FlyBase Allele Report: Dmel\Slimp[RNAi.UAS]

General Information

Symbol

Dmel\Slimp^RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0248987

Feature type

allele

Associated gene

Dmel\Slimp

Associated Insertion(s)

Carried in Construct

P{UAS-Slimp.RNAi}

Key Links

Transgenic product class

RNAi_reagent

(Guitart et al., 2010)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Guitart et al., 2010)

Mutagen

in vitro construct

(Guitart et al., 2010)

Progenitor genotype

Carried in construct

P{UAS-Slimp.RNAi}

Cytology

Description

UAS drives expression of a 566-bp fragment of Slimp cDNA in an inverted repeat.

(Guitart et al., 2010)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Slimp

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell number, with Scer\GAL4^ptc-559.1

(Guitart et al., 2010)

lethal | heat sensitive, with Scer\GAL4^Act5C.PU

(Guitart et al., 2010)

Phenotype Manifest In

anterior crossvein | cell autonomous, with Scer\GAL4^ptc-559.1

(Guitart et al., 2010)

wing cell | cell autonomous, with Scer\GAL4^ptc-559.1

(Guitart et al., 2010)

Detailed Description

Statement

Reference

Scer\GAL4^Act5C.PU-mediated expression of Slimp^{dsRNA.Scer\UAS} significantly reduces viability: only 41% of flies emerge. Lethality is increased at higher temperatures. Lethality is significantly suppressed by addition of an anti-oxidant mix in the diet.

Scer\GAL4^ptc-559.1-mediated expression of Slimp^{dsRNA.Scer\UAS} results in a narrowing of the region flanked by longitudinal veins L3 and L4 in the adult wing. This is caused by a reduction in the number of cells and not a reduction in cell size. The anterior cross vein is reduced/lost in some cases.

The relative amount of mitochondrial DNA in Scer\GAL4^Act5C.PU, Slimp^{dsRNA.Scer\UAS} larvae is not increased compared to controls.

(Guitart et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^Act5C.PU, Slimp^RNAi.UAS has lethal | heat sensitive phenotype, enhanceable by Dcr-2^UAS.cDa, Scer\GAL4^Act5C.PU

(Guitart et al., 2010)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^ptc-559.1, Slimp^RNAi.UAS has wing cell | cell autonomous phenotype, enhanceable by Dcr-2^UAS.cDa/Scer\GAL4^nub-AC-62

(Guitart et al., 2010)

Other

Statement

Reference

Dcr-2^UAS.cDa, Scer\GAL4^nub-AC-62, Slimp^RNAi.UAS has wing phenotype

(Guitart et al., 2010)

Dcr-2^UAS.cDa, Scer\GAL4^Act5C.PU, Slimp^RNAi.UAS has mitochondrial crista phenotype

(Guitart et al., 2010)

Dcr-2^UAS.cDa, Scer\GAL4^Act5C.PU, Slimp^RNAi.UAS has mitochondrial matrix phenotype

(Guitart et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Dcr-2^Scer\UAS.cDa intensifies the lethality caused by Scer\GAL4^Act5C.PU-mediated expression of Slimp^{dsRNA.Scer\UAS} : only 4% (rather than 41%) of flies emerge. Lethality is increased at higher temperatures. Lethality is not suppressed by addition of an anti-oxidant mix in the diet.

Scer\GAL4^nub-AC-62-mediated co-expression of Slimp^{dsRNA.Scer\UAS} and Dcr-2^Scer\UAS.cDa results in failure of the wing to unfold, though the overall structure of the wing is not compromised.

Co-expression of Dcr-2^Scer\UAS.cDa further reduces the narrowing of the region flanked by longitudinal veins L3 and L4 in the adult wing caused by Scer\GAL4^ptc-559.1-mediated expression of Slimp^{dsRNA.Scer\UAS}.

Scer\GAL4^Act5C.PU-mediated co-expression of Slimp^{dsRNA.Scer\UAS} and Dcr-2^Scer\UAS.cDa results in local/complete loss of cristae structures in mitochondrial inner membranes and swollen and less electron dense mitochondrial matrixes in larval fat bodies. The relative amount of mitochondrial DNA in these larvae is increased compared to controls: mitochondrial density and average size is increased.

Scer\GAL4^Act5C.PU-mediated co-expression of Slimp^{dsRNA.Scer\UAS} and Dcr-2^Scer\UAS.cDa results in considerable reduction of mitochondrial respiratory values (normalized by mitochondrial density).

(Guitart et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Slimp^RNAi.UAS

Slimp^{dsRNA.Scer\UAS}

Slimp^dsRNA.UAS

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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