FlyBase Allele Report: Dmel\H[2]

General Information

Symbol

Dmel\H²

Species

D. melanogaster

Name

FlyBase ID

FBal0005293

Feature type

allele

Associated gene

Dmel\H

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

hypomorphic allele - genetic evidence

loss of function allele

amorphic allele - genetic evidence

Mutagen

spontaneous

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Bang et al., 1991, de la Concha et al., 1988)

hypomorphic allele - genetic evidence

(de Celis et al., 1991)

loss of function allele

(Apidianakis et al., 1999)

Mutagen

spontaneous

(Maier et al., 1992, Bang et al., 1991, Ashburner, 1982)

Progenitor genotype

Cytology

Polytene chromosomes normal.

(Bang et al., 1991)

Description

6.9kb insertion (identity unknown) very close to the site of the hobo insertion in H^B8.

(Maier et al., 1992)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Schimke immuno-osseous dysplasia

modeled by Marcal1^UAS.cBa

(Morimoto et al., 2016)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

eo sensory structure

(Tang et al., 2005)

intestinal stem cell | somatic clone

(Bardin et al., 2010)

macrochaeta

(Tang et al., 2005, Escudero et al., 2003)

macrochaeta & head

(Schweisguth and Posakony, 1992)

macrochaeta & scutum

(Schweisguth and Posakony, 1992)

mechanosensory chaeta | adult stage

(Shukla et al., 2017)

neuron

(Sturtevant and Bier, 1995)

scutellar bristle

(Apidianakis et al., 1999)

sense organ

(Sotillos et al., 1997)

wing

(Djiane et al., 2013)

wing | adult stage

(Slaninova et al., 2016)

wing vein

(Sotillos et al., 1997)

wing vein L2

(Olswang-Kutz et al., 2009)

wing vein L4

(Olswang-Kutz et al., 2009, Marenda et al., 2004)

wing vein L5

(Horvath et al., 2016, de Celis et al., 1997, Sotillos et al., 1997)

Detailed Description

Statement

Reference

H² heterozygous adults display loss of one or more thoracic sensory bristles, as compared o controls.

(Shukla et al., 2017)

H² heterozygotes show a partial loss of wing vein L5

(Horvath et al., 2016)

The wings of H²/+ mutant adults are significantly larger than those of wild type flies.

(Slaninova et al., 2016)

H² wings are larger than wild type.

(Djiane et al., 2013)

Homozygous intestinal stem cell clones lose self-renewing capacity over time, and fail to form large clones at 14 days after clone induction, in contrast to wild-type controls.

(Bardin et al., 2010)

36% of H² heterozygous mutant flies display shortened L2 and L4 longitudinal wing veins.

(Olswang-Kutz et al., 2009)

Flies heterozygous for H² exhibit defects in sensory organ formation. On the notum, approximately 17% of the hairs are absent. This is due to increased N-signaling causing a failure in SOP cell specification or transformation of the hair daughter cell into an extra socket cell.

(Tang et al., 2005)

Heterozygotes show a weak suppression of notum macrochaetae and an essentially normal pattern of microchaetae.

(Escudero et al., 2003)

H¹/H² flies produce nota that are bald.

(Apidianakis et al., 1999)

Homozygotes lack most sensory organs and wing veins. Heterozygotes lack a few sensory organs and the distal part of wing vein L5.

(Sotillos et al., 1997)

Dorsal mitotic clone in the wing reduces vein differentiation in wing vein LV.

(de Celis et al., 1997)

Loss of neuron mutant.

(Sturtevant and Bier, 1995)

Lethal phase is at the transition from 1st to 2nd instar, and larval development preceding lethality is slightly delayed. Heteroallelic combinations with other alleles can allow development of pharate adults, therefore this allele is not considered as a strong allele, as was previously published (Bang et al., Development 111:89-104 ).

(Maier et al., 1992)

Bristle double socket phenotype.

(Schweisguth and Posakony, 1992)

Recessive phenotypic effects. Bristle loss on head and notum and double socket phenotype.

(Bang et al., 1991)

Haplo-insufficient.

(de Celis et al., 1991)

Terminal gaps in LIV and LV and occasionally short terminal gaps in LII and LIII.

(Diaz-Benjumea and Garcia-Bellido, 1990)

Causes reduction of neural hyperplasia due to homozygosity for neur^- and mam^- alleles.

(de la Concha et al., 1988)

Bristle effect more extreme than in H¹ and more easily separated from wild type. Venation effect slighter than in H¹.

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

H² has visible | adult stage phenotype, enhanceable by Sirt1[+]/Sirt1^2A-7-11

(Horvath et al., 2016)

H² has visible | adult stage phenotype, enhanceable by Sirt1^2A-7-11/Sirt1^2A-7-11

(Horvath et al., 2016)

H² has abnormal size phenotype, enhanceable by Df(3R)Exel9019/+

(Djiane et al., 2013)

H² has abnormal size phenotype, enhanceable by +/Df(3L)Exel9001

(Djiane et al., 2013)

NOT Enhanced by

Statement

Reference

H² has abnormal size phenotype, non-enhanceable by Df(2L)BSC251/+

(Djiane et al., 2013)

H² has abnormal size phenotype, non-enhanceable by Df(2R)BSC651/+

(Djiane et al., 2013)

H² has abnormal size phenotype, non-enhanceable by Df(2R)BSC274/+

(Djiane et al., 2013)

H² has abnormal size phenotype, non-enhanceable by Df(2L)Exel6005/+

(Djiane et al., 2013)

H² has abnormal size phenotype, non-enhanceable by Df(2L)BSC255/+

(Djiane et al., 2013)

H² has abnormal size phenotype, non-enhanceable by Df(2L)BSC184/+

(Djiane et al., 2013)

H² has abnormal size phenotype, non-enhanceable by Df(2L)Exel8034/+

(Djiane et al., 2013)

H² has abnormal size phenotype, non-enhanceable by Df(2R)BSC337/+

(Djiane et al., 2013)

H² has abnormal size phenotype, non-enhanceable by Df(3R)Exel6179/+

(Djiane et al., 2013)

H² has abnormal size phenotype, non-enhanceable by Df(2R)BSC660/+

(Djiane et al., 2013)

H² has abnormal size phenotype, non-enhanceable by Df(2R)BSC668/+

(Djiane et al., 2013)

H² has abnormal size phenotype, non-enhanceable by Df(2R)ED2076/+

(Djiane et al., 2013)

H² has abnormal size phenotype, non-enhanceable by Df(3L)BSC773/+

(Djiane et al., 2013)

H² has abnormal size phenotype, non-enhanceable by Df(2L)BSC226/+

(Djiane et al., 2013)

H² has abnormal size phenotype, non-enhanceable by Df(1)BSC535/+

(Djiane et al., 2013)

H² has abnormal size phenotype, non-enhanceable by Df(3R)Exel6212/+

(Djiane et al., 2013)

H² has abnormal size phenotype, non-enhanceable by +/Df(2R)Exel7123

(Djiane et al., 2013)

H² has abnormal size phenotype, non-enhanceable by Df(2L)Exel6028/+

(Djiane et al., 2013)

Suppressed by

Statement

Reference

H² has abnormal size phenotype, suppressible by Df(2L)BSC200/+

(Djiane et al., 2013)

H² has abnormal size phenotype, suppressible by Df(1)Exel6233/+

(Djiane et al., 2013)

H² has abnormal size phenotype, suppressible by Df(1)Exel6251/+

(Djiane et al., 2013)

H² has abnormal size phenotype, suppressible by Df(1)BSC352/+

(Djiane et al., 2013)

H² has abnormal size phenotype, suppressible by Df(1)BSC870/+

(Djiane et al., 2013)

H² has visible | dominant phenotype, suppressible by dx²⁴

(Nagel et al., 2000)

H² has visible phenotype, suppressible by kuz^k01405

(Sotillos et al., 1997)

NOT suppressed by

Statement

Reference

H² has abnormal size phenotype, non-suppressible by Df(2R)BSC337/+

(Djiane et al., 2013)

H² has abnormal size phenotype, non-suppressible by Df(3R)Exel6179/+

(Djiane et al., 2013)

H² has abnormal size phenotype, non-suppressible by Df(2R)BSC660/+

(Djiane et al., 2013)

H² has abnormal size phenotype, non-suppressible by Df(2R)BSC668/+

(Djiane et al., 2013)

H² has abnormal size phenotype, non-suppressible by Df(2R)ED2076/+

(Djiane et al., 2013)

H² has abnormal size phenotype, non-suppressible by Df(3L)BSC773/+

(Djiane et al., 2013)

H² has abnormal size phenotype, non-suppressible by Df(2L)BSC226/+

(Djiane et al., 2013)

H² has abnormal size phenotype, non-suppressible by Df(1)BSC535/+

(Djiane et al., 2013)

H² has abnormal size phenotype, non-suppressible by Df(3R)Exel6212/+

(Djiane et al., 2013)

H² has abnormal size phenotype, non-suppressible by +/Df(2R)Exel7123

(Djiane et al., 2013)

H² has abnormal size phenotype, non-suppressible by Df(2L)Exel6028/+

(Djiane et al., 2013)

H² has abnormal size phenotype, non-suppressible by Df(2L)BSC251/+

(Djiane et al., 2013)

H² has abnormal size phenotype, non-suppressible by Df(2R)BSC651/+

(Djiane et al., 2013)

H² has abnormal size phenotype, non-suppressible by Df(2R)BSC274/+

(Djiane et al., 2013)

H² has abnormal size phenotype, non-suppressible by Df(2L)Exel6005/+

(Djiane et al., 2013)

H² has abnormal size phenotype, non-suppressible by Df(2L)BSC255/+

(Djiane et al., 2013)

H² has abnormal size phenotype, non-suppressible by Df(2L)BSC184/+

(Djiane et al., 2013)

H² has abnormal size phenotype, non-suppressible by Df(2L)Exel8034/+

(Djiane et al., 2013)

NOT Enhancer of

Statement

Reference

H² is a non-enhancer of visible phenotype of upd1^GMR.PB

(Bach et al., 2003)

Suppressor of

Statement

Reference

H²/H[+] is a suppressor | partially of visible phenotype of ed^slH8/ed^1X5

(Escudero et al., 2003)

H²/H[+] is a suppressor of visible | dominant phenotype of amos^Tft

(Villa-Cuesta et al., 2003)

H²/H[+] is a suppressor of visible | dominant phenotype of dx²⁴

(Nagel et al., 2000)

H² is a suppressor of visible phenotype of Scer\GAL4^455.2, l(1)sc^UAS.cHa

(Rottgen et al., 1998)

H² is a suppressor of visible | recessive phenotype of kuz^k01405

(Sotillos et al., 1997)

H² is a suppressor of FlyBase_internaltransgene_features:cell lethal:cell lethal | somatic clone phenotype of Delta^9P

(Dietrich and Campos-Ortega, 1984)

NOT Suppressor of

Statement

Reference

H² is a non-suppressor of visible phenotype of upd1^GMR.PB

(Bach et al., 2003)

Phenotype Manifest In

Enhanced by

Statement

Reference

H² has mechanosensory chaeta | adult stage phenotype, enhanceable by Rbfox1^RNAi.UAS/Scer\GAL4^sca-109-68

(Shukla et al., 2017)

H² has wing vein L5 phenotype, enhanceable by Sirt1[+]/Sirt1^2A-7-11

(Horvath et al., 2016)

H² has wing vein L5 phenotype, enhanceable by Sirt1^2A-7-11/Sirt1^2A-7-11

(Horvath et al., 2016)

H² has macrochaeta phenotype, enhanceable by Eps-15^EP2513/Eps-15²

(Tang et al., 2005)

H² has eo sensory structure phenotype, enhanceable by Eps-15^EP2513/Eps-15²

(Tang et al., 2005)

H² has phenotype, enhanceable by Su(H)^+t6.0

(Schweisguth and Posakony, 1992)

H² has phenotype, enhanceable by Egfr^t1/Egfr^f11

(Diaz-Benjumea and Garcia-Bellido, 1990)

Suppressed by

Statement

Reference

H² has wing vein L2 phenotype, suppressible by Past1[+]/Past1^60-4

(Olswang-Kutz et al., 2009)

H² has wing vein L4 phenotype, suppressible by Past1[+]/Past1^60-4

(Olswang-Kutz et al., 2009)

H² has wing vein L4 phenotype, suppressible by Snr1^E1

(Marenda et al., 2004)

H² has wing vein phenotype, suppressible by dx²⁴

(Nagel et al., 2000)

H² has sense organ phenotype, suppressible by kuz^k01405

(Sotillos et al., 1997)

H² has phenotype, suppressible by Su(H)⁸

(Schweisguth and Posakony, 1992)

H² has phenotype, suppressible by Egfr^E3/Egfr[+]

(Diaz-Benjumea and Garcia-Bellido, 1990)

NOT suppressed by

Statement

Reference

H² has wing vein phenotype, non-suppressible by kuz^k01405

(Sotillos et al., 1997)

Enhancer of

Statement

Reference

H²/H[+] is an enhancer of phenotype of Egfr^t1/Egfr^f11

(Diaz-Benjumea and Garcia-Bellido, 1990)

NOT Enhancer of

Statement

Reference

H² is a non-enhancer of eye phenotype of upd1^GMR.PB

(Bach et al., 2003)

Suppressor of

Statement

Reference

H²/H[+] is a suppressor of macrochaeta | ectopic phenotype of ed^slH8/ed^1X5

(Escudero et al., 2003)

H²/H[+] is a suppressor of macrochaeta | ectopic phenotype of amos^Tft

(Villa-Cuesta et al., 2003)

H²/H[+] is a suppressor of wing vein phenotype of dx²⁴

(Nagel et al., 2000)

H²/H[+] is a suppressor of wing phenotype of dx²⁴

(Nagel et al., 2000)

H² is a suppressor of scutellar bristle phenotype of E(spl)m4-BFM^UAS.cAa, Scer\GAL4^ptc-559.1

(Apidianakis et al., 1999)

H²/H[+] is a suppressor of phenotype of Hsc70-4¹⁹⁵

(Hing et al., 1999)

H² is a suppressor of scutellar bristle phenotype of Scer\GAL4^455.2, l(1)sc^UAS.cHa

(Rottgen et al., 1998)

H² is a suppressor of wing phenotype of kuz^k01405

(Sotillos et al., 1997)

H²/H[+] is a suppressor of phenotype of Egfr^E3

(Diaz-Benjumea and Garcia-Bellido, 1990)

H² is a suppressor of eye | somatic clone phenotype of Delta^9P

(Dietrich and Campos-Ortega, 1984)

H² is a suppressor of presumptive embryonic/larval central nervous system phenotype of Delta^9P

(Dietrich and Campos-Ortega, 1984)

H² is a suppressor of eye | somatic clone phenotype of neur¹¹

(Dietrich and Campos-Ortega, 1984)

NOT Suppressor of

Statement

Reference

H² is a non-suppressor of eye phenotype of upd1^GMR.PB

(Bach et al., 2003)

H²/H[+] is a non-suppressor of microchaeta phenotype of ed^slH8/ed^1X5

(Escudero et al., 2003)

H²/H[+] is a non-suppressor of wing phenotype of dx²⁴

(Nagel et al., 2000)

Other

Statement

Reference

H², Rbfox1^RNAi.UAS, Scer\GAL4^sca-109-68 has tormogen cell | adult stage phenotype

(Shukla et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

H² heterozygous adults also expressing Rbfox1^{dsRNA.Scer\UAS} under the control of Scer\GAL4^sca-109-68 exhibit an enhanced loss of thoracic sensory bristles and exhibit a significant amount of sockets instead of bristles, as compared to H² heterozygotes.

(Shukla et al., 2017)

The shortening of the wing vein L5 observed H² heterozygous adults is partially rescued by combination with one or (more strongly) two copies of Sirt1^2A-7-11.

(Horvath et al., 2016)

Only 6% of H², Past1^60-4 double heterozygous mutant flies show defects in wing veins, much reduced compared to single H² mutant flies.

Only 6% of H², Past1^110-1 double heterozygous mutant flies show defects in wing veins, much reduced compared to single H² mutant flies.

(Olswang-Kutz et al., 2009)

A Eps-15²/Eps-15^EP2513 transheterozygous background significantly enhances the hair loss phenotype observed in H² heterozygotes. This enhancement is gene dosage dependent with loss of both copies of Eps-15 increasing the hair loss to 46%, accompanied by increases of nearly four-fold in the number of double sockets and nearly two-fold in the number of areas with no external sensory organs.

Su(H)¹ heterozygosity suppresses the hair loss phenotype found in Eps-15²/Eps-15^EP2513; H²/+ mutants.

(Tang et al., 2005)

The incomplete L4 wing vein phenotype seen in H² mutants is suppressed by Snr1^E1.

(Marenda et al., 2004)

H²/+ almost completely eliminates the extra macrochaetae seen on the notum of ed^1X5/ed^slH8 animals, although the increased density of microchaetae seen in ed^1X5/ed^slH8 flies is not reduced by H²/+.

(Escudero et al., 2003)

The average number of ectopic macrochaetae per heminotum in amos^Tft/+ flies is reduced to 19 +/- 2 if they are also carrying H²/+.

(Villa-Cuesta et al., 2003)

Effects on wing development by dx²⁴/Y and H²/+ are mutually levelled. dx²⁴/Y wing phenotypes, both regarding vein broadening and spread out wings, as well as haploinsufficient vein shortening in H² mutants are largely restored in dx²⁴/Y, H²/+ flies. Yet the fragile wing texture typical of dx mutants remains.

(Nagel et al., 2000)

The presence of H² suppresses the supernumerary phenotype seen in flies with m4^Scer\UAS.cAa driven by Scer\GAL4^ptc-559.1.

(Apidianakis et al., 1999)

Mutation suppresses the ectopic scutellar bristle phenotype seen from Scer\GAL4^455.2-mediated expression of l(1)sc^Scer\UAS.cHa.

(Rottgen et al., 1998)

The sensory organ but not the wing vein phenotypes of H² are suppressed by kuz^k01405. The neurogenic phenotype and reduced wing size of kuz^k01405 homozygotes is suppressed by H².

(Sotillos et al., 1997)

Phenotype can be suppressed by Su(H)⁸, alleviated by introduction of P{Su(H)⁺6.0} into H² Su(H)⁸ flies and enhanced by a copy of P{Su(H)⁺6.0} introduced into heterozygotes.

(Schweisguth and Posakony, 1992)

Egfr^t1/Egfr^f11 H²/+ double mutants have a superadditive phenotype. Egfr^E3/+ shows a negative interaction in combination with H²/+.

(Diaz-Benjumea and Garcia-Bellido, 1990)

H² reduces the central nervous system hypertrophy seen in Dl^9P homozygous embryos. Double homozygous Dl^9P H² clones in the eye are viable, and are sometimes indistinguishable from wild-type. H² reduces the severity of the phenotype of neur¹¹ clones in the eye.

(Dietrich and Campos-Ortega, 1984)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (7)

517

107696

105957

H²/T(2;3)ap^Xa, ap^Xa

101647

H² / Tp(3;3)Abd-B^Vno, Vno¹

106105

Tp(3;3)Vno, Abd-B^Vno, Vno¹/H²

107695

Ki¹ rsd¹ Ubx¹ H² Dr¹/TM6B, Tb¹

106008

st¹ sr¹ H² ca¹/In(3R)P, st¹ l(3)W¹ ca¹

Notes on Origin

Discoverer

Sturtevant.

Comments

Strong H allele.

(Bang et al., 1991)

Heteroallelic combinations with other alleles can allow development of pharate adults, therefore this allele is not considered as a strong allele, as was previously published (Bang et al., Development 111:89-104 ). Phenotypic series of H alleles, progressing from strongest (amorphic) to weakest mutant phenotype: H^B79 = H⁸¹ >> H¹ = H⁹⁹ > H^bob > H^94a > H^88d > H^B8 > H² >= H³ > H^A120.

(Maier et al., 1992)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

H²

(Viswanathan et al., 2021, Shukla et al., 2017, Zhu et al., 2017, Horvath et al., 2016, Morimoto et al., 2016, Praxenthaler et al., 2015, Djiane et al., 2013, Duan et al., 2011, Bardin et al., 2010, Olswang-Kutz et al., 2009, Tang et al., 2005)

Name Synonyms

Secondary FlyBase IDs

References (45)

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