FB2024_03 , released June 25, 2024
Allele: Dmel\MED241
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General Information
Symbol
Dmel\MED241
Species
D. melanogaster
Name
FlyBase ID
FBal0246389
Feature type
allele
Associated gene
Dmel\MED24
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class

amorphic allele - genetic evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - genetic evidence

(Wang et al., 2010)
Mutagen
ethyl methanesulfonate
(Wang et al., 2008)
Progenitor genotype
Cytology
Description

Amino acid replacement: Q212term.

(Wang et al., 2008)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
3L:8,193,860..8,193,860 [+]
Nucleotide change:

C8193860T

Amino acid change:

Q212term | MED24-PA

Reported amino acid change:

Q212term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Wang et al., 2008)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      MED241/Df(3L)Exel6112 animals show normal mid-third instar (wandering behaviour) and late larval (everting anterior spiracles, barrel shape, midgut programmed cell death) ecdysone-triggered responses. The prepupal ecdysone-triggered responses (head eversion, wing and leg inflation and leg extension) also occur normally. In the salivary gland, mid-third instar glue synthesis is normal, late larval glue secretion is normal, but prepupal salivary gland programmed cell death is defective.

      The formation of the large acidic structures which normally occurs in salivary gland cells approximately 1.5 hours after head eversion is blocked in MED241/Df(3L)Exel6112 animals. Caspase activation at 2 hours after head eversion is also blocked. This results in a persistant salivary gland phenotype at 24 hours after puparium formation.

      (Ihry and Bashirullah, 2014)

      42-46% of MED241 pupae at 24 hr APF show persistent larval salivary glands and none or very few eclose. MED241/Df(3L)Exel6112 hemizygotes show similar phenotypes.

      MED241/Df(3L)Exel6112 pupae appear virtually identical to controls at 12 hr APF, with no apparent defects in adult head eversion or leg eversion, and no delay between puparium formation and head eversion. However, whereas control salivary glands rupture and degrade soon after head eversion, mutant glands remain intact and maintain their morphological integrity.

      MED241/Df(3L)Exel6112 salivary glands do not exhibit caspase-driven programmed cell death at 14 hr or 20 hr APF, in contrast to salivary glands from controls. However, salivary gland autophagy is induced normally.

      (Wang et al., 2010)

      Mutants show defects in destruction of the larval salivary glands, having persistant larval salivary glands at the pupal stage.

      (Wang et al., 2008)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT suppressed by
      Statement
      Reference

      MED241 has lethal | pupal stage phenotype, non-suppressible by Scer\GAL4Act5C.PU/Mdh2UAS.cWa

      (Wang et al., 2010)
      Phenotype Manifest In
      NOT suppressed by
      Statement
      Reference

      MED241 has embryonic/larval salivary gland | pupal stage phenotype, non-suppressible by Scer\GAL4Act5C.PU/Mdh2UAS.cWa

      (Wang et al., 2010)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Scer\GAL4Act5C.PU-mediated expression of Mdh2Scer\UAS.cWa fails to restore normal salivary gland cell death or rescue pupal lethality of MED241 animals.

      (Wang et al., 2010)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      MED241 is rescued by MED24UAS.cWa

      (Wang et al., 2010)
      Comments

      Scer\GAL4Act5C.PU-mediated expression of MED24Scer\UAS.cWa restores normal salivary gland cell death and efficiently rescues pupal lethality in MED241 animals.

      (Wang et al., 2010)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (8)
      Reported As
      Symbol Synonym
      9293
      (Wang et al., 2008)
      MED241
      MED249293
      Med241
      (Wang et al., 2010)
      Med249293
      (Wang et al., 2010)
      med241
      (Ihry and Bashirullah, 2014)
      med24psg5b
      (Ihry and Bashirullah, 2014)
      psg5b
      (Ihry and Bashirullah, 2014)
      Name Synonyms
      Secondary FlyBase IDs
        References (3)