UAS regulatory sequences drive expression of a short inverted repeat.
Adulthood-specific expression of awdHMS00591 under the combined control of Scer\GAL4esg.PU and Scer\GAL80Su(H).GBE (together with Gal80[ts] for the temporal regulation of expression) prevents re-establishment of quiescence (phospho-H3) in the adult gut upon Ecc15 bacterial infection, leading to dysplasia 2 days after infection; upon Pseudomonas entomophila infection, there is an increase in gut barrier dysfunction (in Smurf assays) and increased mortality, as compared to controls.
Adulthood-specific expression of awdHMS00591 under the control of Scer\GAL4esg.PU (together with Gal80[ts] for the temporal regulation of expression) leads to an increased mitotic index (phospho-H3) in the adult gut upon Ecc15 bacterial infection; upon Pseudomonas entomophila infection, there is an increase in gut barrier dysfunction (in Smurf assays).
Scer\GAL4esg.PU, Scer\GAL80Su(H).GBE, awdHMS00591 has increased occurrence of cell division | adult stage | conditional phenotype, non-enhanceable by tkvUAS.D.EGFP/Scer\GAL4esg.PU, Scer\GAL80Su(H).GBE
Scer\GAL4esg.PU, Scer\GAL80Su(H).GBE, awdHMS00591 has increased occurrence of cell division | adult stage | conditional phenotype, suppressible by tkvQ253D.UAS.cNb/Scer\GAL4esg.PU, Scer\GAL80Su(H).GBE
Scer\GAL4esg.PU, Scer\GAL80Su(H).GBE, awdHMS00591 has increased occurrence of cell division | adult stage | conditional phenotype, non-suppressible by tkvUAS.D.EGFP/Scer\GAL4esg.PU, Scer\GAL80Su(H).GBE
Scer\GAL4esg.PU, awdHMS00591 has abnormal immune response | adult stage phenotype, non-suppressible by tkvQ253D.UAS.cNb/Scer\GAL4esg.PU
Scer\GAL4esg.PU, awdHMS00591 has short lived | conditional phenotype, non-suppressible by tkvQ253D.UAS.cNb/Scer\GAL4esg.PU
Scer\GAL4esg.PU, Scer\GAL80Su(H).GBE, awdHMS00591 has adult gut | conditional phenotype, non-enhanceable by tkvUAS.D.EGFP/Scer\GAL4esg.PU, Scer\GAL80Su(H).GBE
Scer\GAL4esg.PU, Scer\GAL80Su(H).GBE, awdHMS00591 has adult gut | conditional phenotype, suppressible by tkvQ253D.UAS.cNb/Scer\GAL4esg.PU, Scer\GAL80Su(H).GBE
Scer\GAL4esg.PU, Scer\GAL80Su(H).GBE, awdHMS00591 has adult gut | conditional phenotype, non-suppressible by tkvUAS.D.EGFP/Scer\GAL4esg.PU, Scer\GAL80Su(H).GBE
Scer\GAL4esg.PU, awdHMS00591 has adult gut | conditional phenotype, non-suppressible by tkvQ253D.UAS.cNb/Scer\GAL4esg.PU
Scer\GAL4esg.PU, awdHMS00591 has gut epithelium | conditional phenotype, non-suppressible by tkvQ253D.UAS.cNb/Scer\GAL4esg.PU