FlyBase Allele Report: Dmel\Pfk[UAS.cTa]

General Information

Symbol

Dmel\Pfk^UAS.cTa

Species

D. melanogaster

Name

FlyBase ID

FBal0256730

Feature type

allele

Associated gene

Dmel\Pfk

Associated Insertion(s)

Carried in Construct

P{UAS-Pfk.T}

Also Known As

UAS-PFK

Key Links

Transgenic product class

wild_type

(Tennessen et al., 2011)

Mutagen

in vitro construct - regulatory fusion

Nature of the Allele

Transgenic product class

wild_type

(Tennessen et al., 2011)

Mutagen

in vitro construct - regulatory fusion

(Tennessen et al., 2011)

Progenitor genotype

Carried in construct

P{UAS-Pfk.T}

Cytology

Description

UASt regulatory sequences drive expression of Pfk.

(Tennessen et al., 2011)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Pfk

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Huntington's disease

modeled by Hsap\HTT^Q93.ex1.UAS

(Besson et al., 2015)

DOES NOT ameliorate Leigh disease

modeled by ND-23^KK102085

(Cabirol-Pol et al., 2018)

ameliorates amyotrophic lateral sclerosis type 10

modeled by Hsap\TARDBP^{G298S.UAS.YFP}

(Manzo et al., 2019)

modeled by Hsap\TARDBP^UAS.YFP.cEa

(Manzo et al., 2019)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Pfk^UAS.cTa, Scer\GAL4^elav-C155 is a suppressor of short lived phenotype of Pdha^GD12103, Scer\GAL4^elav-C155

(Besson et al., 2015)

Pfk^UAS.cTa, Scer\GAL4^elav-C155 is a suppressor of short lived phenotype of ND-23^KK102085, Scer\GAL4^elav-C155

(Besson et al., 2015)

NOT Suppressor of

Statement

Reference

Pfk^UAS.cTa, Scer\GAL4^elav.PU is a non-suppressor of short lived phenotype of ND-23^KK102085, Scer\GAL4^elav.PU

(Cabirol-Pol et al., 2018)

Pfk^UAS.cTa, Scer\GAL4^elav.PU is a non-suppressor of abnormal neuroanatomy | adult stage | progressive phenotype of ND-23^KK102085, Scer\GAL4^elav.PU

(Cabirol-Pol et al., 2018)

Pfk^UAS.cTa, Scer\GAL4^elav.PU is a non-suppressor of decreased cell number | adult stage | progressive phenotype of ND-23^KK102085, Scer\GAL4^elav.PU

(Cabirol-Pol et al., 2018)

Pfk^UAS.cTa, Scer\GAL4^elav-C155 is a non-suppressor of short lived phenotype of Hsap\HTT^Q93.ex1.UAS, Hsap\SLC2A3^UAS.cBa, Scer\GAL4^elav-C155

(Besson et al., 2015)

Pfk^UAS.cTa, Scer\GAL4^elav-C155 is a non-suppressor of abnormal neuroanatomy phenotype of Hsap\HTT^Q93.ex1.UAS, Hsap\SLC2A3^UAS.cBa, Scer\GAL4^elav-C155

(Besson et al., 2015)

Phenotype Manifest In

Suppressor of

Statement

Reference

Pfk^UAS.cTa, Scer\GAL4^elav-C155 is a suppressor of ommatidium phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^elav-C155

(Besson et al., 2015)

NOT Suppressor of

Statement

Reference

Pfk^UAS.cTa, Scer\GAL4^elav.PU is a non-suppressor of ommatidium | progressive phenotype of ND-23^KK102085, Scer\GAL4^elav.PU

(Cabirol-Pol et al., 2018)

Pfk^UAS.cTa, Scer\GAL4^elav.PU is a non-suppressor of photoreceptor neuron | progressive phenotype of ND-23^KK102085, Scer\GAL4^elav.PU

(Cabirol-Pol et al., 2018)

Pfk^UAS.cTa, Scer\GAL4^repo.PU is a non-suppressor of adult brain phenotype of ND-23^KK102085, Scer\GAL4^repo.PU

(Cabirol-Pol et al., 2018)

Pfk^UAS.cTa, Scer\GAL4^repo.PU is a non-suppressor of lipid droplet | adult stage phenotype of ND-23^KK102085, Scer\GAL4^repo.PU

(Cabirol-Pol et al., 2018)

Pfk^UAS.cTa, Scer\GAL4^elav-C155 is a non-suppressor of eye photoreceptor cell phenotype of Hsap\HTT^Q93.ex1.UAS, Hsap\SLC2A3^UAS.cBa, Scer\GAL4^elav-C155

(Besson et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

The decreased lifespan and the ommatidium defects observed in adults expressing ND-23^KK102085 under the control of Scer\GAL4^elav.PU, as well as the increased number and size of lipid droplets in the brain induced by the expression of ND-23^KK102085 under the control of Scer\GAL4^repo.PU, are not suppressed by the co-expression of Pfk^UAS.cTa.

(Cabirol-Pol et al., 2018)

Co-expression of Pfk^Scer\UAS.cTa significantly improves the survival of flies expressing Scer\GAL4^elav-C155>ND-23^KK102085.

Co-expression of Pfk^Scer\UAS.cTa significantly improves the survival of flies expressing Scer\GAL4^elav-C155>l(1)G0334^GD12103.

(Besson et al., 2015)

Expression of Pgi^Scer\UAS.cTa and Pfk^Scer\UAS.cTa under the control of Scer\GAL4^da.G32 rescues the elevated trehalose levels of ERR¹/ERR² mutant larvae. 8% of rescued larvae complete larval development an pupariate, compared to 1% of ERR¹/ERR² control animals.

(Tennessen et al., 2011)

Xenogenetic Interactions

Statement

Reference

Overexpression of Pfk^Scer\UAS.cTa under the control of Scer\GAL4^elav-C155 does not change the survival of flies also expressing Hsap\HTT^{Q93.ex1p.Scer\UAS}. However, statistical analysis of pseudopupil data shows that Pfk^Scer\UAS.cTa overexpression prevents neurodegeneration at day and day 4.

Co-expression of Pfk^Scer\UAS.cTa has no additional effect on the survival or degeneration of photoreceptors in flies expressing Scer\GAL4^elav-C155>Hsap\HTT^{Q93.ex1p.Scer\UAS}.

(Besson et al., 2015)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

60675

w¹¹¹⁸; P{UAS-Pfk.T}3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Pfk^Scer\UAS.cTa

Pfk^UAS.cTa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of

(Tennessen et al., 2011)

Tennessen

(Tennessen et al., 2011)

Secondary FlyBase IDs

References (5)

Report Sections

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