FlyBase Allele Report: Dmel\Rbfox1[RNAi.UAS]

The expression of Rbfox1^{dsRNA.Scer\UAS} under the control of Scer\GAL4^sca-109-68 results in adults presenting supernumerary thoracic sensory bristles and presenting an increased number of wing campaniform sensilla - i.e. L3 vein sensilla and wing margin twin sensilla -, which are also frequently transformed into bristle structures, and results in third instar larval wing discs presenting an increased number of sensory organ precursor cells but no obvious proneural cluster size changes, as compared to controls. Expression under the control of Scer\GAL4^sca-C253 results in adults presenting supernumerary thoracic sensory bristles and results in third instar larval wing discs presenting an increased number of sensory organ precursor cells, as compared to controls. Expression under the control of Scer\GAL4^sca-109-68, Scer\GAL4^pnr-MD237, Scer\GAL4^MS248 or Scer\GAL4^ap.PU, but not Scer\GAL4^pros.PMG, results in adults presenting supernumerary thoracic sensory bristles, as compared to controls.

(Shukla et al., 2017)

Expression of A2bp1^{dsRNA.Scer\UAS} using Scer\GAL4^tub or Scer\GAL4^hs.PB causes early larval lethality.

Expression of A2bp1^{dsRNA.Scer\UAS} using Scer\GAL4^{A2bp1-EN403-GAL4} results in loss of intervein region and ectopic cross veins between L3 and L4 of the adult wing blade.

Expression of A2bp1^{dsRNA.Scer\UAS} using Scer\GAL4^bbg-C96 or Scer\GAL4^vg.PM does not result in any wing phenotype and the flies are fully viable.

Expression of A2bp1^{dsRNA.Scer\UAS} using Scer\GAL4^bi-md653, Scer\GAL4^ap-md544, Scer\GAL4^Bx-MS1096 or Scer\GAL4^ptc-559.1 causes loss of intervein region and fusion of veins L3 and L4. In some cases, there is an excess of vein tissue.

Clonal induction of A2bp1^{dsRNA.Scer\UAS} causes ectopic veins in the wing blade, with the majority being between veins L3 and L4.

Expression of A2bp1^{dsRNA.Scer\UAS} using Scer\GAL4^Bx-MS1096 in the presence of one copy of A2bp1^EY01049 causes an enhanced L3/L4 vein phenotype: fusion of veins L3 and L4 is accompanied by thickening of the fused vein, and large patches of ectopic veins are observed in regions beyond L5.

(Usha and Shashidhara, 2010)

External Data

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Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Rbfox1^RNAi.UAS, Scer\GAL4^sca-C253 is an enhancer of increased cell number | third instar larval stage phenotype of N^DN.UAS, Scer\GAL4^sca-C253

(Shukla et al., 2017)

Other

Statement

Reference

Dcr-2^UAS.cDa, Rbfox1^RNAi.UAS, Scer\GAL4^Bx-MS1096 has lethal | pupal stage phenotype

(Usha and Shashidhara, 2010)

Phenotype Manifest In

Enhanced by

Statement

Reference

Rbfox1^RNAi.UAS, Scer\GAL4^sca-109-68 has mechanosensory chaeta | adult stage | increased number phenotype, enhanceable by N[+]/N^55e11

(Shukla et al., 2017)

Rbfox1^RNAi.UAS, Scer\GAL4^sca-C253 has mechanosensory chaeta | adult stage | increased number phenotype, enhanceable by N^DN.UAS, Scer\GAL4^sca-C253

(Shukla et al., 2017)

Rbfox1^RNAi.UAS, Scer\GAL4^sca-109-68 has mechanosensory chaeta | adult stage | increased number phenotype, enhanceable by spdo^UAS.cOa, Scer\GAL4^sca-109-68

(Shukla et al., 2017)

NOT Enhanced by

Statement

Reference

Rbfox1^RNAi.UAS, Scer\GAL4^Mef2.PR has abdomen phenotype, non-enhanceable by salm^GD1525, Scer\GAL4^Mef2.PR

(Nikonova et al., 2022)

Rbfox1^RNAi.UAS, Scer\GAL4^Mef2.PR has tergotrochanter muscle cell phenotype, non-enhanceable by salm^GD1525, Scer\GAL4^Mef2.PR

(Nikonova et al., 2022)

Suppressed by

Statement

Reference

Rbfox1^RNAi.UAS, Scer\GAL4^Mef2.PR has sarcomere phenotype, suppressible by Mhc^2B

(Nikonova et al., 2022)

Rbfox1^RNAi.UAS, Scer\GAL4^Mef2.PR has indirect flight muscle cell phenotype, suppressible by Mhc^2B

(Nikonova et al., 2022)

Rbfox1^RNAi.UAS, Scer\GAL4^Mef2.PR has muscle cell | decreased number phenotype, suppressible by Mhc^2B

(Nikonova et al., 2022)

Rbfox1^RNAi.UAS, Scer\GAL4^Mef2.PR has indirect flight muscle cell phenotype, suppressible | partially by wupA^hdp-3

(Nikonova et al., 2022)

Rbfox1^RNAi.UAS, Scer\GAL4^Mef2.PR has muscle cell | decreased number phenotype, suppressible | partially by wupA^hdp-3

(Nikonova et al., 2022)

Rbfox1^RNAi.UAS, Scer\GAL4^Mef2.PR has dorsal longitudinal indirect flight muscle cell phenotype, suppressible by Mhc^2B

(Nikonova et al., 2022)

Rbfox1^RNAi.UAS, Scer\GAL4^Mef2.PR has dorsal longitudinal indirect flight muscle cell phenotype, suppressible | partially by wupA^hdp-3

(Nikonova et al., 2022)

Rbfox1^RNAi.UAS, Scer\GAL4^sca-109-68 has mechanosensory chaeta | increased number | adult stage phenotype, suppressible | partially by sens^E2/sens[+]

(Shukla et al., 2017)

Rbfox1^RNAi.UAS, Scer\GAL4^sca-109-68 has mechanosensory chaeta | increased number | adult stage phenotype, suppressible | partially by sens^E58/sens[+]

(Shukla et al., 2017)

Rbfox1^RNAi.UAS, Scer\GAL4^bi-md653 has wing cell phenotype, suppressible by kn^UAS.cMa, Scer\GAL4^bi-md653

(Usha and Shashidhara, 2010)

NOT suppressed by

Statement

Reference

Rbfox1^RNAi.UAS, Scer\GAL4^Mef2.PR has indirect flight muscle cell phenotype, non-suppressible by wupA^fliH

(Nikonova et al., 2022)

Rbfox1^RNAi.UAS, Scer\GAL4^Mef2.PR has abdomen phenotype, non-suppressible by salm^GD1525, Scer\GAL4^Mef2.PR

(Nikonova et al., 2022)

Rbfox1^RNAi.UAS, Scer\GAL4^Mef2.PR has dorsal longitudinal indirect flight muscle cell phenotype, non-suppressible by wupA^fliH

(Nikonova et al., 2022)

Rbfox1^RNAi.UAS, Scer\GAL4^Mef2.PR has tergotrochanter muscle cell phenotype, non-suppressible by salm^GD1525, Scer\GAL4^Mef2.PR

(Nikonova et al., 2022)

Enhancer of

Statement

Reference

Rbfox1^RNAi.UAS, Scer\GAL4^Mef2.PR is an enhancer of indirect flight muscle cell phenotype of Scer\GAL4^Mef2.PR, salm^GD1525

(Nikonova et al., 2022)

Rbfox1^RNAi.UAS/Scer\GAL4^sca-109-68 is an enhancer of mechanosensory chaeta | adult stage phenotype of H³

(Shukla et al., 2017)

Rbfox1^RNAi.UAS, Scer\GAL4^sca-C253 is an enhancer of mechanosensory chaeta | adult stage | increased number phenotype of N^DN.UAS, Scer\GAL4^sca-C253

(Shukla et al., 2017)

Rbfox1^RNAi.UAS, Scer\GAL4^sca-C253 is an enhancer of external sensory organ precursor cell | third instar larval stage phenotype of N^DN.UAS, Scer\GAL4^sca-C253

(Shukla et al., 2017)

Rbfox1^RNAi.UAS, Scer\GAL4^sca-109-68 is an enhancer of mechanosensory chaeta | adult stage | increased number phenotype of Scer\GAL4^sca-109-68, spdo^UAS.cOa

(Shukla et al., 2017)

Rbfox1^RNAi.UAS/Scer\GAL4^sca-109-68 is an enhancer of mechanosensory chaeta | adult stage phenotype of H¹

(Shukla et al., 2017)

Rbfox1^RNAi.UAS/Scer\GAL4^sca-109-68 is an enhancer of mechanosensory chaeta | adult stage phenotype of H²

(Shukla et al., 2017)

NOT Enhancer of

Statement

Reference

Rbfox1^RNAi.UAS, Scer\GAL4^pros.PMG is a non-enhancer of mechanosensory chaeta | adult stage | increased number phenotype of N^DN.UAS, Scer\GAL4^pros.PMG

(Shukla et al., 2017)

Suppressor of

Statement

Reference

Rbfox1^RNAi.UAS/Scer\GAL4^sca-109-68 is a suppressor of mechanosensory chaeta | adult stage phenotype of N^Ax-16

(Shukla et al., 2017)

NOT Suppressor of

Statement

Reference

Rbfox1^RNAi.UAS, Scer\GAL4^sca-109-68 is a non-suppressor of macrochaeta | adult stage phenotype of E(spl)m8-HLH^UAS.cUa, Scer\GAL4^sca-109-68

(Shukla et al., 2017)

Rbfox1^RNAi.UAS, Scer\GAL4^sca-109-68 is a non-suppressor of microchaeta | adult stage phenotype of E(spl)m8-HLH^UAS.cUa, Scer\GAL4^sca-109-68

(Shukla et al., 2017)

Rbfox1^RNAi.UAS, Scer\GAL4^sca-C253 is a non-suppressor of macrochaeta | adult stage phenotype of N^int.G.UAS, Scer\GAL4^sca-C253

(Shukla et al., 2017)

Rbfox1^RNAi.UAS, Scer\GAL4^sca-C253 is a non-suppressor of microchaeta | adult stage phenotype of N^int.G.UAS, Scer\GAL4^sca-C253

(Shukla et al., 2017)

Rbfox1^RNAi.UAS/Scer\GAL4^sca-109-68 is a non-suppressor of mechanosensory chaeta | increased number | adult stage phenotype of N^55e11

(Shukla et al., 2017)

Other

Statement

Reference

Rbfox1^RNAi.UAS, Scer\GAL4^Mef2.PR, bru1^GD8699 has indirect flight muscle cell phenotype

(Nikonova et al., 2022)

Rbfox1^RNAi.UAS, Scer\GAL4^Mef2.PR, salm^GD1525 has myofibril phenotype

(Nikonova et al., 2022)

Rbfox1^RNAi.UAS, Scer\GAL4^Mef2.PR, salm^GD1525 has tergotrochanter muscle cell phenotype

(Nikonova et al., 2022)

H¹, Rbfox1^RNAi.UAS, Scer\GAL4^sca-109-68 has tormogen cell | adult stage phenotype

(Shukla et al., 2017)

H², Rbfox1^RNAi.UAS, Scer\GAL4^sca-109-68 has tormogen cell | adult stage phenotype

(Shukla et al., 2017)

H³, Rbfox1^RNAi.UAS, Scer\GAL4^sca-109-68 has tormogen cell | adult stage phenotype

(Shukla et al., 2017)

Rbfox1^RNAi.UAS, Scer\GAL4^sca-109-68, spdo^UAS.cOa has external sensory organ | adult stage phenotype

(Shukla et al., 2017)

Rbfox1^RNAi.UAS, Scer\GAL4^bi-md653, kn^UAS.cMa has wing vein phenotype

(Usha and Shashidhara, 2010)

Additional Comments

Genetic Interactions

Statement

Reference

The supernumerary thoracic sensory bristles in individuals expressing Rbfox1^{dsRNA.Scer\UAS} under the control of Scer\GAL4^sca-109-68 is partially suppressed by heterozygosity for either sens^E58 or sens^E2.

The expression of Rbfox1^{dsRNA.Scer\UAS} under the control of Scer\GAL4^sca-109-68 enhances the loss of thoracic sensory bristles and leads to significant amount of sockets instead of bristles in individuals that are heterozygous for H¹, H² or H³, fully suppresses the decreased number of thoracic sensory bristles present in N^Ax-16 heterozygous adults, but does not suppress the complete loss of thoracic sensory macrochaeta and microchaeta that results from the expression of either E(spl)m8-HLH^Scer\UAS.cUa alone or N^{int.G.Scer\UAS} alone under the control of Scer\GAL4^sca-109-68.

The expression of Rbfox1^{dsRNA.Scer\UAS} under the control of Scer\GAL4^sca-109-68 in N^55e11 heterozygotes or in individuals also expressing spdo^Scer\UAS.cOa leads to the strong enhancement of the supernumerary thoracic sensory bristle phenotype observed in individuals only expressing Rbfox1^{dsRNA.Scer\UAS} or spdo^Scer\UAS.cOa under the control of Scer\GAL4^sca-109-68, or in individuals only heterozygous for N^55e11; co-expression of both Rbfox1^{dsRNA.Scer\UAS} and spdo^Scer\UAS.cOa even leads to to an increase in the number of other (i.e. non-bristle) sensory organs in the thorax, as compared to controls; expression of Rbfox1^{dsRNA.Scer\UAS} in N^55e11 heterozygotes even leads to ectopic thoracic bristles, as compared to controls.

The co-expression of both Rbfox1^{dsRNA.Scer\UAS} and N^DN.Scer\UAS strongly enhances both the supernumerary thoracic sensory bristle phenotype in adults (when under the control of Scer\GAL4^sca-C253, but not when under the control of Scer\GAL4^pros.PMG) and the increased number of sensory organ precursor cells in third instar larval wing discs (when under the control of Scer\GAL4^sca-C253) which result from the expression of either N^DN.Scer\UAS alone or Rbfox1^{dsRNA.Scer\UAS} alone.

(Shukla et al., 2017)

Co-expression of kn^Scer\UAS.cMa in a Scer\GAL4^bi-md653, A2bp1^{dsRNA.Scer\UAS} background rescues the loss of intervein tissue phenotype and causes a loss of vein tissue.

Co-expression of Dcr-2^Scer\UAS.cDa in a Scer\GAL4^Bx-MS1096, A2bp1^{dsRNA.Scer\UAS} background enhances the knock-down of A2bp1 and results in death at early pupal stages.

(Usha and Shashidhara, 2010)

Xenogenetic Interactions

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Reference

Complementation and Rescue Data

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Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

A2bp1^{dsRNA.Scer\UAS}

Rbfox1^RNAi.UAS

Rbfox1^{dsRNA.Scer\UAS}

Rbfox1^dsRNA.UAS

Name Synonyms

Secondary FlyBase IDs

References (4)

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