FB2024_03 , released June 25, 2024
Allele: Dmel\porinA2
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General Information
Symbol
Dmel\porinA2
Species
D. melanogaster
Name
FlyBase ID
FBal0247214
Feature type
allele
Associated gene
Dmel\porin
Associated Insertion(s)
Carried in Construct
Key Links
Allele class

hypomorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

hypomorphic allele - molecular evidence

(Park et al., 2010)
Mutagen
P-element activity
(Park et al., 2010)
Progenitor genotype
P{EP}G6147
(Park et al., 2010)
Cytology
Description

Imprecise excision of the progenitor insertion, resulting in a 1381bp deletion that removes the start codon and first two exons of porin.

(Park et al., 2010)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Homozygous adults start to show defects in climbing ability and their ability to fly at 20 days of age, and then die within 30 days of eclosion.

      Homozygous males are sterile. No mature sperm are seen in the vas deferens. The early stages of spermatogenesis appear normal but the mutant males show defects in spermatid individualization; the morphology of the cystic bulges and waste bags is abnormal. The nebenkern are less condensed than normal in the mutant spermatids.

      Mutant flies show a similar survival rate as control flies after feeding with 20mM paraquat.

      Mutant eye discs show a similar level of apoptosis following incubation with an ionophore as do control eye discs.

      Homozygous and porinA2/Df(2L)BSC143 adults show defects in mitochondrial morphology in the indirect flight muscles, with elongated tube-like mitochondria being seen between the muscle fibers.

      (Park et al., 2010)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Statement
      Reference

      porinA2 has abnormal flight phenotype, suppressible by Scer\GAL4Mef2.PR/Drp1UAS.Tag:HA

      (Park et al., 2010)
      NOT Suppressor of
      Statement
      Reference

      porin[+]/porinA2 is a non-suppressor of visible phenotype of DebclUAS.Tag:HA, Scer\GAL4GMR.PF

      (Park et al., 2010)

      porin[+]/porinA2 is a non-suppressor of increased cell death phenotype of DebclUAS.Tag:HA, Scer\GAL4GMR.PF

      (Park et al., 2010)

      porinA2/porinA2 is a non-suppressor of visible phenotype of DebclUAS.Tag:HA, Scer\GAL4GMR.PF

      (Park et al., 2010)

      porinA2/porinA2 is a non-suppressor of increased cell death phenotype of DebclUAS.Tag:HA, Scer\GAL4GMR.PF

      (Park et al., 2010)
      Phenotype Manifest In
      Suppressed by
      NOT Suppressor of
      Statement
      Reference

      porin[+]/porinA2 is a non-suppressor of eye phenotype of DebclUAS.Tag:HA, Scer\GAL4GMR.PF

      (Park et al., 2010)

      porin[+]/porinA2 is a non-suppressor of eye disc phenotype of DebclUAS.Tag:HA, Scer\GAL4GMR.PF

      (Park et al., 2010)

      porinA2/porinA2 is a non-suppressor of eye phenotype of DebclUAS.Tag:HA, Scer\GAL4GMR.PF

      (Park et al., 2010)

      porinA2/porinA2 is a non-suppressor of eye disc phenotype of DebclUAS.Tag:HA, Scer\GAL4GMR.PF

      (Park et al., 2010)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      porinA2/+ or porinA2/porinA2 does not suppress the small eye phenotype seen in animals expressing debclScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PF. The increased apoptosis seen in the eye discs of these animals is also not suppressed by either porinA2/+ or porinA2/porinA2.

      The defect in mitochondrial morphology in the indirect flight muscles which is seen in porinA2 homozygotes is suppressed by expression of either Drp1Scer\UAS.T:Ivir\HA1 or MarfGD11094 under the control of Scer\GAL4Mef2.PR.

      The defects in flight ability that are seen in porinA2 flies 20 days after eclosion are rescued by expression of Drp1Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4Mef2.PR.

      (Park et al., 2010)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      porinA2 is rescued by porinUAS.Tag:FLAG/Scer\GAL4Tub.PU

      (Park et al., 2010)
      Comments

      Expression of porinScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4tub.PU rescues the defects in climbing and flight ability that are seen in porinA2 flies 20 days after eclosion.

      Expression of porinScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4tub.PU rescues the sterility of porinA2 homozygous males.

      Expression of porinScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4tub.PU significantly rescues the defects in mitochondrial morphology in the indirect flight muscles that are seen in porinA2 homozygotes.

      (Park et al., 2010)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      PorinA2
      (Ham et al., 2020, Lee et al., 2018)
      porinA2
      (Lee et al., 2016, Park et al., 2010)
      Name Synonyms
      Secondary FlyBase IDs
        References (4)