The 5' half of roX1Δ3.Hsp83 is ligated to the 3' portion of roX1Δ9.Hsp83 using the NheI site placed at the deletion point, resulting in a 1.2kb fragment.
lncRNA:roX1Δ39.Hsp83 rescues Df(1)52/lncRNA:roX1MB710
The presence of roX1Δ39 rescues roX1MB710 Df(1)52 male viability.