency of spontaneous miniature excitatory postsynaptic potentials (mEPSPs) as well as significantly dampened evoked postsynaptic potentials (EPSPs); quantal content is not increased to compensate for reduced mEPSPs (rather, it is significantly decreased).

Subsynaptic reticulum (SSR) in Past1^60-4/Past1^110-1 third instar larval NMJs have a similar recovery rate to wild type after photobleaching boutons, but have a significantly higher mobile fraction (SSR may have reduced complexity in mutants). Mutant 1b boutons show normal presynaptic structures (including synaptic vesicles and active zones) but have aberrant SSR, often separated into distinct nodules with a central core surrounded by long membrane sheets (not seen in wild type).

(Koles et al., 2015)

Past1^60-4 homozygous mutants develop 20% more slowly than heterozygotes. Animals reach adulthood but die three to five days after eclosion, whereas sibling controls survive for over a month.

Past1^60-4 animals are sensitive to high temperatures, and survival is impaired at 29[o]C in larvae and adults.

Fertility is much reduced in Past1^60-4 animals. The number of offspring from Past1^60-4 animals mated with wild-type animals is drastically lower compared to controls.

Past1^60-4/Past1^110-1 males have much thinner testes compared to wildtype. There are significantly fewer bundles of elongating and elongated cysts present. Defects are observed during spermatid individualization, and spermatids fail to extrude their entire cytoplasm into a waste bags.

Ovaries from Past1^60-4/Past1^110-1 or Past1^60-4/Past1^60-4 mated females are much smaller, less organised, and contain few well differentiated oocytes (stages 12-14) compared to ovaries from wildtype or heterozygous mated females. The number of egg chambers is reduced compared to controls. Mutant oocytes from stage 9 onwards are devoid of yolk.

(Olswang-Kutz et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Past1^60-4 has partially lethal - majority die phenotype, enhanceable by N^nd-1

(Olswang-Kutz et al., 2009)

Past1^60-4 has short lived phenotype, enhanceable by N^nd-1

(Olswang-Kutz et al., 2009)

Other

Statement

Reference

GluRIIA^SP16, Past1^110-1/Past1^60-4 has abnormal neurophysiology | third instar larval stage phenotype

(Koles et al., 2015)

Phenotype Manifest In

Suppressed by

Statement

Reference

Past1^110-1/Past1^60-4 has subsynaptic reticulum | third instar larval stage phenotype, suppressible by Amph²⁶/Amph²⁶

(Koles et al., 2015)

Enhancer of

Statement

Reference

Past1^60-4 is an enhancer of wing margin phenotype of N^nd-1

(Olswang-Kutz et al., 2009)

Suppressor of

Statement

Reference

Past1[+]/Past1^60-4 is a suppressor of wing vein L2 phenotype of H²

(Olswang-Kutz et al., 2009)

Past1[+]/Past1^60-4 is a suppressor of wing vein L4 phenotype of H²

(Olswang-Kutz et al., 2009)

Other

Statement

Reference

GluRIIA^SP16, Past1^110-1/Past1^60-4 has embryonic/larval neuromuscular junction | third instar larval stage phenotype

(Koles et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

GluRIIA^SP16/GluRIIA^SP16;Past1^60-4/Past1^110-1 double mutant larvae show further reductions in mEPSP amplitude compared to Past1^60-4/Past1^110-1 mutants (but similar in amplitude to GluRIIA^SP16/GluRIIA^SP16 mutants); the compensation increase in quantal content seen in GluRIIA^SP16/GluRIIA^SP16 mutants is not seen in GluRIIA^SP16/GluRIIA^SP16;Past1^60-4/Past1^110-1 double mutants (they show similar diminished quantal content as seen in Past1^60-4/Past1^110-1 larvae).

Amph²⁶/Amph²⁶ suppresses subsynaptic reticulum defects seen at Past1^60-4/Past1^110-1 larval NMJs.

(Koles et al., 2015)

N^nd-1, Past1^60-4 double homozygous flies reach adulthood at a very low frequency, and die after 1 day. The wings are more severely notched compared to N^nd-1 mutant flies.

Only 6% of H², Past1^60-4 double heterozygous mutant flies show defects in wing veins, much reduced compared to single H² mutant flies.

(Olswang-Kutz et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Past1^110-1/Past1^60-4 is rescued by Past1^UAS.EGFP/Scer\GAL4^C57

(Koles et al., 2015)

Comments

Expression of Past1^{Scer\UAS.T:Avic\GFP-EGFP} driven in muscles by Scer\GAL4^C57 rescues NMJ morphology and ghost bouton phenotype in Past1^60-4/Past1^110-1 third instar larvae.

(Koles et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

79617

w^*; sna^Sco/CyO; Past1^60-4/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Past1^60-4

(Olswang-Kutz et al., 2009)

past1^60-4

(Martin et al., 2024, Koles et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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