FlyBase Allele Report: Dmel\lncRNA:roX1[MB710]

General Information

Symbol

Dmel\lncRNA:roX1^MB710

Species

D. melanogaster

Name

FlyBase ID

FBal0056222

Feature type

allele

Associated gene

Dmel\lncRNA:roX1

Associated Insertion(s)

P{lArB}lncRNA:roX1^MB710

Carried in Construct

Also Known As

roX1^mb710

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Meller et al., 1997, Meller et al., 1996, Roman et al., 1996)

Progenitor genotype

Associated Insertion(s)

P{lArB}lncRNA:roX1^MB710

Cytology

Description

Insertion in the roX1 gene about 1.4kb from the 5' end.

(Rattner and Meller, 2004)

Insertion of P{lArB} element into roX1.

(Meller et al., 1997)

Allele components

Component

Use(s)

Inserted element

P{lArB}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

X:3,861,204..3,861,204 [+]

(Deng et al., 2005, Rattner and Meller, 2004, Meller and Rattner, 2002, Kelley et al., 1999, Meller et al., 1997, Meller et al., 1996, Roman et al., 1996)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

AB051842

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

fertile

(Meller et al., 1997)

lethal

(Kelley et al., 2008)

viable

(Meller et al., 1997)

Phenotype Manifest In

Detailed Description

Statement

Reference

Normal male development is seen in roX1^MB710 animals.

(Meller and Rattner, 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Df(1)52/lncRNA:roX1^MB710 has lethal phenotype, suppressible by lncRNA:roX1::Dpse\roX1^{Δ39pseudo.Hsp83}

(Kelley et al., 2008)

Other

Statement

Reference

Df(1)52, Polr2A^+tcos4Δ4.3, lncRNA:roX1^MB710 has partially lethal - majority die | male phenotype

(Deng et al., 2005)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Only 5% of roX1^MB710 Df(1)52 hemizygous males with P{w⁺4Δ4.3} survive to adulthood, but these animals are abundant and healthy at late third instar.

(Deng et al., 2005)

Xenogenetic Interactions

Statement

Reference

The presence of roX1::Dpse\roX1^{Δ39pseudo.Hsp83} rescues roX1^MB710 Df(1)52 male viability.

(Kelley et al., 2008)

Complementation and Rescue Data

Rescued by

Df(1)52/lncRNA:roX1^MB710 is rescued by lncRNA:roX1^Δ39.Hsp83

(Kelley et al., 2008)

Partially rescued by

Df(1)52/lncRNA:roX1^MB710 is partially rescued by lncRNA:roX1^-Reg1.Hsp83

(Kelley et al., 2008)

Df(1)52/lncRNA:roX1^MB710 is partially rescued by lncRNA:roX1^-IRB.Hsp83

(Kelley et al., 2008)

Df(1)52/lncRNA:roX1^MB710 is partially rescued by lncRNA:roX1^-SL2.Hsp83

(Kelley et al., 2008)

Df(1)52/lncRNA:roX1^MB710 is partially rescued by lncRNA:roX1^-AB.Hsp83

(Kelley et al., 2008)

Df(1)52/lncRNA:roX1^MB710 is partially rescued by lncRNA:roX1^-RB1.Hsp83

(Kelley et al., 2008)

Df(1)52/lncRNA:roX1^MB710 is partially rescued by lncRNA:roX1^-RB2.Hsp83

(Kelley et al., 2008)

Df(1)52/lncRNA:roX1^MB710 is partially rescued by lncRNA:roX1^-RB3.Hsp83

(Kelley et al., 2008)

Not rescued by

Df(1)52/lncRNA:roX1^MB710 is not rescued by Scer\GAL4^Act5C.PU/lncRNA:roX1^UAS.cKa

(Kelley et al., 2008)

Df(1)52/lncRNA:roX1^MB710 is not rescued by lncRNA:roX1^-SL1.Hsp83

(Kelley et al., 2008)

Df(1)52/lncRNA:roX1^MB710 is not rescued by lncRNA:roX1^-RB123.Hsp83

(Kelley et al., 2008)

Df(1)52/lncRNA:roX1^MB710 is not rescued by lncRNA:roX1^-3RB.Hsp83

(Kelley et al., 2008)

Comments

The presence of roX1^Scer\UAS.cKa (without a Scer\GAL4 driver) fails to rescue the viability found in roX1^MB710 Df(1)52 double mutant males. When the polytene chromosomes of these dying males are examined, the X chromosome exhibits collapsed morphology and very low levels of bound MSL proteins. However, the autosomal region around the transgene insertion site (within 1-2Mbp) is heavily coated with MSL complex in all nuclei.

The presence of roX1^Δ39 rescues roX1^MB710 Df(1)52 male viability.

The presence of roX1^ΔReg1.Hsp83 partially rescues roX1^MB710 Df(1)52 male viability to 47% of wild-type levels.

The presence of roX1^ΔSL1.Hsp83 fails to rescue roX1^MB710 Df(1)52 male viability to wild-type levels.

The presence of roX1^ΔIRB.Hsp83 partially rescues roX1^MB710 Df(1)52 male viability to 62% of wild-type levels.

The presence of roX1^ΔIRB.Hsp83 partially rescues roX1^MB710 Df(1)52 male viability to 10% of wild-type levels.

The presence of roX1^ΔAB.Hsp83 partially rescues roX1^MB710 Df(1)52 male viability to 12% of wild-type levels.

The presence of roX1^ΔRB1.Hsp83 partially rescues roX1^MB710 Df(1)52 male viability to 38% of wild-type levels.

The presence of roX1^ΔRB2.Hsp83 partially rescues roX1^MB710 Df(1)52 male viability to 60% of wild-type levels.

The presence of roX1^ΔRB3.Hsp83 partially rescues roX1^MB710 Df(1)52 male viability to 47% of wild-type levels.

The presence of roX1^{ΔRB123.Hsp83} fails to rescue roX1^MB710 Df(1)52 male viability to wild-type levels.

The presence of roX1^{ΔRB1-3.Hsp83} fails to rescue roX1^MB710 Df(1)52 male viability to wild-type levels.

(Kelley et al., 2008)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

MB710 P{lArB} element resides in a transcribed region that affects yin and roX1 expression.

(Roman et al., 1996)

External Crossreferences and Linkouts ( 1 )

Crossreferences

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

AB051842

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

MB710

(Franke and Baker, 1999)

lncRNA:roX1^MB710

roX1^MB710

(Kelley et al., 2008, Deng et al., 2005)

roX1^mb710

(Rattner and Meller, 2004, Meller and Rattner, 2002)

rox1^MB710

(Franke and Baker, 1999)

roxMB710

(Franke and Baker, 2000)

yang^MB710

(Meller et al., 1996, Roman et al., 1996)

Name Synonyms

Secondary FlyBase IDs

FBal0046868

References (13)

Report Sections

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