UAS regulatory sequences drive expression of a short inverted repeat.
Expression of SxlHMS00609 under the control of Scer\GAL4VP16.nos.UTR increases the number of single cells and 2-cell cysts and reduces the number of 16-cell cysts in the female germarium compared to controls.
The expression of SxlHMS00609 under the control of Scer\GAL4VP16.otu leads to strong increases in the numbers of germline stem cells and of spectrosomes, as compared to controls.
The expression of SxlHMS00609 leads to severely smaller wings (under the control of Scer\GAL4nub-AC-62 ) and eyes (under the control of Scer\GAL4ey.PH) and leads to a severe increase in apoptosis (TUNEL assay), basal delamination and a decrease in size of the wing disc posterior compartment (under the control of Scer\GAL4e22c), as compared to controls.
Under control conditions, the adulthood-only expression of SxlHMS00609 under the control of Scer\GAL4esg-NP7397 (and Gal80[ts], for the temporal regulation of expression) does not significantly affect the overall mitotic index in the female and male adult posterior midgut, as compared to controls; expression in clones under the control of Scer\GAL4tub.PU leads to a significant decrease in clone cell number at the posterior midgut in females only at 15 days after clone induction, but not at 5 days after clone induction, as compared to control clones.
Scer\GAL4VP16.nanos.UTR, SxlHMS00609 has abnormal cell number | adult stage phenotype, suppressible | partially by NHP2HMC03339, Scer\GAL4VP16.nanos.UTR
BacA\p35UAS.cHa, Scer\GAL4ap-md544, SxlHMS00609 has abnormal size | female | larval stage phenotype, suppressible by msl-1RNAi.UAS.cUa, Scer\GAL4ap-md544
Scer\GAL4e22c, SxlHMS00609 has increased cell death | female limited | larval stage phenotype, suppressible by msl-1RNAi.UAS.cUa/Scer\GAL4e22c
Scer\GAL4e22c, SxlHMS00609 has abnormal size | female limited | larval stage phenotype, suppressible by msl-1RNAi.UAS.cUa/Scer\GAL4e22c
SxlHMS00609, Scer\GAL4VP16.nanos.UTR is an enhancer of abnormal cell number | adult stage phenotype of NHP2HMC03339, Scer\GAL4VP16.nanos.UTR
SxlHMS00609/Scer\GAL4e22c is an enhancer | female of increased cell death | larval stage phenotype of Scer\GAL4e22c, rodGD8768
SxlHMS00609, Scer\GAL4ey.PH is an enhancer | male of abnormal size | adult stage phenotype of Bub3GD9924, Scer\GAL4ey.PH
SxlHMS00609, Scer\GAL4ey.PH is an enhancer | male of visible phenotype of Bub3GD9924, Scer\GAL4ey.PH
BacA\p35UAS.cHa, Scer\GAL4ap-md544, SxlHMS00609 has abnormal size | female | larval stage phenotype
Scer\GAL4VP16.nanos.UTR, SxlHMS00609 has female germline cyst phenotype, suppressible | partially by NHP2HMC03339, Scer\GAL4VP16.nanos.UTR
BacA\p35UAS.cHa, Scer\GAL4ap-md544, SxlHMS00609 has wing disc | female phenotype, suppressible by msl-1RNAi.UAS.cUa, Scer\GAL4ap-md544
Scer\GAL4e22c, SxlHMS00609 has wing disc posterior compartment | female limited phenotype, suppressible by msl-1RNAi.UAS.cUa/Scer\GAL4e22c
SxlHMS00609, Scer\GAL4VP16.nanos.UTR is an enhancer of female germline cyst phenotype of NHP2HMC03339, Scer\GAL4VP16.nanos.UTR
SxlHMS00609/Scer\GAL4e22c is an enhancer | female of wing disc posterior compartment phenotype of Scer\GAL4e22c, rodGD8768
SxlHMS00609, Scer\GAL4ey.PH is an enhancer | male of eye phenotype of Bub3GD9924, Scer\GAL4ey.PH
BacA\p35UAS.cHa, Scer\GAL4ap-md544, SxlHMS00609 has wing disc | female phenotype
BacA\p35UAS.cHa, Scer\GAL4Act5C.PI, SxlHMS00609 has wing disc | female | somatic clone phenotype
Supplemental Dataset_S01.